Molecular Nutrition

Effects of Exogenous Lipase on Growth Performance, Body Composition, Serum Biochemical Indices, Digestive Enzyme Activities and Nutrient Apparent Digestibilities of Lateolabrax japonicas

Expand
  • 1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
    3. Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
    4. Guangzhou Fishtech Co., Ltd., Guangzhou 510640, China

Received date: 2017-05-09

  Online published: 2017-12-04

Abstract

This study was conducted to investigate the effect of exogenous lipase supplementation in low-protein and high-fat diets on growth performance, body composition, serum biochemical indices, digestive enzyme activities and nutrient apparent digestibility of Lateolabrax japonicus. A total of 600 Lateolabrax japonicas with the average body weight of (6.26±0.02) g were randomly allocated into 6 groups with 4 replicates per group and 25 fish per replicate. Fish in the positive control group (G+ group) were fed a normal diet with high-protein and low-lipid (41.76% crude protein and 8.34% ether extract), fish in the control group (G0 group) were fed a low-protein and high-fat diet (37.64% crude protein and 11.15% ether extract), and others in the experimental groups (G100, G200, G400 and G800 groups) were fed the low-protein and high-fat diet supplemented with 100, 200, 400 and 800 mg/kg exogenous lipase, respectively. The experiment lasted for 8 weeks. The results showed as follows:1) compared with the G+ group, the final body weight, weight gain rate, feed intake and hepatopanceas index of G0 group were increased (P>0.05), the viscerosomatic index and intraperitioneal fat index of G0 group were significantly increased (P<0.05), the serum total antioxidant capacity of G0 group was significantly decreased (P<0.05), the apparent digestibilities of dry matter, crude protein, ether extract and gross energy of G0 group were significantly decreased (P<0.05), the apparent digestibilities of C16:1-n9 and C18:3-n3 fatty acids of G0 group were significantly increased (P<0.05). 2) Compared with the G0 group, the final body weight and weight gain rate of experimental groups were decreased (P>0.05); the final body weight and weight gain rate of G800 group were significantly decreased (P<0.05), the feed conversion rate of G800 group was significantly increased (P<0.05), the viscerosomatic index and intraperitioneal fat index of G800 group were decreased (P>0.05), the activities of lipase in mid-intestine and protease in liver of G800 group were decreased (P>0.05), the contents of cholesterol, high density lipoprotein cholesterol and triglyceride in serum of G800 group were decreased (P>0.05); the glutamic pyruvic transaminase activity and malondialdehyde content in serum of G200 group were significantly decreased (P<0.05). In conclusion, dietary lipid can partially spare protein, the low-protein and high-lipid diet significantly increased viscerosomatic index and intraperitioneal fat index, but has no significant effect on growth performance, body composition and digestive enzyme activity of Lateolabrax japonicas. Meanwhile, exogenous lipase supplementation in low-protein and high-fat diets made growth performance of Lateolabrax japonicas declined, had no obvious effects on digestive enzyme activity and serum biochemical indices, the digestibilities of ether extract and partial fatty acids are decreased, but the viscerosomatic index and intraperitioneal fat index are decreased to a certain extent, and improve the antioxidant capacity.

Cite this article

WANG Guoxia, WANG Min, SUN Yuping, HU Junru, HUANG Wenqing, CHENG Xiaoying, QIU Shidian, HUANG Yanhua . Effects of Exogenous Lipase on Growth Performance, Body Composition, Serum Biochemical Indices, Digestive Enzyme Activities and Nutrient Apparent Digestibilities of Lateolabrax japonicas[J]. Chinese Journal of Animal Nutrition, 2017 , 29(12) : 4542 -4553 . DOI: 10.3969/j.issn.1006-267x.2017.12.036

References

[1] LIU Y Z,HE G,WANG Q C,et al.Hydroxyproline supplementation on the performances of high plant protein source based diets in turbot (Scophthalmus maximus L.)[J].Aquaculture,2014,433:476-480.

