Molecular Nutrition

Effects of Lipopolysaccharide on Rumen Fermentation Investigated by in Vitro Method

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  • 1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. Beijing Key Laboratory for Dairy Cow Nutrition, Beijing University of Agriculture, Beijing 102206, China

Received date: 2017-03-15

  Online published: 2017-09-28

Abstract

This experiment was conducted to examine the effects of lipopolysaccharide (LPS) on rumen fermentation by in vitro method. Four healthy Holstein cows fitted with permanent ruminal cannulas were used as the donor of rumen fluid. Two levels of LPS[0(control group) and 100 000 EU/mL (experimental group)] were supplemented in substrates of different groups. Samples were collected after being cultured for 2, 4, 8, 12 and 24 h, respectively, to determine pH and concentrations of volatile fatty acids, ammonia nitrogen and microbial protein of fermentation fluid. The results showed as follows:with the extension of fermentation time, fermentation fluid pH in control group and experimental group were gradually decreased,the concentrations of total volatile fatty acids, ammonia nitrogen and microbial protein, and contents of acetic acid, propionic acid and butyric acid were gradually increased, and compared with control group, experimental group showed no significant changes (P>0.05). Compared with control group, fermentation fluid pH in experimental group showed a downward trend at 8 and 24 h (0.05 ≤ P < 0.10), and the concentration of ammonia nitrogen showed a downward trend at 4, 8 and 24 h (0.05 ≤ P < 0.10). The results indicate that the supplementation of LPS has a tendency to decrease pH and ammonia nitrogen concentration in fermentation fluid under in vitro culture conditions, but the difference is not significant.

Cite this article

TANG Zhiwen, JIANG Linshu, YANG Liang, WANG Kun, XIONG Benhai . Effects of Lipopolysaccharide on Rumen Fermentation Investigated by in Vitro Method[J]. Chinese Journal of Animal Nutrition, 2017 , 29(10) : 3799 -3806 . DOI: 10.3969/j.issn.1006-267x.2017.10.044

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