Molecular Nutrition

Metagenomic Analysis of Microorganisms in Rumen of Haizi Buffalo

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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2017-05-04

  Online published: 2017-10-31

Abstract

In order to investigate microorganism composition and lignocellulolytic enzymes in rumen of Haizi buffalo, rumen fluid sample of Haizi buffalo (about 2.5 years of age, average body weight was 493 kg) was surveyed using metagenomic high-throughput sequencing. The results showed as follws:a total of 77 283 638 reads were obtained, which were assembled into 744 712 scaffolds. And then, a total of 827 044 genes were predicted by prodigal analysis. Many microorganisms involved in lignocellulose degradation were identified by gene annotation, such as Ruminococcus flavefaciens, Ruminococcus albus, Fibrobacter succinogenes and Prevotella ruminicola. We identified potential 38 011 genes encoding lignocellulolytic enzymes including glycoside hydrolase (GH) (17 877 genes), glycosyl transferase (GT) (8 637 genes), carbohydrate binding module (CBM) (4 693 genes), carbohydrate esterase (CE) (4 214 genes), polysaccharide lyase (PE) (1 296 genes), and auxiliary activity (AA) (934 genes). Among GH genes, most genes belonged to GH2, GH43, GH97 and GH3 families, and a higher abundance of oligosaccharide degrading enzymes in buffalo rumen metagenome was identified. Besides that, we also predicted that some genes could encode the cellulosome component. Compared with GH genes in the gastrointestinal metagenome of other species, the proportions of cellulose, hemicellulase and debranching enzyme were similar between Haizi buffalo and dairy cow. These results demonstrate that buffalo rumen is considerably enriched in functional microorganisms and enzymes involved in lignocellulose degradation with great prospects to obtain enzyme genes that may be applied in the industry.

Cite this article

ZHANG Huimin, XIA Hailei, HUANG Qiang, XU Xin, MAO Yongjiang, CEN Ning, YANG Zhangping . Metagenomic Analysis of Microorganisms in Rumen of Haizi Buffalo[J]. Chinese Journal of Animal Nutrition, 2017 , 29(11) : 4151 -4161 . DOI: 10.3969/j.issn.1006-267x.2017.11.039

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