Effects of Tryptophan and Rumen-Protected Tryptophan Supplementations on Lactation Performance, Plasma Indexes and Milk Melatonin Content of Dairy Cows

CHEN Junhong, ZHAO Fang, WEI Kaimin, TAN Shixin, YANG Kailun

doi:10.3969/j.issn.1006-267x.2017.11.012

2017 , Vol. 29 >Issue 11: 3921 - 3931

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.012

Molecular Nutrition

Effects of Tryptophan and Rumen-Protected Tryptophan Supplementations on Lactation Performance, Plasma Indexes and Milk Melatonin Content of Dairy Cows

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1. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
2. Xinjiang Tianshan Animal Husbandry Bio-Engineering Co., Ltd., Changji 831100, China

Received date: 2017-05-03

Online published: 2017-10-31

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Abstract

This study was conducted to investigate the effects of tryptophan (Trp) and rumen-protected tryptophan (RPTrp) supplementations in diet on lactation performance, plasma indexes and milk melatonin (MLT) content of dairy cows. Thirty healthy Holstein dairy cows at 3 to 5 years of age, 2 to 3 parities, (180±46) d of lactation days were selected and randomly divided into 3 groups, which were control group, trial groups Ⅰ and Ⅱ. Cows in control group was fed a total mixed ration (TMR), and trial groups Ⅰ and Ⅱ were supplemented with 100 g/(d·head) Trp and 220 g/(d·head) RPTrp[L-tryptophan content was over 45%] based on control group, respectively. The pre-trial lasted for 7 days, and the trial lasted for 60 days. The results showed as follows:1) Compared with control group, milk yield in trial groups Ⅰ and Ⅱ was increased by 11.78% (P<0.05) and 17.19% (P<0.01), respectively; lactose yield in trial groups Ⅰ and Ⅱ was significantly increased (P<0.01). 2) Compared with control group, plasma growth hormone (GH) content at 14:00 and 22:00 in trial groups Ⅰ and Ⅱ was significantly increased(P<0.01); at 14:00, plasma Trp content in trial group Ⅱ was significantly increased (P<0.01), but there was no significantly difference of plasma MLT content among groups (P>0.05); at 22:00, plasma Trp (P<0.01) and MLT contents (P<0.05) in trial group Ⅱ were significantly increased; in trial group Ⅰ, plasma Trp, 5-hydroxytryptamine (5-HT) and MLT contents were not significantly changed both at 14:00 and 22:00 (P>0.05). 3) Compared with control group, at 05:00, milk MLT content in trial group Ⅱ was significantly increased (P<0.01); at 18:00, there was no significant difference of milk MLT content among groups (P>0.05). 4) Compared with control group, plasma total antioxidant capacity (T-AOC) in trial group Ⅰ was significantly increased(P<0.05); plasma glutathione peroxidase (GSH-Px) activity in trial group Ⅱ was significantly increased(P<0.01); plasma superoxide dismutase (SOD) activity in trial groups Ⅰ and Ⅱ was significantly increased (P<0.01); plasma malondialdehyde (MDA) in trial group Ⅱ was significantly reduced (P<0.01). In conclusion, supplementation of Trp[100 g/(d·head)] and RPTrp[220 g/(d·head),] increases milk yield, plasma GH content and antioxidant capacity of Holstein dairy cows, and RPTrp shows better effect; supplement of RPTrp[220 g/(d·head)] elevated plasma Trp content and plasma MLT contents at night of dairy cows, and hence increase MLT content in milk at the night.

Key words： tryptophan; rumen-protected tryptophan; dairy cow; lactation performance; melatonin

Cite this article

CHEN Junhong, ZHAO Fang, WEI Kaimin, TAN Shixin, YANG Kailun . Effects of Tryptophan and Rumen-Protected Tryptophan Supplementations on Lactation Performance, Plasma Indexes and Milk Melatonin Content of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2017 , 29(11) : 3921 -3931 . DOI: 10.3969/j.issn.1006-267x.2017.11.012

References

[1] PÉVET P,BOTHOREL B,SLOTTEN H,et al.The chronobiotic properties of melatonin[J].Cell and Tissue Research,2002,309(1):183-191.

