Molecular Nutrition

Effects of Replacement of Fish Meal by Soybean Meal on Composition and Protease-Producing Activity of Protease-Producing Aerobic Bacteria in Intestinal Tract of Channa argus

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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2017-04-10

  Online published: 2017-10-31

Abstract

In order to investigate the effects of replacement of fish meal by soybean meal on composition and protease-producing activity of protease-producing aerobic bacteria in intestinal tract of Channa argus, the protease producing bacteria in intestinal tract of Channa argus were isolated and cultured. The selected strains were further quantitatively assayed for the protease-producing activity. One control group (D1 group) diet was formulated with defatted fish meal as the main protein source, and the defatted fish meal addition in this diet was 55%. Next then, two experimental group diets were formulated using soybean meal to replace different ratio of fish meal in control group diet, and the soybean meal in those two diets was 35% (D2 group) and 75% (D3 group), respectively. Each diet was assigned to three replicates of 28 Channa argus with the average body weight of (10.50±0.84) g for 8 weeks in a re-circulated water system indoor. The results showed as follows:The final weight, weight gain rate and specific growth rate was significantly decreased with the replacement ratio of fish meal by soybean meal increasing (P<0.05). A total of 21 protease-producing aerobic bacteria were isolated from the intestine of Channa argus, which was 16 from D1 group, 19 from D2 group and 20 from D3 group. The replacement of fish meal by soybean meal significantly decreased the hydrolysis spot diameter (R)/strain diameter (r) value of strains P1004 and P1018 (P<0.05), which was used to express the protease-producing activity, but significantly increased the R/r value of strains P1009 and P1012 (P<0.05). With the replacement ratio of fish meal by soybean meal increasing, the R/r value of strain P1018 was significantly decreased (P<0.05). Physiological and biochemical characteristics identification and 16S rDNA sequence analysis revealed that the strain P1009, whose protease-producing activity was the highest in the present study, was Pseudomonas aeruginosa. In summary, the results indicate that there exist a great variety of protease-producing aerobic bacteria in intestinal tract of Channa argus, and the replacement of fish meal by soybean meal can affect both the quantity and protease-producing activity of protease-producing bacteria. Strain P1009 which from the intestinal tract of Channa argus has the highest protease-producing activity, and it can be as a potential source of probiotics.

Cite this article

MIAO Shuyan, ZHU Jinyu, ZHAO Chenze, DONG Xiaojing, SUN Longsheng . Effects of Replacement of Fish Meal by Soybean Meal on Composition and Protease-Producing Activity of Protease-Producing Aerobic Bacteria in Intestinal Tract of Channa argus[J]. Chinese Journal of Animal Nutrition, 2017 , 29(11) : 4076 -4084 . DOI: 10.3969/j.issn.1006-267x.2017.11.029

References

[1] GÓMEZ G D,BALCÁZAR J L.A review on the interactions between gut microbiota and innate immunity of fish[J].FEMS Immunology and Medical Microbiology,2008,52(2):145-154.  

[2] LI M,WANG B H,ZHANG M H,et al.Symbiotic gut microbes modulate human metabolic phenotypes[J].Proceedings of the National Academy of Sciences of the United States of America,2008,105(6):2117-2122.  

[3] POKUSAEVA K,FITZGERALD G F,VAN SINDEREN D.Carbohydrate metabolism in Bifidobacteria[J].Genes and Nutrition,2011,6(3):285-306.  

[4] GILL S R,POP M,DEBOY R T,et al.Metagenomic analysis of the human distal gut microbiome[J].Sceience,2006,312(5778):1355-1359.  

[5] BAIRAGI A,GHOSH K S,SEN S K,et al.Enzyme producing bacterial flora isolated from fish digestive tracts[J].Aquaculture International,2002,10(2):109-121.  

[6] 何敏,汪开毓,张宇,等.复合微生物制剂对重口裂腹鱼生长、消化酶活性、肠道菌群及水质指标的影响[J].动物营养学报,2008,20(5):534-539.

[7] 赖凯昭,吕逸欢,梁明振,等.饵料中添加益生菌对奥尼罗非鱼生长性能和肠道蛋白酶活性的影响[J].南方农业学报,2012,43(11):1769-1774.

[8] FENG J B,HU C Q,LUO P,et al.Microbiota of yellow grouper (Epinephelus awoora Temminck & Schlegel, 1842) fed two different diets[J].Aquaculture Research,2010,41(12):1778-1790.  

[9] CEREZUELA R,FUMANAL M,TAPIA-PANIAGUA S T,et al.Histological alterations and microbial ecology of the intestine in gilthead seabream (Sparus aurata L.) fed dietary probiotics and microalgae[J].Cell and Tissue Research,2012,350(3):477-489.  

[10] TORRECILLAS S,MAKOL A,CABALLERO M J,et al.Effects on mortality and stress response in European sea bass,Dicentrarchus labrax (L.),fed mannan oligosaccharides (MOS) after Vibrio anguillarum exposure[J].Journal of Fish Diseases,2012,35(8):591-602.  

