This study was conducted to investigate effects of dietary cysteamine (CS) on growth performance, nutrient digestibility and nitrogen metabolism of growing minks, and to find the dietary cysteamine appropriate supplemental level and supplemental way. The experimental method of double-factor design was adopted. Fifty-six (86±5)-day-old healthy black short-haired female minks with similar body weight were randomly assigned into 7 groups with 8 replicates per group and 1 mink per replicate. Minks in group Ⅰ (control group) were fed a basal diet; minks in groups Ⅱ, Ⅲ and Ⅳ were fed the basal diet supplemented with 60, 90 and 120 mg/kg CS, respectively, the supplemental way was continuous; minks in groups Ⅴ, Ⅵ and Ⅶ were fed the basal diet supplemented with 60, 90 and 120 mg/kg CS, respectively, the supplemental way was interval (continuous supplemented 1 week, 1 week interval). The preliminary experimental period lasted for 7 days and the experimental period lasted for 53 days. The results showed as follows:1) dietary CS had significantly effects on the average daily feed intake and feed to gain ratio of minks (P<0.01), but had no significant effects on average daily gain (P>0.05). The feed to gain ratio in group Ⅴ was significantly lower than that in groups Ⅰ, Ⅱ and Ⅳ (P<0.01); the average daily gain got the highest value in group Ⅴ, and interval group was higher than continuous group. 2) Dietary CS had significantly effects on the crude protein digestibility and ether extract digestibility of minks (P<0.05), as well as the dry matter digestibility (P<0.01), and the highest values were observed in group Ⅴ. The CS supplemental way had significantly effects on the dry matter digestibility (P<0.01), and the interval group was significantly higher than continuous group (P<0.01). 3) Dietary CS had significantly effects on the nitrogen metabolic indices of minks (P<0.05 or P<0.01). The nitrogen deposition in groups Ⅴ, Ⅵ and Ⅶ was significantly higher than that in group Ⅰ (P<0.05), the net protein utilization and biological value of protein in groups Ⅴ, Ⅵ and Ⅶ were significantly higher than those in group Ⅰ (P<0.01), and the highest values were observed in group Ⅴ. The CS supplemental way had significantly effects on the nitrogen intake content, urinary nitrogen output, net protein utilization and biological value of protein of minks (P<0.01). The nitrogen intake content and urinary nitrogen output of minks in interval group were significantly lower than those in continuous group (P<0.01), the nitrogen deposition in interval group was significantly lower than that in continuous group (P<0.05), the net protein utilization and biological value of protein in interval group were significantly lower than those in continuous group (P<0.01). The CS supplemental level had significantly effects on fecal nitrogen output of minks (P<0.05), and the fecal nitrogen output of minks in 90 mg/kg group was significantly lower than that in 60 mg/kg group (P<0.05). In conclusion, the dietary CS appropriate supplemental level of minks is 60 mg/kg, and the appropriate supplemental way is interval supplementation.