Achyranthes bidentata Polysaccharides:Regulation on Immunological Stress in Piglet Intestinal Epithelial Cells and Its Action Mechanism

WANG Xiong, LI Mengwei, MA Jie, CHEN Qinghua

doi:10.3969/j.issn.1006-267x.2017.11.034

2017 , Vol. 29 >Issue 11: 4116 - 4122

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.034

Molecular Nutrition

Achyranthes bidentata Polysaccharides:Regulation on Immunological Stress in Piglet Intestinal Epithelial Cells and Its Action Mechanism

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1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China

Received date: 2017-05-11

Online published: 2017-10-31

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Abstract

The purpose of this research was to investigate the effects of Achyranthes bidentata polysaccharides (ABPS) on secretions and expressions of proinflammatory cytokines in piglets jejunum epithelial cells (IPEC-J2) under the immunological stress of lipopolysaccharide (LPS), and to explore the possible mechanism of ABPS to regulate the immune stress of IPEC-J2. The 4th and 5th passages of IPEC-J2 were selected and cultured with 0 (control), 300, 600, 900 and 1 200 μg/mL ABPS, respectively, and both with 10 μg/mL LPS. Each group had 12 replicates with 1proe as 1 replicate. After cultured for 72 h, secretions of proinflammatory cytokines[interleukin (IL-1), interleukin 6 (IL-6), interleukin 8 (IL-8) and tumor necrosis factor α (TNF-α)] were detected by enzyme linked immunosorbent assay (ELISA) method, expression levels of Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB) mRNAs were detected by real time quantitive PCR, and expression levels of TLR4, NF-κB and phosphorylated nuclear factor κB (p-NF-κB) proteins were detected by Western blot method. The results showed as follows:compared with control group, secretions of IL-1, IL-6, IL-8 and TNF-α were significantly decreased in 300, 600, 900 and 1 200 μg/mL ABPS groups (P<0.05); 300 μg/mL ABPS group significantly reduced p-NF-κB protein expression level (P<0.05); 900 and 1 200 μg/mL ABPS group significantly reduced the expression levels of NF-κB and TLR4 mRNAs, and NF-κB protein (P<0.05). In conclusion, ABPS control the release of proinflammatory cytokines and relieve immune stress through TLR4/NF-κB signal transduction pathway, low concentration of ABPS reduce immune stress by inhibiting NF-κB phosphorylation process, high concentration of ABPS alleviate immune stress by inhibiting TLR4 mRNA, NF-κB mRNA and NF-κB protein expressions.

Key words： Achyranthes bidentata polysaccharides; lipopolysaccharide; proinflammatory cytokines; Toll-like receptor 4; nuclear factor κB

Cite this article

WANG Xiong, LI Mengwei, MA Jie, CHEN Qinghua . Achyranthes bidentata Polysaccharides:Regulation on Immunological Stress in Piglet Intestinal Epithelial Cells and Its Action Mechanism[J]. Chinese Journal of Animal Nutrition, 2017 , 29(11) : 4116 -4122 . DOI: 10.3969/j.issn.1006-267x.2017.11.034

References

[1] ALIZADEH A,AKBARI P,DIFILIPPO E,et al.The piglet as a model for studying dietary components in infant diets:effects of galacto-oligosaccharides on intestinal functions[J].British Journal of Nutrition,2015,115(4):605-618.

[2] PETERSON L W,ARTIS D.Intestinal epithelial cells:regulators of barrier function and immune homeostasis[J].Nature Reviews Immunology,2014,14(3):141-153.

[3] 黄志清,陈小玲,陈代文,等.长链n-3多不饱和脂肪酸对肠上皮细胞促炎细胞因子基因mRNA表达的影响[J].动物营养学报,2012,24(7):1384-1388.

[4] 孙丽,夏日炜,殷学梅,等.LPS诱导条件下猪小肠上皮细胞TLR4及其信号通路基因表达变化分析[J].畜牧兽医学报,2015,46(7):1095-1101.

[5] 邹云,谢红兵,贺建华.中草药多糖的免疫调节作用及其受体的研究[J].中国饲料,2013(5):22-25.

