Molecular Nutrition

Effects of Long-Term Feeding Diets with Different Protein Levels on Gene Expressions Related to Lipid Metabolism of Pigs

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  • State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2017-03-28

  Online published: 2017-09-28

Abstract

This experiment was conducted to study the effects of long-term feeding diets with different protein levels on gene expressions related to lipid metabolism of pigs. Eighteen cross-bred (Duroc×Landrace×Yorkshire) piglets weaned 28 days of age were randomly assigned to 3 groups with 6 replicates per group and 1 piglet per replicate. Piglets in control group[(high crude protein, HCP) group] were fed a diet based on the National Research Council (NRC) (2012) recommendation, those in experimental groups were fed diets with decreasing 3%[(medium crude protein, MCP) group] and 6% crude protein levels[(low crude protein, LCP) group], respectively, based on NRC (2012), and supplemented with 4 essential amino acids, which were lysine, methionine, threonine and tryptophane. The experiment lasted for 125 days. The results showed as follows:1) in liver, compared with HCP group, LCP group significantly decreased expressions of fatty acid synthesis genes including acetyl CoA carboxylase (ACC), fatty acid synthase (FAS), malicenzyme 1 (ME1) and ankyrin 1 (ANK1) (P<0.05), and significantly increased expressions of fatty acid transport genes like peroxisome proliferator-activated receptor gamma (PPARγ) and fatty acid binding proteins (FABP) (P<0.05). However, there were no significant differences between MCP and HCP groups in hepatic expressions of fatty acid synthesis genes and fatty acid transport genes (P>0.05). Compared with HCP group, there were significant increases in hepatic expressions of fatty acid oxygenolysis genes like peroxisome proliferator-activated receptor alfa (PPARα) and carnitine palmitoyltransferase (CPT) in MCP and LCP groups (P<0.05). 2) In the longissimus dorsis muscle (LDM), the expressions of fatty acid synthesis genes like sterol regulatory element binding protein (SREBP), FAS and stearoyl CoA desaturase (SCD) showed significantly higher in MCP group than those in the other two groups (P<0.05), but ACC and FABP displayed significantly lower expressions in LCP group than those in the other two groups (P<0.05). The expression of CPT gene and intramuscular fat (IMF) content of LDM were significantly lower in LCP and MCP groups compared to HCP group (P<0.05). 3) In liver and LDM, there were no significant differences in expressions of adipose triglyceride lipase (ATGL) and 2,4-dienoyl-CoA reductase (DECR) genes among three groups (P>0.05). In conclusion, decreasing dietary crude protein level (3%) properly can promote expressions of fatty acid synthesis and transport genes, but has no significant effects on hepatic expressions of fatty acid synthesis genes; decreasing dietary crude protein level (3%) properly can decrease hepatic expressions of fatty acid oxygenolysis genes, but does not affect IMF content of LDM.

Cite this article

TIAN Zhimei, MA Xianyong, WANG Li, XIONG Yunxia, QIU Yueqin, YANG Xuefen, GAO Kaiguo, WEN Xiaolu, HU Youjun, JIANG Zongyong . Effects of Long-Term Feeding Diets with Different Protein Levels on Gene Expressions Related to Lipid Metabolism of Pigs[J]. Chinese Journal of Animal Nutrition, 2017 , 29(10) : 3761 -3772 . DOI: 10.3969/j.issn.1006-267x.2017.10.039

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