Effects of Manganese Content in Fermentation Substrate on in Vitro Rumen Fermentation of Yaks

LIU Huili, XUE Yanfeng, HAO Lizhuang, LIU Shujie, CHAI Shatuo, ZHANG Xiaowei

doi:10.3969/j.issn.1006-267x.2018.01.013

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 1: 92 - 99

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.01.013

Molecular Nutrition

Effects of Manganese Content in Fermentation Substrate on in Vitro Rumen Fermentation of Yaks

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1. State Key Laboratory of Plateau Ecology and Agriculture, Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Xining 810016, China;
2. Qinghai Plateau Yak Research Center, Xining 810016, China;
3. Academy of Science and Veterinary Medicine of Qinghai University, Xining 810016, China

Received date: 2017-07-06

Online published: 2018-01-04

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Abstract

In order to find manganese requirement of yaks, manganese bisglycinate was used as additive in this experiment to investigate the effects of different contents of manganese in fermentation substrate on in vitro rumen fermentation of yaks. Five manganese contents in fermentation substrate were designed, which were 35.00, 40.00, 50.00, 60.00 and 70.00 mg/kg, respectively. The fermentation lasted for 48 h using in vitro rumen fermentation method. After fermentation, gas production, rumen fermentation characteristics and digestive enzyme activities were measured. The results showed as follows:1) when the content of manganese was 40.0 mg/kg, the contents of ammonia nitrogen, microbial protein, acetic acid, propionic acid, isobutyric acid, butyric acid, isovaloric acid, valeric acid and total volatile fatty acids in fermentation fluid reached the highest, which were 10.60 mg/dL, 3.90 g/L, 47.12 mmol/L, 19.45 mmol/L, 0.35 mmol/L, 5.41 mmol/L, 0.96 mmol/L, 0.50 mmol/L and 72.24 mmol/L, respectively; 2) when the content of manganese was 50.00 mg/kg, lipidase activity in fermentation fluid reached the highest as 0.50 U/mL, and acetic acid/propionic acid in fermentation fluid reached the lowest, as 2.05. Therefore, the manganese content recommended in diet for growing yaks is between 40.00 and 50.00 mg/kg, when using manganese bisglycinate as additive, which is help to rumen fermentation and forage degradation.

Key words： yak; manganese bisglycinate; in vitro rumen fermentation; digestive enzyme activity

Cite this article

LIU Huili, XUE Yanfeng, HAO Lizhuang, LIU Shujie, CHAI Shatuo, ZHANG Xiaowei . Effects of Manganese Content in Fermentation Substrate on in Vitro Rumen Fermentation of Yaks[J]. Chinese Journal of Animal Nutrition, 2018 , 30(1) : 92 -99 . DOI: 10.3969/j.issn.1006-267x.2018.01.013

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