Molecular Nutrition

Study on Liver Injury by Fusarium Toxins Contaminated Diet and Liver Natural Restoration of Weaned Gilts

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  • 1. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271000, China;
    2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2017-06-04

  Online published: 2017-12-04

Abstract

This experiment was conducted to investigate the effects of staged feeding Fusarium toxin contaminated diet on serum and liver biochemical and antioxidant indices and relative expressions of liver inflammatory cytokines interleukin-1β (IL-1β) and interleukin-6 (IL-6) mRNA of weaned gilts. A total of 30 Duroc×Landrace×Large White female gilts at 35 days of age with body weight of (8.45±0.94) kg were randomly divided into 3 groups with 10 replicates per group and 1 pig per replicate. Gilts in control group were fed a basal diet continuously and gilts in Fusarium toxins group were fed a Fusarium toxins contaminated diet (0.90 mg/kg zearalenone, 1.43 mg/kg vomitoxin, 5.85 mg/kg fumitremorgin) continuously, while gilts in natural recovery group were fed the basal diet instead of the Fusarium toxins contaminated diet after 35 days. The adaptation period lasted for 7 days and the formal period lasted for 56 days. The results showed as follows:1) compared with the control group, the liver relative weight of gilts in Fusarium toxins group was significantly increased (P<0.05), the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) in serum were significantly increased (P<0.05), while the contents of total protein (TP) and globulin (GLB) in serum were significantly decreased (P<0.05), the activities of glutathione peroxidase (GSH-Px) in serum and total superoxide dismutase (T-SOD) in serum and liver were significantly decreased (P<0.05), the serum and liver malondialdehyde (MDA) contents were significantly increased (P<0.05), the relative expressions of liver inflammatory cytokines IL-1β and IL-6 mRNA were significantly increased (P<0.05). 2) After stopping supply of Fusarium toxins contaminated diet for 21 days, compared with the Fusarium toxins group, the liver relative weight of gilts in natural recovery group was decreased to some extent (P>0.05), the activities of AST, ALT and ALP in serum were significantly decreased (P<0.05), while the contents of TP and GLB in serum were significantly increased (P<0.05), the serum T-SOD activity was significantly increased (P<0.05), the activities of GSH-Px in serum and liver and T-SOD in liver were increased to some extent (P>0.05), the serum and liver MDA contents were significantly decreased (P<0.05), the relative expressions of liver inflammatory cytokines IL-1β and IL-6 mRNA were significantly decreased (P<0.05). These results indicated that long term feeding Fusarium toxins can induce liver injuries in gilts, and impact the liver antioxidant, protein synthesis and immunologic functions; after changing for the basal diet for 21 d, the injuries of liver in gilts are improved to a certain extent.

Cite this article

CHEN Ningbo, CHEN Xiangxing, YANG Weiren, JIANG Shuzhen, HUANG Libo . Study on Liver Injury by Fusarium Toxins Contaminated Diet and Liver Natural Restoration of Weaned Gilts[J]. Chinese Journal of Animal Nutrition, 2017 , 29(12) : 4570 -4577 . DOI: 10.3969/j.issn.1006-267x.2017.12.039

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