Molecular Nutrition

Effects of Lactobacillus casei on Growth Performance and Intestinal Microbiota of Beijing Black Pigs in Nursery Stage

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  • 1. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China;
    2. Beijing Heiliu Animal Husbandry Science and Technology Co., Ltd., Beijing 102211, China

Received date: 2017-06-29

  Online published: 2018-01-04

Abstract

This experiment was conducted to study the effects of Lactobacillus casei on growth performance and intestinal microflora of Beijing black pigs in nursery stage. A total of 120 weaned piglets for Beijing black pigs with the age of (35±2) days and average body weight of (7.53±0.21) kg were randomly distributed into 3 groups with 4 replicates per group and 10 piglets per replicate, comprising of control group, Lactobacillus casei group and chlortetracycline group. The piglets in the control group were fed a basal diet (without Lactobacillus casei or chlortetracycline), the piglets in the Lactobacillus casei group were fed the basal diet supplemented with living bacteria freeze dry preparation of Lactobacillus casei, and the living bacteria count was 4.0×109 CFU/kg diet, and the piglets in the chlortetracycline group were fed the basal diet supplemented with chlortetracycline premix, and the chlortetracycline content was 75 mg/kg diet. The experiment lasted for 30 d. At the 28th day of the experiment, the fresh feces were collected and used for sequencing of the V3-V4 hypervariable region of the 16S rRNA. The results showed as follows:1) compared with the control group, the average daily gain of Lactobacillus casei group and chlortetracycline group was significantly increased by 12.71% (P<0.05) and 8.58% (P<0.05), the feed/gain was decreased by 7.34% (P<0.05) and 4.52% (P>0.05), respectively. 2) Lactobacillus casei group increased the species richness and diversity of microbiota, as indicated by the ACE index, Chao1 index, Shannon index and Simpson index, compared with the control group and the chlortetracycline group. 3) Seventeen phyla and 206 genera were contained by three groups of feces, and 198, 200 and 197 genera were found in the control group, Lactobacillus casei group and chlortetracycline group, respectively. The results indicate that Lactobacillus casei supplementation in the diet has beneficial effects on the improvement of intestinal microflora structure and growth performance of Beijing black pigs in nursery stage.

Cite this article

WANG Sixin, JI Haifeng, SHI Guohua, LIU Hui, WANG Hongwei, ZHANG Dongyan, WANG Jing, ZHANG Wei, WANG Yamin . Effects of Lactobacillus casei on Growth Performance and Intestinal Microbiota of Beijing Black Pigs in Nursery Stage[J]. Chinese Journal of Animal Nutrition, 2018 , 30(1) : 326 -335 . DOI: 10.3969/j.issn.1006-267x.2018.01.039

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