A Comparative Study:Effects of 80% L-lysine&middot;H2SO4 and 98% L-lysine&middot;HCl on Growth Performance, Digestion and Absorption Capacities and Growth Development of Digestive Organs of Young Grass Carp (Ctenopharyngodon idella)

HU Kai, SU Yuening, FENG Lin, LIU Yang, JIANG Weidan, WU Pei, JIANG Jun, ZHOU Xiaoqiu

doi:10.3969/j.issn.1006-267x.2017.12.018

2017 , Vol. 29 >Issue 12: 4372 - 4385

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.12.018

Molecular Nutrition

A Comparative Study:Effects of 80% L-lysine·H₂SO₄ and 98% L-lysine·HCl on Growth Performance, Digestion and Absorption Capacities and Growth Development of Digestive Organs of Young Grass Carp (Ctenopharyngodon idella)

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1. Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China;
2. Department of Animal and Veterinary Science, Chengdu Agricultural College, Chengdu 611130, China;
3. Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;
4. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2017-05-05

Online published: 2017-12-04

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Abstract

This study aimed to compare the effects of 80% L-lysine·H₂SO₄ (80-lysine) and 98% L-lysine·HCl (98-lysine) on growth performance, digestion and absorption capacities and growth development of digestive organs of young grass carp (Ctenopharyngodon idella), to explore the biological values of 98-lysine and 98-lysine, and to determine the optimum dietary lysine content using 80-lysine as the supplemental form of lysine. A total of 540 grass carp with an initial body weight about 275.80 g were randomly divided into 6 groups with 3 replicates each and 30 fish per replicate. Fish in 6 groups were fed adding 80-lysine diets containing 0.8% (basal diet), 1.0%, 1.2%, 1.4% and 1.6% lysine and adding 98-lysine diet containing 1.2% lysine for 60 d, respectively. The results showed as follows:compared with the basal diet, dietary lysine content reached 1.2% by adding suitable level of 80-lysine could significantly increase the weight gain rate (WGR), specific growth rate (SGR), feed intake (FI), the activities of lipase, amylase in total intestine, the activities of glutamic oxalacetic transaminase (GOT) and glutamate pyruvate transaminase (GPT) in hepatopancreas, the activities of alkaline phosphatase (AKP) and creatine kinase (CK)in proximal intestine (PI), mid intestine (MI) and distal intestine (DI), hepatosomatic index (HSI), intestosomatic index (ISI) and the fold height of PI and DI (P<0.05), and significantly decrease the activities of GOT and GPT in serum (P<0.05); the effects of 80-lysine on these parameters were better than 98-lysine (P<0.05). Moreover, compared with the basal diet, dietary lysine content reached 1.2% by adding suitable level of 80-lysine could significantly increase the feed efficiency (FE), total intestine trypsin activity, the activities of Na⁺, K⁺-ATPase and γ-glutamyltransferase (γ-GT) in PI, MI and DI, intestinal length, relative gut length (RGL) and fold height in MI (P<0.05); however, compared with 98-lysine, no significant effects of 80-lysine were observed on these parameters (P>0.05). In conclusion, 80-lysine is more effective than 98-lysine for improving growth, and digestion and absorption capacities of young grass carp. Using 80-lysine as the supplemental form of lysine, the optimum dietary lysine content for young grass carp (276 to 667 g) base on SGR and FE is 1.31% (4.68% of dietary protein) and 1.27% (4.54% of dietary protein), respectively.

Key words： 80% L-lysine·H₂SO₄; 98% L-lysine·HCl; grass carp (Ctenopharyngodon idella); growth performance; digestion and absorption capacities

Cite this article

HU Kai, SU Yuening, FENG Lin, LIU Yang, JIANG Weidan, WU Pei, JIANG Jun, ZHOU Xiaoqiu . A Comparative Study:Effects of 80% L-lysine·H₂SO₄ and 98% L-lysine·HCl on Growth Performance, Digestion and Absorption Capacities and Growth Development of Digestive Organs of Young Grass Carp (Ctenopharyngodon idella)[J]. Chinese Journal of Animal Nutrition, 2017 , 29(12) : 4372 -4385 . DOI: 10.3969/j.issn.1006-267x.2017.12.018

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