Molecular Nutrition

Screen of Glucosinolate Detox Strain and Its Effects on Fermentation of Rapeseed Meal

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  • Key Laboratory of Feed Biotechnology of Agricultural Ministry, National Research Center of Biological Feed, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2017-07-04

  Online published: 2018-01-04

Abstract

The aim of this study was to screen the bacterial strain for detoxicating rapeseed meal and to investigate its effects on fermentation of rapeseed meal. Fifty-four strains with good growth were screened and isolated from mixed liquid with fermented silage and soil through cultivating in medium containing glucosinolate (GS) as a sole carbon source. Five strains were selected based on the degradation ability of GS with degradation rate greater than 30%. According to re-screening in the solid-state fermentation, strain named A9 showed high degradation rate (23.69%) of GS. Strain A9 has been identified as Lactobacillus farciminis after morphological observation and 16s rDNA sequence analysis. Rapeseed meal was fermented through mixing Lactobacillus farciminis with Bacillus subtilis and Saccharomyces cerevisiate. The results showed that the GS content of rapeseed meal fermented by mixed bacteria decreased from 37.48 to 25.96 μmol/g, and the degradation rate was 30.73%; the crude protein content increased from 38.41% to 45.44% (P<0.05); the protein structure changed, and the molecular weight of protein became smaller, the polypeptide content increased from 0.84% to 2.62% (P<0.05); the rapeseed meal fermented by mixed bacteria was yellow-brown and fluffy with a rich sour aroma, and the total acid content increased from 1.01% to 3.91% (P<0.05). In conclusion, the nutritional value and relish of rapeseed meal fermented by mixed bacteria can efficiently improve.

Cite this article

WU Zhengke, LIU Guohua, CAI Huiyi, CHANG Wenhuan, ZHENG Aijuan, ZHANG Shu . Screen of Glucosinolate Detox Strain and Its Effects on Fermentation of Rapeseed Meal[J]. Chinese Journal of Animal Nutrition, 2018 , 30(1) : 313 -320 . DOI: 10.3969/j.issn.1006-267x.2018.01.037

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