Molecular Nutrition

Effects of Zearalenone on Ovary Index, Distribution and Expression of Progesterone Receptors in Ovaries of Weaned Gilts

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  • College of Animal Science and Technology, Shandong Agricultural University, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an 271018, China

Received date: 2017-06-02

  Online published: 2017-12-04

Abstract

The aims of the present study were to investigate the effects of zearalenone on ovary index, distribution and mRNA relative expression of progesterone receptors in ovaries of weaned gilts. Forty healthy weaned piglets (Duroc×Landrace×Large White) aged of 35 to 38 days were allocated into 4 groups according to the average body weight[(14.01 ±0.86) kg]. Animals were fed individually in a metabolic cage for 35 days. Piglets in the control group were fed a basal diet, and in experimental groups were fed basal diets supplemented with 0.5, 1.0 and 1.5 mg/kg ZEA, respectively. The analyzed ZEA levels in four groups were 0, (0.52±0.07) mg/kg, (1.04±0.03) mg/kg and (1.51±0.13) mg/kg. The results indicated that ovary index showed a linear increase (P<0.01) with the ZEA level increasing in the diet of weaned gilts. The ovary index in 1.0 mg/kg ZEA group was significantly higher than that in 1.5 mg/kg ZEA group (P<0.05), and the ovary index in 1.5 mg/kg ZEA group was significantly higher than that in 0.5 mg/kg ZEA group and the control group (P<0.05). The integrated optical density (IOD) and mRNA relative expression of progesterone receptors in ovaries of weaned gilts showed a linear increase (P<0.01) with the ZEA level increasing in the diet of weaned gilts. The IOD and mRNA relative expression of progesterone receptors in 1.5 mg/kg ZEA group was significantly higher than that in 1.0 mg/kg ZEA group, and the IOD and mRNA relative expression of progesterone receptors in 1.0 mg/kg ZEA group was significantly higher than that in 0.5 mg/kg ZEA group and the control group (P<0.05). The results of immunohistochemistry showed that the immunoreactive substance of progesterone receptors in ovaries was mainly distributed in oocytes and granulosa cells, theca cells and vascular wall cells of primordial follicles and growing follicles. With the increase of ZEA level and the degree of follicular atresia, immunoreactivity was significantly enhanced. There was no significant change in the distribution of immunoreactive substance of progesterone receptors in the ovary, due to the presence of ZEA. Thus it can be seen that ZEA (1.0 to 1.5 mg/kg) in feed changes the development of ovary by up-regulating the expression of progesterone receptors. The levels of progesterone receptors exceed the limit of positive regulation of follicular growth, thus promoting follicular atresia, reducing the ovary index, and then the health of reproductive system is affected.

Cite this article

YANG Lijie, HUANG Libo, LI Simeng, LIU Faxiao, JIANG Shuzhen, YANG Zaibin . Effects of Zearalenone on Ovary Index, Distribution and Expression of Progesterone Receptors in Ovaries of Weaned Gilts[J]. Chinese Journal of Animal Nutrition, 2017 , 29(12) : 4510 -4517 . DOI: 10.3969/j.issn.1006-267x.2017.12.032

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