Requirement of Dietary Vitamin E for Juvenile Manchurian Trout (Brachymystax lenok)

CHANG Jie, NIU Huaxin, HU Zongfu, YU Jianhua, Li Shuguo

doi:10.3969/j.issn.1006-267x.2017.11.030

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 11: 4085 - 4092

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.030

Molecular Nutrition

Requirement of Dietary Vitamin E for Juvenile Manchurian Trout (Brachymystax lenok)

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College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China

Received date: 2017-05-09

Online published: 2017-10-31

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Abstract

This study was conducted to investigate the effects of dietary different levels of vitamin E on growth performance, serum biochemical indicators and vitamin E accumulation in body, and to determine the vitamin E requirements of manchurian trout (Brachymystax lenok). A total of 600 juvenile manchurian trout with an initial body weight of (40.2±3.6) g were randomly divided into 6 groups with 3 replicates per group and 15 fish per replicate. The fish were fed six isonitrogenous and isoenergetic diets containing different levels (measured values were 16.6, 64.9, 165.5, 316.2, 615.5 and 1214.7 mg/kg, respectively) of vitamin E. The experiment lasted for 120 days. The results showed as follows:1) the final weight (FW), weight gain rate (WGR) and special growth rate (SGR) of manchurian trout were firstly increased and then decreased with dietary vitamin E level increasing. The FW of manchurian trout in 165.5 mg/kg group was significantly higher than that in other groups (P<0.05). The WGR and SGR of manchurian trout in 165.5 mg/kg group were significantly higher than those in 16.6 and 1 214.7 mg/kg groups (P<0.05). There were no significant differences in feed intake rate, feed conversion rate and protein efficiency rate of manchurian trout among all groups (P>0.05). 2) With the dietary vitamin E level increasing, the serum triglyceride (TG) and total cholesterol (TC) contents were gradually decreased, which in 615.5 and 1 214.5 mg/kg groups were significantly lower than those in the other groups (P<0.05). With the dietary vitamin E level increasing, the serum high density lipoprotein cholesterol (HDL-C) content firstly increased and then decreased, which in 165.5 mg/kg group was significantly higher than that in other groups (P<0.05), while which in 64.9 and 615.5 mg/kg groups was significantly higher than that in 16.6, 615.5 and 1 214.5 mg/kg groups (P<0.05). The serum low density lipoprotein cholesterol (LDL-C) content had the opposite trend with HDL-C content, which in 165.5 and 316.2 mg/kg groups was significantly lower than that in other groups (P<0.05). There were no significant differences in the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum among all groups (P>0.05). 3) With the dietary vitamin E level increasing, the liver vitamin E accumulation was significantly increased (P<0.05) except that in 15.5 and 1 214.5 mg/kg groups (P>0.05). The muscle vitamin E accumulation was increased with the dietary vitamin E level increasing, which constant on a level when dietary vitamin E level exceeded 165.5 mg/kg, and the muscle vitamin E accumulation in 165.5, 316.2, 615.5 and 1 214.5 mg/kg groups was significantly higher than that in 16.6 and 64.9 mg/kg groups (P<0.05). Therefore, dietary appropriate vitamin E supplemental level can improve the growth performance and serum biochemical indicators of manchurian trout, and increase the accumulation of vitamin E in liver and muscle. According to the broken-line models, WGR and muscle vitamin E accumulation are used as evaluation indexes, the requirement of vitamin E is 145.87 and 180.98 mg/kg, respectively.

Key words： Brachymystax lenok; vitamin E; growth performance; serum biochemical indicators; requirement

Cite this article

CHANG Jie, NIU Huaxin, HU Zongfu, YU Jianhua, Li Shuguo . Requirement of Dietary Vitamin E for Juvenile Manchurian Trout (Brachymystax lenok)[J]. Chinese Journal of Animal Nutrition, 2017 , 29(11) : 4085 -4092 . DOI: 10.3969/j.issn.1006-267x.2017.11.030

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