[2] JAN J E,REITER R J,WASDELL M B,et al.The role of the thalamus in sleep,pineal melatonin production,and circadian rhythm sleep disorders[J].Journal of Pineal Research,2009,46(1):1-7.

[3] REITER R J,TAN D X,FUENTES-BROTO L.Melatonin:a multitasking molecule[J].Progress in Brain Research,2010,181(6):127-151.

[4] 张微,赵广永,张晓明,等.天然高褪黑素牛奶生产技术[J].动物营养学报,2016,28(3):635-640.

[5] 白苗苗,刘红南,吴信,等.饲粮中色氨酸对畜禽的免疫调节作用[J].动物营养学报,2016,28(2):361-368.

[6] ESTEBAN S,NICOLAUS C,GARMUNDI A,et al.Effect of orally administered L-tryptophan on serotonin,melatonin,and the innate immune response in the rat[J].Molecular and Cellular Biochemistry,2004,267(1):39-46.

[7] ERIKSSON L,VALTONEN M,LAITINEN J T,et al.Diurnal rhythm of melatonin in bovine milk:pharmacokinetics of exogenous melatonin in lactating cows and goats[J].Acta Veterinaria Scandinavica,1998,39(3):301-310.

[8] ONODERA R.Methionine and lysine metabolism in the rumen and the possible effects of their metabolites on the nutrition and physiology of ruminants[J].Amino Acids,1993,5(2):217-232.

[9] 孔凡德,刘建新,吴跃明.瘤胃保护性氨基酸及其对奶牛生产性能的影响[J].中国奶牛,2001(1):30-32.

[10] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007:48-93.

[11] 傅启高,雒秋江.用邻-甲酚酞比色法测定饲料中钙含量的研究[J].动物营养学报,1996,8(3):25-30.

[12] 谢占武,邓上奇,刘瑞凝,等.健康牛、羊血清及牛奶中游离色氨酸的微量测定[J].中国预防兽医学报,1991(6):27-30.

[13] 李华伟,祝倩,吴灵英,等.色氨酸的生理功能及其在畜禽饲粮中的应用[J].动物营养学报,2016,28(3):659-664.

[14] 刘君,耿忠诚,马慧,等.瘤胃保护性色氨酸对肉羊氮代谢及生长性能的影响[J].饲料与畜牧:新饲料,2010,46(1):18-21.

[15] HUI M,WEI Z,ZHU X P,et al.Effects of rumen-protected tryptophan on growth performance,fibre characteristics,nutrient utilization and plasma essential amino acids in Cashmere goats during the cashmere slow growth period[J].Livestock Science,2010,131(2/3):227-233.

[16] KOLLMANN M T,LOCHER M,HIRCHE F,et al.Effects of tryptophan supplementation on plasma tryptophan and related hormone levels in heifers and dairy cows[J].Domestic Animal Endocrinology,2008,34(1):14-24.

[17] KASUYA E,YAYOU K,HASHIZUME T,et al.A possible role of central serotonin in L-tryptophan-induced GH secretion in cattle[J].Animal Science Journal,2010,81(3):345-351.

[18] 吴妙宗,蔡辉益.动物色氨酸与烟酸互作关系研究进展[J].动物营养学报,2002,14(2):5-8.

[19] LEONARDI C,STEVENSON M,ARMENTANO L E.Effect of two levels of crude protein and methionine supplementation on performance of dairy cows[J].Journal of Dairy Science,2003,86(12):4033-4042.

[20] BACH A,STERN M D.Measuring resistance to ruminal degradation and bioavailability of ruminally protected methionine[J].Animal Feed Science and Technology,2000,84(1/2):23-32.