[2] BENLI A Ç K,KÖKSAL G,ÖZKUL A.Sublethal ammonia exposure of Nile tilapia (Oreochromis niloticus L.):effects on gill,liver and kidney histology[J].Chemosphere,2008,72(9):1355-1358.  

[3] Du Z Y,LIU Y J,TIAN L X,et al.Effect of dietary lipid level on growth,feed utilization and body composition by juvenile grass carp (Ctenopharyngodon idella)[J].Aquaculture Nutrition,2005,11(2):139-146.  

[4] LI D L,FU Z T,DUAN Y Q.Fish-expert:a web-based expert system for fish disease diagnosis[J].Expert Systems with Applications,2002,23(3):311-320.  

[5] KIM K A,GU W,LEE I A,et al.High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the TLR4 signaling pathway[J].PLoS One,2012,7(10):e47713.

[6] Al-MARZOOQI W,LEESON S.Effect of dietary lipase enzyme on gut morphology,gastric motility,and long-term performance of broiler chicks[J].Poultry Science,2000,79(7):956-960.  

[7] 叶慧,雷建平,冯定远,等.不同微生物脂肪酶对黄羽肉鸡生长性能、血清生化指标和胸肌脂肪含量的影响[J].华南农业大学学报,2013,34(3):399-404.

[8] LIU S,FENG L,JIANG W D,et al.Impact of exogenous lipase supplementation on growth,intestinal function,mucosal immune and physical barrier,and related signaling molecules mRNA expression of young grass carp (Ctenopharyngodon idella)[J].Fish & Shellfish Immunology,2016,55:88-105.

[9] KAHVECI D,XU X B.Repeated hydrolysis process is effective for enrichment of omega 3 polyunsaturated fatty acids in salmon oil by Candida rugosa lipase[J].Food Chemistry,2011,129(4):1552-1558.  

[10] OKADA T,MORRISSEY M T.Production of n-3 polyunsaturated fatty acid concentrate from sardine oil by lipase-catalyzed hydrolysis[J].Food Chemistry,2007,103(4):1411-1419.  

[11] LINDBERG A,OLIVECRONA G.Lipase evolution:trout,Xenopus and chicken have lipoprotein lipase and apolipoprotein C-Ⅱ-like activity but lack hepatic lipase-like activity[J].Biochimica et Biophysica Acta (BBA):Lipids and Lipid Metabolism,1995,1255(2):205-211.  

[12] 陆国君,罗红宇,钟明杰.鲈鱼Lateolabrax japonicus(Cuvier)幼鱼对饵料中蛋白质、脂肪、碳水化合物需求量的研究[J].现代渔业信息,2005,20(11):22-23.

[13] 马卿山,丁雪梅,白世平,等.饲粮添加脂肪酶对肉鸡生长性能及养分利用率的影响[J].动物营养学报,2013,25(10):2447-2458.

[14] 龚俊勇,李芳林,单安山,等.日粮中添加脂肪酶对白羽肉鸡生长性能、血液生化指标、腹脂率的影响[J].饲料工业,2015,36(14):41-45.

[15] 高映红,白华毅,王桂瑛,等.脂肪酶对白羽肉鸡生长性能与养分利用的影响[J].饲料工业,2016,37(23):37-41.

[16] 李路胜,徐志祥,冯定远,等.脂肪酶前处理油脂的效果及其对肉鸡生长性能的影响[J].饲料工业,2016,37(14):36-41.

[17] 张丽娜,陈代文,余冰,等.不同能量水平饲粮中添加脂肪酶对断奶仔猪生长性能、养分表观消化率和血清生化指标的影响[J].动物营养学报,2015,27(12):3854-3860.

[18] 郑荣辉,左建军,龚俊勇.脂肪酶对断奶仔猪生长性能、养分利用率及血清生化指标的影响[J].饲料工业,2016,37(6):21-25.

