Optimization of Carbon and Nitrogen Sources of Clostridium butyricum CBM01 and Its Tolerance to Gastrointerestinal Tract

LIU Lei, TIAN Ya, NI Xueqin, ZENG Dong, PAN Kangcheng, WANG Hesong

doi:10.3969/j.issn.1006-267x.2017.10.047

2017 , Vol. 29 >Issue 10: 3831 - 3836

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.10.047

Molecular Nutrition

Optimization of Carbon and Nitrogen Sources of Clostridium butyricum CBM01 and Its Tolerance to Gastrointerestinal Tract

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Animal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2016-11-01

Online published: 2017-09-28

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Abstract

This experiment was conducted to investigate the optimal carbon and nitrogen source of Clostridium butyricum CBM01 and its tolerance to gastrointestinal tract. The reinforced clostridial medium (RCM) was used to optimize the carbon and nitrogen sources of Clostridium butyricum CBM01 with the method of controlling variables, and the tolerance of Clostridium butyricum CBM01 to gastrointestinal tract was also determined. The results showed as follows:1) the best carbon source was glucose and the best nitrogen source was peptone for Clostridium butyricum CBM01; 2) the survival rates of of Clostridium butyricum CBM01 in the artificial gastric juice and artificial intestinal juice for 3 h were at 90.33% and 92.09%, respectively; 3) the highest tolerable concentration of Clostridium butyricum CBM01 to bile salt was 0.40%. Altogether, as Clostridium butyricum CBM01 exerts great characteristics to tolerate gastrointestinal environments it can be considered as an ideal potential probiotic strain for further researching.

Key words： Clostridium butyricum; carbon sources; nitrogen sources; tolerance of bile salt; artificial gastric juice; artificial intestinal juice

Cite this article

LIU Lei, TIAN Ya, NI Xueqin, ZENG Dong, PAN Kangcheng, WANG Hesong . Optimization of Carbon and Nitrogen Sources of Clostridium butyricum CBM01 and Its Tolerance to Gastrointerestinal Tract[J]. Chinese Journal of Animal Nutrition, 2017 , 29(10) : 3831 -3836 . DOI: 10.3969/j.issn.1006-267x.2017.10.047

References

[1] 王金合.微生态饲料添加剂及其在畜禽生产中的应用研究现状[J].畜牧与饲料科学,2009,30(2):36-38.

[2] SUPRIYATI,HARYATI T,SUSANTI T,et al.Nutritional value of rice bran fermented by Bacillus amyloliquefaciens and humic substances and its utilization as a feed ingredient for broiler chickens[J].Asian-Australasian Journal of Animal Sciences,2015,28(2):231-238.

[3] ZHOU X J,JIN E H,LI S H,et al.Effects of dietary supplementation of probiotics (Bacillus subtilis,Bacillus licheniformis,and Bacillus natto) on broiler muscle development and meat quality[J].Turkish Journal of Veterinary and Animal Sciences,2015,39(2):203-210.

[4] LATESH K,SINGH P K,CHANDRAMONI K M.Effect of dietary supplementation of combination of probiotics on the growth performance and immune response of broiler chickens[J].Animal Nutrition and Feed Technology,2013,13(1):15-25.

[5] KONG Q,HE G Q,JIA J L,et al.Oral administration of Clostridium butyricum for modulating gastrointestinal microflora in mice[J].Current Microbiology,2011,62(2):512-517.

[6] 熊祖明,袁杰利.酪酸梭菌的研究与应用进展[J].中国微生态学杂志,2011,23(12):1143-1145.

[7] 宋增福,吴天星,潘晓东.丁酸梭菌对肠道上皮细胞黏附及对鳗弧菌抑制的研究[J].中国兽药杂志,2006,40(8):9-12.

[8] 李雄彪,马庆英,崔云龙.酪酸梭菌:肠道健康的卫士[M].上海:复旦大学出版社,2008:42-44.

[9] 刘红露,范磊,戴茜茜,等.面包酵母硒与酪酸梭菌制剂对肉鸡生长、抗氧化和肠道菌群的影响[J].浙江农业学报,2015,27(9):1529-1534.

[10] 王腾浩.新型丁酸梭菌筛选及其对断奶仔猪生长性能和肠道功能影响的研究[D].博士学位论文.杭州:浙江大学,2015:15-16.

[11] CHEN X,SUN Y Q,XIU Z L,et al.Stoichiometric analysis of biological hydrogen production by fermentative bacteria[J].International Journal of Hydrogen Energy,2006,31(4):539-549.

[12] 刘亭婷,滑静,王晓霞,等.丁酸梭菌对蛋用仔公鸡肠道菌群、形态结构及黏膜免疫相关细胞的影响[J].动物营养学报,2012,24(11):2210-2221.

[13] WOO T D H,OKA K,TAKAHASHI M,et al.Inhibition of the cytotoxic effect of Clostridium difficile in vitro by Clostridium butyricum MIYAIRI 588 strain[J].Journal of Medical Microbiology,2011,60(11):1617-1625.

[14] ZHANG B,YANG X,GUO Y,et al.Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens[J].Animal,2011,5(12):1909-1915.

[15] SHINNOH M,HORINAKA M,YASUDA T,et al.Clostridium butyricum MIYAIRI 588 shows antitumor effects by enhancing the release of TRAIL from neutrophils through MMP-8[J].International Journal of Oncology,2013,42(3):903-911.

[16] 农业部畜牧业司.中华人民共和国农业部公告第2045号[EB/OL].(2013-12-30)[2016-10-23].http://www.moa.gov.cn/zwllm/tzgg/gg/201401/t20140103_3730193.htm.

[17] 杨金梅,张德纯,刘明方,等.正常人粪便中酪酸梭菌的分离与分子鉴定[J].中国微生态学杂志,2013,25(9):1032-1035,1039.

[18] 施曼玲,戴赟,章玲.丁酸梭菌培养条件的研究[J].杭州师范大学学报:自然科学版,2001,18(4):38-40.

[19] 戚薇,何玉慧,李安东,等.酪酸梭状芽孢杆菌发酵培养基的优化[J].天津科技大学学报,2010,25(2):18-21.

[20] 宋会仪.丁酸梭菌发酵条件的优化及其对美国红鱼免疫指标的影响[D].硕士学位论文.杭州:浙江大学,2007:29-30.

[21] 赵旭冬.丁酸梭菌清液发酵和处理工艺[D].硕士学位论文.武汉:华中农业大学,2011:31-34.

[22] 姜芳芳,李圣杰,丁轲,等.丁酸梭菌C.L24蛋白酶产酶条件的优化[J].家畜生态学报,2016,37(1):63-67.

[23] KUMAR R R,LEE J T,CHO J Y.Fate,occurrence,and toxicity of veterinary antibiotics in environment[J].Journal of the Korean Society for Applied Biological Chemistry,2012,55(6):701-709.

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