[21] RHOADS M L,MEYER J P,KOLATH S J,et al.Growth hormone receptor,insulin-like growth factor (IGF)-1,and IGF-binding protein-2 expression in the reproductive tissues of early postpartum dairy cows[J].Journal of Dairy Science,2008,91(5):1802-1813.

[22] FORSYTH I A,WALLIS M.Growth Hormone and prolactin molecular and functional evolution[J].Journal of Mammary Gland Biology and Neoplasia,2002,7(3):291-312.

[23] CHARNEY D S,HENINGER G R,REINHARD R F,Jr,et al.The effect of intravenous L-tryptophan on prolactin and growth hormone and mood in healthy subjects[J].Psychopharmacology,1982,77(3):217-222.

[24] KASUYA E,KUSHIBIKI S,SUTOH M,et al.Effect of melatonin injected into the third ventricle on growth hormone secretion in Holstein steers[J].Journal of Veterinary Medical Science,2006,68(10):1075-1080.

[25] GIBB D J,KLOPFENSTEIN T J,BRITTON R A,et al.Plasma amino acid response to graded levels of escape protein[J].Journal of Animal Science,1992,70(9):2885-2892.

[26] MA H,CHENG J,ZHU X,et al.Effects of rumen-protected tryptophan on performance,nutrient utilization and plasma tryptophan in cashmeregoats[J].African Journal of Biotechnology,2011,10(30):5806-5811.

[27] CHAN J S,SNOEREN E M,CUPPEN E,et al.The serotonin transporter plays an important role in male sexual behavior:a study in serotonin transporter knockout rats[J].Journal of Sexual Medicine,2011,8(1):97-108.

[28] SARWAR G,BOTTING H G.Liquid concentrates are lower in bioavailable tryptophan than powdered infant formulas,and tryptophan supplementation of formulas increases brain tryptophan and serotonin in rats[J].Journal of Nutrition,1999,129(9):1692-1697.

[29] MONTI J M,JANTOS H.The roles of dopamine and serotonin,and of their receptors,in regulating sleep and waking[J].Progress in Brain Research,2008,172:625-646.

[30] SHEN Y B,COFFEY M T,KIM S W.Effects of short term supplementation of L-tryptophan and reducing large neutral amino acid along with L-tryptophan supplementation on growth and stress response in pigs[J].Animal Feed Science and Technology,2015,207:245-252.

[31] 任皓威,王丹,刘鹏,等.光照调整对于牛乳中褪黑素质量浓度的影响及其检测[J].中国乳品工业,2011,39(4):55-57.

[32] CASTRO N,SPENGLER M,VANESSA L,et al.Diurnal pattern of melatonin in blood and milk of dairy cows[J].Milchwissenschaft-Milk Science International,2011,6(4):352-353.

[33] 魏宗友,王洪荣,潘晓花,等.饲喂方式和饲粮色氨酸水平对扬州鹅免疫功能及抗氧化指标的影响[J].动物营养学报,2012,24(12):2356-2365.

[34] 马玉娥,占秀安,朱巧明,等.饲粮色氨酸水平对黄羽肉种鸡生产性能、抗氧化功能及血清生化指标的影响[J].动物营养学报,2011,23(12):2177-2182.

[35] TERRÓN M P,DELGADO J,PAREDES S D,et al.Effect of melatonin and tryptophan on humoral immunity in young and old ringdoves (Streptopelia risoria)[J].Experimental Gerontology,2009,44(10):653-658.

[36] ÖZTURK G,CO? KUN S,ERBA? D,et al.The effect of melatonin on liver superoxide dismutase activity,serum nitrate and thyroid hormone levels[J].The Japanese Journal of Physiology,2000,50(1):149-153.

[37] PASHKOV A N,POPOV S S,SEMENIKHINA A V,et al.Glutathione system and activity of NADPH-generating enzymes in the liver of intact rats and animals with toxic hepatitis receiving melatonin[J].Bulletin of Experimental Biology and Medicine,2005,139(5):565-568.

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