Effects of Dietary Chitosan Oligosaccharide and/or Mycotoxin Adsorbent on Growth Performance, Nonspecific Immunity and Disease Resistance of Litopenaeus vannamei

HUANG Qincheng, SHEN Guangrong, TAN Beiping, ZHANG Shuang, HEI Wenjing, DONG Xiaohui

doi:10.3969/j.issn.1006-267x.2017.11.025

2017 , Vol. 29 >Issue 11: 4036 - 4047

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.025

Molecular Nutrition

Effects of Dietary Chitosan Oligosaccharide and/or Mycotoxin Adsorbent on Growth Performance, Nonspecific Immunity and Disease Resistance of Litopenaeus vannamei

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1. Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;
2. Shenzhen Yunong Science & Technology Co., Ltd., Shenzhen 518110, China

Received date: 2017-05-09

Online published: 2017-10-31

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Abstract

An 8-week feed trial was carried out to investigate the effects of single or combined supplementation of chitosan oligosaccharide (COS) and mycotoxin adsorbents (MA) on the growth performance, nonspecific immunity and disease resistance of Litopenaeus vannamei. Six isonitrogenous and isolipid diets were prepared. Firstly, a basal diet was formulated as control diet (C0 group), and then 5 diets were formulated by adding 100 mg/kg COS (C0.1 group), 250 mg/kg COS (C0.25 group), 2 500 mg/kg MA (M2.5 group), 100 mg/kg COS+2 500 mg/kg MA (C0.1+M2.5 group), 250 mg/kg COS+2 500 mg/kg MA (C0.25+M2.5 group), respectively. The Litopenaeus vannamei with an initial average weight of (0.23±0.02) g were randomly assigned into 6 groups with 3 replicates per group and 40 shrimps per replicate. The results showed as follows:1) the weight gain rate (WGR) and specific growth rate (SGR) in C0.1+M2.5 group were significantly higher than those in M2.5 groups (P<0.05), the feed conversion ratio (FCR) in C0.1+M2.5 group was significantly lower than that of other groups (P<0.05) and showed the minimum value, the maximum protein efficiency rate (PER) was occurred in C0.1+M2.5 group and was significantly higher than that of other groups (P<0.05) except C0.25+M0.25 group. The crude protein content of shrimp body in C0.1+M2.5 group was significantly higher than that in C0.1 group (P<0.05), and the crude lipid content of shrimp body in C0.1+M2.5 group was significantly lower than that in C0.1 group (P<0.05). 2) In the serum, the activity of alkaline phosphatase (AKP) in C0.1+M2.5 and C0.25+M2.5 groups was significantly lower than that in C0.1 and C0.25 groups (P<0.05), the activities of superoxide dismutase (SOD) and phenoloxidase (PO) in C0.1+M2.5 group were significantly higher than those in M2.5 and C0.1 groups (P<0.05), and the content of malondialdehyde (MDA) in C0.1+M2.5 group was significantly lower than that in C0.1 and C0.25 groups (P<0.05). In the hepatopancreas, the content of MDA in C0.1+M2.5 group was significantly lower than that in C0.1 group (P<0.05), and the activities of AKP, PO, SOD and lysozyme (LZM) in C0.25+M2.5 group were lower than those in C0.1+M2.5 group, but the contentVibrio harveyid by Vibrio harveyi for the next 7 days, the cumulative mortality rate in C0.1+M2.5 and C0.25+M2.5 groups was significantly lower than that in C0 and M2.5 groups (P<0.05). It can be concluded that adding a certain amount of COS and/or MA in diets can do good to the growth, nonspecific immunity and disease resistance of Litopenaeus vannamei, the combined supplementations of COS and MA show better growth, nonspecific immunity and disease resistance than single supplementation of COS and MA, and the combined supplementation of 100 mg/kg COS and 2 500 mg/kg MA under this experimental condition shows the better effect.

Key words： chitosan oligosaccharide; mycotoxin adsorbent; Litopenaeus vannamei; growth performance; nonspecific immunity; disease resistance

Cite this article

HUANG Qincheng, SHEN Guangrong, TAN Beiping, ZHANG Shuang, HEI Wenjing, DONG Xiaohui . Effects of Dietary Chitosan Oligosaccharide and/or Mycotoxin Adsorbent on Growth Performance, Nonspecific Immunity and Disease Resistance of Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 2017 , 29(11) : 4036 -4047 . DOI: 10.3969/j.issn.1006-267x.2017.11.025

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