Effects of Dietary Nitrogen Level on Urine Purine Derivatives Excretion and Microbial Nitrogen Production in Yaks

WANG Weiwei, WANG Chuanyang, HAO Lizhuang, LIU Hao, ZHONG Chongliang, ZHOU Jianwei, LONG Ruijun

doi:10.3969/j.issn.1006-267x.2017.11.013

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 11: 3932 - 3941

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.013

Molecular Nutrition

Effects of Dietary Nitrogen Level on Urine Purine Derivatives Excretion and Microbial Nitrogen Production in Yaks

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1. College of Pastoral Agriculture Science and Technology of Lanzhou University, Lanzhou 730000, China;
2. Animal Husbandry and Veterinary Station of Dulan County, Dulan 816199, China;
3. College of Animal Science and Veterinary Medicine of Qinghai University, Xining 810016, China;
4. School of Life Sciences of Lanzhou University, Lanzhou 730000, China

Received date: 2017-04-14

Online published: 2017-10-31

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Abstract

This experiment was conducted to explore the regularity of urine purine derivatives (PD) excretion respond to dietary nitrogen level, and based on this, ruminal microbial nitrogen (MN) production was estimated, thus provided references for appropriate feeding of yaks in cold farming area. Four 3-year-old castrated male yaks[body weight was (192±12) kg] were divided into 4 groups with 1 yak per group in a 4×4 Latin square design. Dietary nitrogen levels of different groups were 1.03%, 1.95%, 2.85% and 3.76%, respectively. The experiment consisted of 4 periods, each lasted for 21 d, allowing a 15-d pre-test period and a 6-d test period. The results showed that urine PD were mainly consist of allantoin and uric acid, allantoin/PD and uric acid/PD were 0.69 to 0.76 and 0.23 to 0.30, respectively, and negligible contents of xanthine and hypoxanthine were found. As dietary nitrogen level increasing, urine PD, allantoin, uric acid and hippuric acid extractions were increased linearly (P<0.05), however, uric acid/PD and purine nitrogen index (PNI) were linearly decreased (P<0.05). With the increase of dietary nitrogen level, ruminal bacterial purine bases (RNA equivalent) content, bacterial nitrogen (BN) content and MN production were linearly increased (P<0.05), and efficiency of dietary nitrogen converted into MN[ruminal MN/nitrogen intake (NI)] was linearly decreased (P<0.05). There were a strong relationship between urine PD extraction (mmol/d), ruminal MN (g/d) and NI (g/d), the following mathematical equations were established as follows:PD=0.58NI+18.28, MN=0.18NI+22.18. In summary, ruminal MN production is the highest (42.60 g/d) when dietary nitrogen level is 2.85%, whereas PNI and efficiency of NI converted into MN are the highest under the condition of low dietary nitrogen level (1.03%). The results indicate the great efficiency of nitrogen utilization when low nitrogen provision in yaks, and their adaptation nutrition mechanism to the deficiencies in intake of nutrient of yaks in Tibetan plateau.

Key words： yak; dietary nitrogen levels; urine purine derivatives; ruminal microbial nitrogen production

Cite this article

WANG Weiwei, WANG Chuanyang, HAO Lizhuang, LIU Hao, ZHONG Chongliang, ZHOU Jianwei, LONG Ruijun . Effects of Dietary Nitrogen Level on Urine Purine Derivatives Excretion and Microbial Nitrogen Production in Yaks[J]. Chinese Journal of Animal Nutrition, 2017 , 29(11) : 3932 -3941 . DOI: 10.3969/j.issn.1006-267x.2017.11.013

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