[19] 周俊华,刘桂武,何若钢,等.脂肪酶、脂肪粉对断奶仔猪生长性能、免疫、酶活及激素水平的影响[J].饲料工业,2014,35(S1):77-80.

[20] 戴朝洲,邹伟雄,翁晓辉.脂肪酶在肉鸭上的应用研究[J].饲料与畜牧,2014(5):17-19.

[21] 谷金皇,杨毅,冷向军,等.添加外源脂肪酶对瓦氏黄颡鱼的生长、消化酶及血清生化指标的影响[J].上海海洋大学学报,2010,19(6):798-804.

[22] 杨新文,杨毅,冷向军,等.脂肪酶对南方鲇生长性能、消化酶活性及血液生化指标的影响[J].淡水渔业,2010,40(3):23-27.

[23] DIERICK N A,DECUYPERE J A.Endogenous lipolysis in feedstuffs and compound feeds for pigs:effects of storage time and conditions and lipase and/or emulsifier addition[J].Animal Feed Science and Technology,2002,102(1/2/3/4):53-70.

[24] GUPTA R,GUPTA N,RATHI P.Bacterial 1ipases:an overview of production,purification and biochemical properties[J].Applied Microbiology and Biotechnology,2004,64(6):763-781.  

[25] ZUO R T,AI Q H,MAI K S,et al.Effects of dietary n-3 highly unsaturated fatty acids on growth,nonspecific immunity,expression of some immune related genes and disease resistance of large yellow croaker (Larmichthys crocea) followingnatural infestation of parasites (Cryptocaryon irritans)[J].Fish & Shellfish Immunology,2012,32(2):249-258.  

[26] WOOD L G,SCOTTH A,GARGM L,et al.Innate immune mechanisms linking non-esterified fatty acids and respiratory disease[J].Progress in Lipid Research,2009,48(1):27-43.  

[27] TODOR?EVI? M,KJÆR M A,DJAKOVI ? N,et al.N-3 HUFAs affect fat deposition,susceptibility to oxidative stress,and apoptosis in Atlantic salmon visceral adipose tissue[J].Comparative Biochemistry and Physiology part B:Biochemistry & Molecular Biology,2009,152(2):135-143.  

[28] LUO J B,FENG L,JIANG W D,et al.The impaired intestinal mucosal immune system by valine deficiency for young grass carp (Ctenopharyngodon idella) is associated with decreasing immune status and regulating tight junction proteins transcript abundance in the intestine[J].Fish & Shellfish Immunology,2014,40(1):197-207.  

[29] 王国霞,刘群芳,黄文庆,等.复合酶制剂对黄颡鱼生长性能、血清生化和免疫指标的影响[J].南方水产科学,2013,9(6):84-90.

[30] 黄燕华,王国霞,黄文庆,等.酶制剂对南美白对虾幼虾生长、体组成及非特异免疫的影响[C]//第六届饲料安全与生物技术专业委员会大会暨第三届全国酶制剂在饲料工业中的应用学术与技术研讨会.广州:中国畜牧兽医学会,2009.

[31] 周玉,郭文场,杨振国,等.鱼类血液学指标研究的进展[J].上海水产大学学报,2001,10(2):163-165.

[32] 朱心玲,贾丽珠,张明瑛.草鱼血液学的研究Ⅰ.九项血液常数的周年变化[J].水生生物学报,1985,9(3):248-257.

[33] KURTOVIC I,MARSHALL S N,ZHAO X,et al.Lipases from mammals and fishes[J].Reviews in Fisheries Science,2009,17(1):18-40.  

[34] 李爱杰.水产动物营养与饲料学[M].北京:中国农业出版社,1994.

[35] 施培松.复合酶制剂对草鱼鱼种生长性能及消化酶的影响[D].硕士学位论文.武汉:华中农业大学,2005.

[36] 周小秋.复合酶制剂在鲤鱼饵料中适宜添加量研究[J].水利渔业,2001,21(6):7-8.
Outlines

/