Resveratrol Induces Apoptosis of Bovine Adipocytes through Activiate of Sirtuin Type 1/Adenosine Monophosphate Activated Protein Kinase Signaling Pathway

CHENG Haijian, YOU Wei, JIN Qing, LIU Yifan, WAN Fachun, SONG Enliang, LIU Guifen, TAN Xiuwen, ZHANG Xianglun, LIU Xiaomu

doi:10.3969/j.issn.1006-267x.2017.12.020

2017 , Vol. 29 >Issue 12: 4398 - 4407

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.12.020

Molecular Nutrition

Resveratrol Induces Apoptosis of Bovine Adipocytes through Activiate of Sirtuin Type 1/Adenosine Monophosphate Activated Protein Kinase Signaling Pathway

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1. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China;
2. Shandong Key Lab of Animal Disease Control and Breeding, Jinan 250100, China

Received date: 2017-06-11

Online published: 2017-12-04

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Abstract

The aim of this study was to explore the effect of plant extracts resveratrol (RES) on the bovine adipocytes apoptosis ratio and key factors mRNA and protein expression in sirtuin type 1 (SIRTl)/adenosine monophosphate activated protein kinase (AMPK) signaling pathway. Bovine subcutaneous preadipocytes of 18-month-old Luxi yellow cattle were selected, on day 0 of cell differentiation, cells were treated with culture medium with 0 (control), 100, 200 and 400 μmol/L RES for 48 h, there were three replicates in each group. Hoechst 33342 staining was used to detect morphological changes of adipocytes apoptosis, the flow cytometry was used to detect the apoptosis ratio, quantitative real-time PCR (qPCR) and Western-blot were selected to detect the gene and protein expressions of SIRTl/AMPK signaling pathway related genes such as SIRT1, AMPKα, forkhead box protein O1 (FoxO1), B cell lymphoma/lewkmia-2 (Bcl-2), cystein-asparate protease-3 (caspase-3), Bcl-2 associated X protein (Bax), and oil red O stain was taken to identify the adipocytes. The results showed that compared with the control group, the adipocyte apoptosis rate after treatment with different concentrations of RES was extremely significantly increased (P<0.01); the mRNA expressions of SIRT1, AMPKα, caspase-3 and Bax after treatment with different concentrations of RES were significantly or extremely significantly increased (P<0.05 or P<0.01), but the Bcl-2 mRNA expression was extremely significantly decreased (P<0.01); the protein expressions of SIRT1, AMPKα and caspase-3 after treatment with different concentrations of RES were significantly or extremely significantly increased (P<0.05 or P<0.01); after treatment with 200 and 400 μmol/L RES, the mRNA and protein expressions of FoxO1 were significantly or extremely significantly increased (P<0.05 or P<0.01), the Bax protein expression was extremely significantly increased (P<0.01), the Bcl-2 protein expression was extremely significantly decreased (P<0.01). After treatment with 100 μmol/L RES, the mRNA and protein expressions of FoxO1 and protein expressions of Bcl-2 and Bax had no significant difference compared with control group (P>0.05). In conclusion, the regulation of apoptosis of bovine subcutaneous adipocytes by RES is through stimulation of AMPK/SIRT1 signaling pathway and activation pathway downstream of FoxO1, which provides a theoretical basis for reducing the caccumulation of subcutaneous fat by nutritional regulation techniques.

Key words： SIRT1; AMPK; FoxO1; resveratrol; bovine; adipocytes; apoptosis

Cite this article

CHENG Haijian, YOU Wei, JIN Qing, LIU Yifan, WAN Fachun, SONG Enliang, LIU Guifen, TAN Xiuwen, ZHANG Xianglun, LIU Xiaomu . Resveratrol Induces Apoptosis of Bovine Adipocytes through Activiate of Sirtuin Type 1/Adenosine Monophosphate Activated Protein Kinase Signaling Pathway[J]. Chinese Journal of Animal Nutrition, 2017 , 29(12) : 4398 -4407 . DOI: 10.3969/j.issn.1006-267x.2017.12.020

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