Molecular Nutrition

Bacteria Diversity in Gastrointestinal Tract of a Dead Wild Rhinopithecus roxellanae Analyzed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis Technology

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  • 1. Institute of Animal Microecology, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
    2. Chengdu Zoo, Chengdu 610081, China;
    3. Sichuan Animal Science Institute, Chengdu 610066, China

Received date: 2017-07-14

  Online published: 2018-02-02

Abstract

The aim of this experiment was to evaluate the bacteria diversity in gastrointestinal tract (GIT) of a dead wild Rhinopithecus roxellanae, and to analyze the phylogenetic tree of cloning sequencing bands. The GIT contents of a dead wild Rhinopithecus roxellanae were collected, and the polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) technology with the cloning sequencing, cluster analysis and principal component analysis (PCA) of bands were used to detect the bacterial diversity and to build the phylogenetic tree. The results showed as follows:1) numerous bacteria were obtained from the GIT of wild Rhinopithecus roxellanae. Samples from the stomach, duodenum, jejunum and ileum clustering together, samples from the cecum, colon and rectum clustering together, and samples from the faeces single clustering together. 2) The 18 identified DGGE bands were belong to five phylum, they were Proteobacteria (38.89%), Firmicutes (22.22%), Bacteroidetes (5.56%), Actinobacteria (5.56%), Verrucomicrobia (5.56%) and uncultured bacterium (22.22%). The Proteobacteria and Firmicutes were detected along the GIT. 3) The phylogenetic tree analysis showed that only one of uncultured bacterium was similar to the identified classification of Enterococcus faecalis, while the other three uncultured bacteria had a significant difference with the known branch of the bacteria. This indicated that a large number of flora information was unkowned in the GIT of the wild Rhinopithecus roxellanae. The results suggest that Proteobacteria is predominant in the GIT of a dead wild Rhinopithecus roxellanae, and the bacteria diversity shows a tendency of high-low-high according to the GIT from front to back.

Cite this article

ZENG Yan, NIU Lili, DENG Jiabo, WANG Qiang, YU Jianqiu, LI Yang, ZHOU Mengjia, XU Shuai, LIN Yiceng, ZENG Dong, NI Xueqin . Bacteria Diversity in Gastrointestinal Tract of a Dead Wild Rhinopithecus roxellanae Analyzed by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis Technology[J]. Chinese Journal of Animal Nutrition, 2018 , 30(2) : 649 -657 . DOI: 10.3969/j.issn.1006-267x.2018.02.030

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