Effects of Yeast Hydrolysate on Growth Performance, Intestinal Morphology and Serum Nonspecific Immune Enzyme Activities of Litopenaeus vannamei

XIONG Jia, YUAN Ye, LU You, MA Hongna, ZHOU Qicun, LIANG Chao, SONG Hongbin, ZOU Qing

doi:10.3969/j.issn.1006-267x.2017.11.045

2017 , Vol. 29 >Issue 11: 4207 - 4215

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.11.045

Molecular Nutrition

Effects of Yeast Hydrolysate on Growth Performance, Intestinal Morphology and Serum Nonspecific Immune Enzyme Activities of Litopenaeus vannamei

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1. Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. Guangdong Hinabiotech Co., Ltd., Guangzhou 510275, China

Received date: 2017-04-10

Online published: 2017-10-31

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Abstract

This experiment was conducted to estimate the effects of dietary supplemental levels of yeast hydrolysate on growth performance, intestinal morphology and serum nonspecific immune enzyme activities of Litopenaeus vannamei. A total of 640 juvenile Litopenaeus vannamei with an initial average body weight of (0.40±0.01) g were randomly divided into four groups with four replicates and 40 Litopenaeus vannamei per replicate. Four isonitrogenous and isolipidic practical diets (42% crude protein and 8% crude lipid) were prepared by adding 0 (control group), 0.5%, 1.0% and 2.0% yeast hydrolysate in a basal diet, respectively. The experiment lasted for 8 weeks. The results showed as follows:1) there were no significant differences in survival rate (SR), weight gain rate (WGR) and specific growth rate (SGR) between experimental groups and control group (P>0.05). 1.0% supplemental group had significantly higher WGR and SGR than 2.0% supplemental group (P<0.05), and significantly lower feed conversion ratio (FCR) than 2.0% supplemental group (P<0.05). Hepatopancreas somatic index (HSI) in 1.0% supplemental group was significantly higher than that in control group (P<0.05), but condition factor (CF) was not significantly affected by yeast hydrolysate supplementation (P>0.05). 2) Dietary supplemental level of yeast hydrolysate had no significant influences on dry matter, crude protein, crude lipid and ash contents of whole shrimp and shrimp muscle (P>0.05). 3) Fold width, microvillus height and enterocyte height of shrimp intestine were not significantly affected by dietary supplementation of yeast hydrolysate (P>0.05). 2.0% supplemental group had a significantly higher fold height in intestine than in 0.5% supplemental group (P<0.05). 4) Compared with control group, serum activities of phenoloxidase (PO) and lysozyme (LZM) of shrimp were significantly increased by dietary supplementation of 1.0% yeast hydrolysate (P<0.05), and serum nitric oxidesynthase (NOS) activity reached the maximum in 1.0% supplemental group. In conclusion, dietary supplementation of 1.0% yeast hydrolysate can significantly increase serum nonspecific immune enzyme activities of Litopenaeus vannamei, and will not exert negatively affect growth performance and intestinal morphology health of Litopenaeus vannamei.

Key words： yeast hydrolysate; Litopenaeus vannamei; growth performance; intestinal morphology; immune enzyme

Cite this article

XIONG Jia, YUAN Ye, LU You, MA Hongna, ZHOU Qicun, LIANG Chao, SONG Hongbin, ZOU Qing . Effects of Yeast Hydrolysate on Growth Performance, Intestinal Morphology and Serum Nonspecific Immune Enzyme Activities of Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 2017 , 29(11) : 4207 -4215 . DOI: 10.3969/j.issn.1006-267x.2017.11.045

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