Effects of Flaxseed Oil on Intestinal Mucosa Structure and Immune Cells of Weaned Piglets after Lipopolysaccharide Challenge

LI Xiangen, TU Zhixiao, WANG Shuhui, WANG Haibo, ZHANG Lin, QIN Qin, WANG Longmei, WANG Xiuying, LIU Yulan, ZHU Huiling

doi:10.3969/j.issn.1006-267x.2018.02.015

2018 , Vol. 30 >Issue 2: 515 - 523

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.02.015

Molecular Nutrition

Effects of Flaxseed Oil on Intestinal Mucosa Structure and Immune Cells of Weaned Piglets after Lipopolysaccharide Challenge

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Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2017-08-21

Online published: 2018-02-02

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Abstract

The purpose of this study was to investigate the effects of flaxseed oil on intestinal mucosa structure and immune cells of weaned piglets after lipopolysaccharide (LPS) challenge. Twenty-four Duroc×Landrace×Yorkshine crossed-bred weaned piglets with average body weight (BW) of (9.15±0.90) kg were randomly divided into four groups with six replicates each and one pig per replicate. The experiment consisted of a 2×2 factorial design, and the main factors included:1) diet treatment (5% flaxseed oil or 5% corn oil); 2) immunological challenge (LPS or physiological saline injection). After being fed diets supplemented with 5% corn oil or 5% flaxseed oil for 21 days, pigs were injected intraperitoneally with 100 μg/kg BW LPS or the same doze of saline half and half. At 4 h postinjection, pigs were anaesthetized and slaughtered, and the intestinal samples were collected for analysis. The results showed as follows:1) LPS challenge significantly decreased villus height in jejunum and ileum as well as crypt depth in jejunum (P<0.05), and significantly increased the lamina propria cell density and the number of neutrophils in jejunum (P<0.05). LPS challenge tended to reduce the number of epithelial lymphocytes (IELs) in jejunum (P=0.080)and the number of goblet cells in ileum (P=0.059). 2) LPS challenge did not significantly affect villus height in jejunum and ileum of pigs in flaxseed oil groups (P>0.05). However, dietary 5% flaxseed oil tended to increase the Peyer's patch cell density in ileum (P=0.064), especially under LPS challenge, significantly reduced the lamina propria cell density in jejunum (P<0.05), and tended to increase the number of IELs in jejunum (P=0.069). These results indicate that 5% flaxseed oil can maintain small intestinal mucosa structure and immune function of weaned piglets after LPS challenge.

Key words： flaxseed oil; weaned piglets; lipopolysaccharide; intestinal mucosa immune cells

Cite this article

LI Xiangen, TU Zhixiao, WANG Shuhui, WANG Haibo, ZHANG Lin, QIN Qin, WANG Longmei, WANG Xiuying, LIU Yulan, ZHU Huiling . Effects of Flaxseed Oil on Intestinal Mucosa Structure and Immune Cells of Weaned Piglets after Lipopolysaccharide Challenge[J]. Chinese Journal of Animal Nutrition, 2018 , 30(2) : 515 -523 . DOI: 10.3969/j.issn.1006-267x.2018.02.015

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