The objective of the current study was to investigate the effects of yeast culture of white distiller's grains added in different energy level diets on growth performance, carcass composition and serum biochemical indices of broilers. A total of 350 one-day-old male Arbor Acres (AA) broiler chickens were randomly allotted into 5 groups with 7 replicates of 10 birds each. Chickens in 5 groups were fed a basal diet (group Ⅰ, control), the basal diet supplemented with 0.5% yeast culture of white distiller's grains (group Ⅱ), the basal diet supplemented with 1.0% yeast culture of white distiller's grains (group Ⅲ), the reduced 0.21 MJ/kg metabolizable energy basal diet supplemented with 0.5% yeast culture of white distiller's grains (group Ⅳ), and the reduced 0.42 MJ/kg metabolizable energy basal diet supplemented with 1.0% yeast culture of white distiller's grains (group Ⅴ), respectively. The trial lasted for 42 days. The results showed as follows:1) at 21 days of age, the average body weight of broilers in control group was the lowest, and tended to be significant lower than that in group Ⅲ (P<0.10). The ratio of feed to gain (F/G) of broilers in groups Ⅱ and Ⅳ was partially decreased during 22 to 42 days of age compared with control group (P<0.10), and the F/G of broilers in group Ⅱ was lower than that in control group during 1 to 42 days of age (P>0.05). 2) At 21 days of age, the abdominal fat percentage of broilers in group Ⅳ was significantly lower than that in the control group (P<0.05), while the abdominal fat percentage of broilers in group Ⅴ was numerically decreased at 21 and 42 days of age compared with control group (P>0.05). 3) No significant difference in serum total protein, albumin, uric acid, creatinine, calcium or phosphorus content of broilers was found when diet was supplemented with yeast culture of white distiller's grains (P>0.05). 4) At 21 days of age, serum glutathione peroxidase (GSH-Px) activity in groups Ⅱ and Ⅳ was significantly increased compared with control group in which was the lowest (P>0.05). The serum superoxide dismutase activity of broilers in experimental groups was significantly increased compared with control group (P<0.05), and serum total antioxidant capacity (T-AOC) of broilers in groups Ⅱ and Ⅳ was significantly higher than that in control group (P<0.05). At 42 days of age, T-AOC in experimental groups was significantly increased compared with control group (P<0.01). Overall, dietary 0.5% to 1.0% yeast culture of white distiller's grains can increase body weight gain, improve feed efficiency, decrease the abdominal fat percentage and increase the serum antioxidant ability of broilers.