The objective of this experiment was to research the appropriate level of lysine in the diet for Apis mellifera ligustica worker bee larvae, and to provide the theoretical basis for the study of lysine requirement of Apis mellifera ligustica worker bee in larvae stage. A total of 1 200 one-day-old Apis mellifera ligustica worker bee larvae were randomly divided into 5 groups with 5 replicates per group and 48 larvae per replicate. The worker bee larvae in 5 groups were fed diets with the measured level of lysine was 6.08 (control), 11.08, 16.08, 21.08 and 26.08 mg/g until emergence, respectively. The growth indexes, such as pupation rate (6-day-old), total protein content in whole body (6-day-old), eclosion rate (21-day-old), and the hemolymph biochemical parameters and immune related indicators (6-day-old) of worker bee larvae were measured to certain age, respectively. The results showed as follows:1) the pupation rate and eclosion rate of worker bee larvae in 11.08 mg/g lysine group were extremely significantly higher than those in other groups (P<0.01). 2) Compared with the control group, the total protein content in whole body and the free lysine content in hemolymph of 6-day-old worker bee larvae in 11.08 to 26.08 mg/g lysine groups were significantly increased (P<0.05), and the highest values of them were found in 26.08 mg/g lysine group. 3) The hemolymph triglyceride content of 6-day-old worker bee larvae was decreased firstly and then increased with dietary lysine level increasing, and the lowest value was found in 16.08 mg/g lysine group which was extremely significantly lower than that in other groups (P<0.01). 4) The haemolymph lysozyme activity of 6-day-old worker bee larvae was significantly higher than that of control group (P<0.05), but with dietary lysine level was increased from 21.08 to 26.08 mg/g, the haemolymph lysozyme activity decreased rapidly, and it in 26.08 mg/g lysine group was significantly lower than that in other groups (P<0.01). 5) When dietary lysine level was 11.08 mg/g, the relative expression level of lysozyme gene of 6-day-old worker bee larvae was the highest, which was significantly higher than that of the other lysine levels (P<0.05). Compared with the control group, the relative expression level of defensin 1 gene of 6-day-old worker bee larvae in 11.08 to 16.08 mg/g lysine groups was significantly increased (P<0.05). In conclusion, lysine can promote the growth of Apis mellifera ligustica worker bee larvae, promote the deposition of protein and lysine, increase the rate of eclosion, and regulate the lipid metabolism and the immunity of larvae to some extent. Based on the above indexes, it is recommended that the appropriate lysine level in the diet of Apis mellifera ligustica worker bee larvae is 11.08 to 16.08 mg/g.