Effects of Rosa roxburghii Tratt. Extract on Growth Performance, Lipid Metabolism and Heat Shock Protein 70 Gene Expression in Liver of Wanxi White Geese under High Temperature in Summer

LI Mengyun, YAO Guojia, YANG Jianping, JI Xiangbo, LIU Jian, ZHANG Xiaogen

doi:10.3969/j.issn.1006-267x.2017.12.033

2017 , Vol. 29 >Issue 12: 4518 - 4524

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.12.033

Molecular Nutrition

Effects of Rosa roxburghii Tratt. Extract on Growth Performance, Lipid Metabolism and Heat Shock Protein 70 Gene Expression in Liver of Wanxi White Geese under High Temperature in Summer

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1. Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China;
2. Zhengzhou University of Light Industry, Zhengzhou 450002, China

Received date: 2017-05-26

Online published: 2017-12-04

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Abstract

The aim of this study was to investigate the effects of Rosa roxburghii Tratt. (RRT) extract on growth performance, lipid metabolism and heat shock protein 70 (HSP70) gene expression in liver of Wanxi white geese under high temperature in summer. One hundred and fifty 28-day-old Wanxi white geese with the similar body weight were randomly allotted to three groups including control group, trial group 1 and trial group 2, and each group had 5 replicates and each replicate had 10 geese. The geese in control group were fed a basal diet, and geese in trial group 1 and trial group 2 were fed experimental diets which supplemented with 100 and 200 mL/kg RRT extract in the basal diet, respectively. The pre-test period lasted for 7 days, and the experimental period lasted for 14 days. During the experiment, the highest temperature was 37℃, the lowest temperature was 24℃, and the average daily temperature was 31.6℃. After feeding two weeks, one goose in each replicate was selected and slaughtered, and then the blood samples were collected to measure serum lipid metabolism indices and the liver samples were separated to measure liver HSP70 gene relative expression level. The results showed that no significant differences were found in the average daily gain (ADG) and average daily feed intake (ADFI) among three groups (P>0.05), however, the gain/feed (F/G) was significantly improved when diet supplemented with 200 mL/kg RRT extract (P<0.05). Diet supplemented with 100 and 200 mL/kg RRT extract could increase the serum high-density lipoprotein (HDL) content (P<0.05), whereas serum total cholesterol (TC) and triglyceride (TG) contents were not significantly affected by RRT extract supplementation (P>0.05). Compared with the control group, the relative expression level of HSP70 gene in liver of trial group 1 and trial group 2 was extremely significantly increased (P<0.01), and trial group 1 was extremely significantly higher than trial group 2 (P<0.01). From above results, it can be concluded that RRT extract supplemented into the diet of Wanxi white geese can improve the feed conversion rate, regulate lipid metabolism and up-regulate liver HSP70 gene expression, so it has obvious anti-stress effect under high temperature in summer, and the anti-stress effect of 100 mL/kg RRT extract is better than that of 200 mL/kg RRT extract.

Key words： Wanxi white geese; Rosa roxburghii Tratt. extract; growth performance; lipid metabolism; HSP70 gene expression

Cite this article

LI Mengyun, YAO Guojia, YANG Jianping, JI Xiangbo, LIU Jian, ZHANG Xiaogen . Effects of Rosa roxburghii Tratt. Extract on Growth Performance, Lipid Metabolism and Heat Shock Protein 70 Gene Expression in Liver of Wanxi White Geese under High Temperature in Summer[J]. Chinese Journal of Animal Nutrition, 2017 , 29(12) : 4518 -4524 . DOI: 10.3969/j.issn.1006-267x.2017.12.033

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