[6] TAN F,DENG J.Analysis of the constituents and antisenile function of Achyranthes bidentata polysaccharides[J].Acta Botanica Sinica,2002,44(7):795-798.

[7] 张文俊.牛膝多糖对脂多糖应激断奶仔猪生长性能、免疫功能和肠道功能的影响[D].硕士学位论文.长沙:湖南农业大学,2011.

[8] 秦文雅.牛膝多糖对免疫应激仔猪肠道的影响及其作用机理[D].硕士学位论文.长沙:湖南农业大学,2012.

[9] 袁媛,孙梅.黄芪多糖对LPS损伤小肠上皮细胞的保护作用[J].世界华人消化杂志,2008,16(1):15-19.

[10] BROSNAHAN A J,BROWN D R.Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations[J].Veterinary Microbiology,2012,156(3/4):229-237.

[11] 张永刚,DAVIN R.猪小肠上皮细胞作用的新发现[J].中国畜牧杂志,2015,51(8):57-60.

[12] LIU F N,LI G H,KE W,et al.Porcine small intestinal epithelial cell line (IPEC-J2) of rotavirus infection as a new model for the study of innate immune responses to rotaviruses and probiotics[J].Viral Immunology,2010,23(2):135-149.

[13] ARCE C,RAMÍREZ-BOO M,LUCENA C,et al.Innate immune activation of swine intestinal epithelial cell lines (IPEC-J2 and IPI-2I) in response to LPS from Salmonella typhimurium[J].Comparative Immunology,Microbiology and Infectious Diseases,2010,33(2):161-174.

[14] YANG F J,WANG A N,ZENG X F,et al.Lactobacillus reuteri I5007 modulates tight junction protein expression in IPEC-J2 cells with LPS stimulation and in newborn piglets under normal conditions[J].BMC Microbiology,2015,15:32.

[15] 陈清华,刘祝英,贺建华.牛膝多糖对仔猪淋巴细胞增殖作用和细胞因子分泌量的影响[J].动物营养学报,2008,20(6):712-716.

[16] CHEN Q H,LIU Z Y,HE J H,et al.Achyranthes bidentata polysaccharide enhances growth performance and health status in weaned piglets[J].Food and Agricultural Immunology,2011,22(1):17-29.

[17] 王红权,唐德约,赵玉蓉,等.牛膝多糖对草鱼免疫和抗氧化功能的影响[J].水生生物学报,2013,37(2):351-357.

[18] 朱惠玲,刘玉兰,郭广伦,等.牛膝多糖对免疫应激仔猪生长性能、炎性介质和内分泌激素的影响[J].中国畜牧杂志,2011,47(5):48-51.

[19] 徐小娟.山慈菇多糖、牛膝多糖对H22小鼠肝癌抑制作用及其抗肿瘤机制研究[D].硕士学位论文.长沙:湖南农业大学,2015.

[20] 蔡炳冈,朱进,汪茂荣.Toll样受体4信号通路研究进展[J].医学研究生学报,2015(11):1228-1232.

[21] PÅLSSON-MCDERMOTT E M,O'NEILL L A.Signal transduction by the lipopolysaccharide receptor,Toll-like receptor-4[J].Immunology,2004,113(2):153-162.

[22] LIU S F,MALIK A B.NF-kappa B activation as a pathological mechanism of septic shock and inflammation[J].American Journal of Physiology-Lung Cellular and Molecular Physiology,2006,290(4):L622-L645.

[23] 张彬,肖献忠,张文辉,等.核仁素对LPS诱导的白细胞介素1β释放的影响[J].中国病理生理杂志,2010,26(9):1796-1800.

[24] 陈金龙,张月巧,袁娅,等.植物多糖通过NF-κB信号通路对巨噬细胞的免疫调节作用研究进展[J].食品科学,2015,36(23):288-294.

[25] 武剑.柴胡多糖对巨噬细胞免疫功能的调节及对TLR4信号通路的影响[D].硕士学位论文.上海:复旦大学,2012.

[26] 刘瑞.黄芪多糖影响雏鸡免疫器官TLR4信号转导通路的研究[D].硕士学位论文.哈尔滨:东北农业大学,2015.

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