Effects of Montmorillonite on Performance and Cecal Microflora of Laying Hens

CHEN Jifa, PENG Canyang, QU Xiangyong, JI Fang

doi:10.3969/j.issn.1006-267x.2017.11.024

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 11: 4026 - 4035

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.024

Molecular Nutrition

Effects of Montmorillonite on Performance and Cecal Microflora of Laying Hens

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1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China;
3. Amlan International, Chicago IL 60611, USA

Received date: 2017-04-26

Online published: 2017-10-31

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Abstract

This study was conducted to investigate the effects of montmorillonite on performance and cecal microflora of laying hens. Four hundred and eighty Brown Lohmann hens (75 weeks old) were randomly assigned to 5 groups, each of which comprised 8 replicates of 12 hens per replicate. Hens in the control group were fed a basal diet, and hens in experimental groups were fed the basal diets supplemented with 0.3, 0.6, 0.9 and 1.2 g/kg montmorillonite, respectively. The adjustment period lasted for 7 days, and the experimental period lasted for 70 days. The results showed as follows:1) compared with the control group, the daily egg production of hens in 0.9 g/kg montmorillonite group was significantly increased (P<0.05), and feed to egg ratio in experimental groups tended to be higher (P=0.073) than that in the control group during 1 to 5 weeks of the experiment. 2) The quantities of effective sequences and good sequences observed in 0.3, 0.9 and 1.2 g/kg montmorillonite groups were more than those in the control group (P>0.05). 3) The Beta diversity was improved in the experimental groups. The Shannon index in experimental groups tended to be higher than that in the control group (P=0.096), and the Simpson index tended to be lower than that in the control group (P=0.095). 4) At the phylum level, compared with the control group, the relative contents of Firmicutes, Proteobacteria and Spirochaetes in the experimental groups were numerically increased (P>0.05); the relative content of WPS_2 in 0.6 g/kg montmorillonite group was significantly increased (P<0.05), and the Elusimicrobia relative content in 0.3 g/kg montmorillonite group was significantly decreased compared with the control group (P<0.05). At the genus level, compared with the control group, the Ruminococcus relative content in the experimental groups was significantly increased (P<0.05), and the Parabacteroides relative content in experimental groups tended to be higher than that in the control group (P=0.067); the relative contents of Dorea and Blautia in 0.6 and 1.2 g/kg montmorillonite groups were significantly increased (P<0.05); the Paraprevotella relative content in 0.6 g/kg montmorillonite group was significantly increased (P<0.05). 5) The cluster analysis at the genus level showed low similarity of intestinal microflora between the experimental groups and the control group. In conclusion, the bacterial diversity and species abundance of cecal microbiota of laying hens can be partially influenced by montmorillonite, and dietary supplementation with 0.9 g/kg montmorillonite tends to improve the egg production of laying hens.

Key words： montmorillonite; performance; intestinal microbiota; high-throughput sequencing; laying hens

Cite this article

CHEN Jifa, PENG Canyang, QU Xiangyong, JI Fang . Effects of Montmorillonite on Performance and Cecal Microflora of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2017 , 29(11) : 4026 -4035 . DOI: 10.3969/j.issn.1006-267x.2017.11.024

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