Effects of Amylose to Amylopectin Ratio in Concentrate on Rumen Bacterial Community in Lambs

YU Yangyang, ZHAO Fangfang, ZHANG Aizhong, JIANG Ning, LI Yanbing, CHEN Yong

doi:10.3969/j.issn.1006-267x.2017.11.015

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 11: 3951 - 3961

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.015

Molecular Nutrition

Effects of Amylose to Amylopectin Ratio in Concentrate on Rumen Bacterial Community in Lambs

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1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2017-05-04

Online published: 2017-10-31

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Abstract

The objective of this study was to determine the effects of amylose to amylopectin ratio in concentrate on rumen bacterial community in lambs at different days of age. Forty-eight male lambs with no significant difference in body weight (P>0.05) were randomly divided into 4 groups, there were 3 replicates per group with 4 lambs per replicate. The concentrate amylose to amylopectin ratio of different groups was 0.12 (tapioca starch), 0.23 (corn starch), 0.24 (wheat starch) and 0.48 (pea starch), respectively. The whole experiment period was 77 days. One lamb from each replicate were randomly selected and slaughtered at 21, 35, 56 and 77 days of age to collect rumen fluid sample. The diversity of rumen bacterial community in lambs was determined by PCR-denaturing gradient gel electrophoresis (DGGE). The results showed that there was no effect on diversity index, evenness and richness of lambs at 21, 35, 56 and 77 days of age(P>0.05). Diversity index in lambs at 21 days of age was significant higher than that at 35 days of age (P<0.05),and it was increased at 56 and 77 days of age, but the numerical values were still lower than that at 21 days of age. Bands recovered from DGGE were almost belonged to Bacteroidetes and Firmicutes. Selenomonas bovis content in rumen in lambs at 56 days of age in wheat starch group was higher. Therefore, different amylose to amylopectin ratio in concentrate do not affect the structure of lamb rumen dominant bacteria, but feed wheat starch diet can promote the growth of Selenomonas bovis in rumen in lambs at 56 days of age.

Key words： amylose to amylopectin ratio; lamb; PCR-denaturing gradient gel electrophoresis; rumen bacterial community; diversity index

Cite this article

YU Yangyang, ZHAO Fangfang, ZHANG Aizhong, JIANG Ning, LI Yanbing, CHEN Yong . Effects of Amylose to Amylopectin Ratio in Concentrate on Rumen Bacterial Community in Lambs[J]. Chinese Journal of Animal Nutrition, 2017 , 29(11) : 3951 -3961 . DOI: 10.3969/j.issn.1006-267x.2017.11.015

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