Molecular Nutrition

Effects of Starvation Time on Growth, Biochemical Composition, Enzyme Activities and Antioxidant Indexes of Hemifusus tuba Gmelin

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  • School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2017-06-19

  Online published: 2018-01-04

Abstract

This experiment was conducted to reveal the effects of different starvation time on growth, biochemical composition, enzyme activities and antioxidant indexes of Hemifusus tuba Gmelin. A total of 360 Hemifusus tuba Gmelin with the body weight of (28.33±1.27) g were randomly divided into 6 groups with 3 replicates per group and 20 snails per replicate, and the snails in the 6 groups had different starvation time:0 (control group), 5, 10, 20, 30 and 40 days at the water temperature of 29 to 31℃. The results showed that the survival rate, shell height and shell width were not significant affected by starvation time (P>0.05), and the survival rate of each group was 100%. But the weight was gradually decreased with the starvation time increasing, especially for without feeding for 20 to 40 days, and the weight loss was 12.73% to 21.14%. The moisture and crude protein contents in pedal muscle of Hemifusus tuba Gmelin were not significant affected by starvation time (P>0.05) (except starvation 40 days). The content of ether extract in pedal muscle was gradually decreased along with the increase of starvation time, but the content of ash in pedal muscle was gradually increased. With the starvation time increasing, pedal muscle glycogen content was significantly decreased (P<0.05), and liver glycogen content showed a downward trend which was significantly different among different groups (P<0.05) except groups with 10 and 20 days of starvation and groups with 20 and 30 days of starvation. With the starvation time increasing, the contents of saturated fatty acid and monounsaturated fatty acid in pedal muscle were gradually declined, while the content of unsaturated fatty acid was relatively rose. With the starvation time increasing, the content of fatty acids of even number of carbon atoms was gradually decreased, while the number of fatty acids of odd number of carbon atoms was relatively increased. Compared with the control group, the activities of lipase and protease in hepatopancreas of Hemifusus tuba Gmelin in group with 5 days of starvation were significantly increased (P<0.05), and it in the group with 40 days of starvation was significantly decreased (P<0.05). the activity of amylase in hepatopancreas of Hemifusus tuba Gmelin in the group with 10 days of starvation was significantly higher than that of the control group and the group with 10 days of starvation (P<0.05), and it in the group with 40 days of starvation was significantly lower than that of the control group (P<0.05). The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) in hepatopancreas were rose with the increase of starvation time in 5 to 30 days of starvation time, and were significantly higher than those in the control group (P<0.05), while those in the group with 40 days of starvation were significantly lower than those in the control group (P<0.05). The content of malondialdehyde (MDA) in hepatopancreas was not significantly different among groups less than 30 days of starvation (P>0.05), but it was significantly higher than that in the groups with more than 30 days of starvation (P<0.05). The above results indicated that the Hemifusus tuba Gmelin are extremely insusceptible to hunger, but their biochemical markers have changed dramatically after 30 days of starvation at high temperatures (29 to 31℃), indicating that starvation stress has occurred in their body.

Cite this article

WANG Shuangjian, DING Yuhui, JIANG Maowang, JIANG Xiamin, HAN Qingxi . Effects of Starvation Time on Growth, Biochemical Composition, Enzyme Activities and Antioxidant Indexes of Hemifusus tuba Gmelin[J]. Chinese Journal of Animal Nutrition, 2018 , 30(1) : 138 -146 . DOI: 10.3969/j.issn.1006-267x.2018.01.018

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