This experiment was conducted to investigate the effect of Eucommia leaves extract on performance, egg quality, yolk cholesterol content and serum antioxidant indexes of laying hens during late period of laying. Based on a single factor experiment design, a total of 1 200 laying hens (420-day-old) with similar laying rate and body weight were randomly divided into 4 groups with 6 replications per group and 50 hens per replicate. Laying hens in the control group were fed a basal diet, and the others in the experimental groups were fed the basal diets supplemented with 100, 200 and 300 g/t Eucommia leaves extract, respectively. The adaptation period lasted for 1 week and the formal period lasted for 8 weeks. The results showed as follows:1) compared with the control group, the unqualified egg rate and mortality rate of experimental groups were significantly decreased (P<0.05). 2) Compared with the control group, at the 56^th day, the eggshell thickness, eggshell strength of experimental groups were significantly increased (P<0.05), the yolk cholesterol content of experimental groups was significantly decreased (P<0.01). 3) Compared with the control group, the activities of superoxide dismutase and catalase in serum of experimental groups were significantly increased (P<0.01), while the serum malondialdehyde content of experimental groups was significantly decreased (P<0.01). In conclusion, dietary supplemented with Eucommia leaves extract can reduce the unqualified egg rate and mortality rate, increase the eggshell thickness and eggshell strength, reduce the yolk cholesterol content, improve the body antioxidant capacity. The best sepplemented level of Eucommia leaves extract in the basal diet was 300 g/t.