[11] YAN Q Y,VAN DER GAST C,YU Y H.Bacterial community assembly and turnover within the intestines of developing Zebrafish[J].PLoS One,2012,7(1):e30603.

[12] 钟蕾,向建国,曾丹,等.饵料对鳡肠道微生物多样性的影响[J].水生生物学报,2016,40(4):830-835.

[13] 周歧存,麦康森,刘永坚,等.动植物蛋白源替代鱼粉研究进展[J].水产学报,2005,29(3):404-410.

[14] FRANCIS G,MAKKAR H P S,BECKER K.Antinutritional factors present in plant-derived alternate fish feed ingredients and their effects in fish[J].Aquaculture,2001,199(3/4):197-227.

[15] QIN J J,LI R Q,RAES J,et al.A human gut microbial gene catalogue established by metagenomic sequencing[J].Nature,2010,464(7285):59-65.  

[16] O'HARA A M,SHANAHAN F.The gut flora as a forgotten organ[J].EMBO Reports,2006,7(7):688-693.  

[17] HARRIS K,KASSIS A,MAJOR G,et al.Is the gut microbiota a new factor contributing to obesity and its metabolic disorders?[J].Journal of Obesity,2012,2012:879151.

[18] 王建建,高权新,张晨捷,等.野生与养殖银鲳消化道菌群结构中产酶菌的对比分析[J].水产学报,2014,38(11):1899-1909.

[19] 杨亚东,杨锡洪,解万翠,等.海水养殖凡纳滨对虾肠道产蛋白酶菌株的筛选、鉴定[J].现代食品科技,2015,31(1):131-136.

[20] LÉSEL R.Does a digestive active bacterial flora exist in fish?[C]//KAUSHIK S J,LUQUET P.Fish nutrition in practice.Biarritz:Ⅳth International Symposium on Fish Nutrition and Feeding,1993,61:655-664.

[21] RAY A K,ROY T,MONDAL S,et al.Identification of gut-associated amylase,cellulase and protease-producing bacteria in three species of Indian major carps[J].Aquaculture Research,2010,41(10):1462-1469.

[22] PARMA L,CANDELA M,SOVERINI M,et al.Next-generation sequencing characterization of the gut bacterial community of gilthead sea bream (Sparus aurata L.) fed low fishmeal based diets with increasing soybean meal levels[J].Animal Feed Science and Technology,2016,222:204-216.

[23] GAJARDO K,JARAMILLO-TORRES A,KORTNER T M,et al.Alternative protein sources in the diet modulate microbiota and functionality in the distal intestine of Atlantic salmon (Salmo salar)[J].Applied and Environmental Microbiology,2017,83(5):e02615-16.

[24] DESAI A R,LINKS M G,COLLINS S A,et al.Effects of plant-based diets on the distal gut microbiome of rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,2012,350/351/352/353:134-142.

[25] REVECO F E,ØVERLAND M,ROMARHEIM O H,et al.Intestinal bacterial community structure differs between healthy and inflamed intestines in Atlantic salmon (Salmo salar L.)[J].Aquaculture,2014,420-421:262-269.

[26] CHYTHANYA R,KARUNASAGAR I,KARUNASAGAR I.Inhibition of shrimp pathogenic vibrios by a marine Pseudomonas Ⅰ-2 strain[J].Aquaculture,2002,208(1/2):1-10.

[27] VIJAYAN K K,SINGH I S B,JAYAPRAKASH N S,et al.A brackishwater isolate of Pseudomonas PS-102,a potential antagonistic bacterium against pathogenic vibrios in penaeid and non-penaeid rearing systems[J].Aquaculture,2006,251(2/3/4):192-200.

[28] 马艳,李婷婷,崔方超,等.大菱鲆源蜂房哈夫尼亚菌群体感应现象及生物被膜调控的研究[J].现代食品科技,2016,32(8):70-76.

[29] 杨求华,郭松林,关瑞章,等.鳗鲡病原性维氏气单胞菌的分离与鉴定[J].生物技术通报,2012(7):134-139.

[30] ORPHAN V J,TAYLOR L T,HAFENBRADL D,et al.Culture-dependent and culture independent characterization of microbial assemblages associated with high-temperature petroleum reservoirs[J].Applied and Environmental Microbiology,2000,66(2):700-711.  

[31] BURKE J D,WEINTRAUB M N,HEWINS C R,et al.Relationship between soil enzyme activities,nutrient cycling and soil fungal communities in a northern hardwood forest[J].Soil Biology and Biochemistry,2011,43(4):795-803.  

[32] RAMIREZ R F,DIXON B A.Enzyme production by obligate intestinal anaerobic bacteria isolated from oscars (Astronotus ocellatus),angelfish (Pterophyllum scalare) and southern flounder (Paralichthys lethostigma)[J].Aquaculture,2003,227(1/2/3/4):417-426.
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