Effects of Pyrroloquinoline Quinone Disodium on Performance, Antioxidant Status and Plasma Biochemical Parameters of Laying Hens

WANG Jing, YANG Linlin, ZHANG Haijun, WU Shugeng, QI Guanghai

doi:10.3969/j.issn.1006-267x.2018.02.032

2018 , Vol. 30 >Issue 2: 667 - 677

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.02.032

Molecular Nutrition

Effects of Pyrroloquinoline Quinone Disodium on Performance, Antioxidant Status and Plasma Biochemical Parameters of Laying Hens

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National Engineering Research Center of Biological Feed, Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2017-08-15

Online published: 2018-02-02

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Abstract

The current study aimed to evaluate the biological safety of pyrroloquinoline quinone disodium (PQQ·Na₂) for laying hens and investigated the effects of dietary PQQ·Na₂ suplemental levels on performance, antioxidant status and plasma biochemical parameters. Five hundred and forty 25-week-old Hyline Grey laying hens were randomly divided into 5 groups with each group consisted of 6 replicates of 18 birds each. The laying hens in the 5 groups were fed 5 experimental diets with 0 (control), 0.04, 0.08, 0.12 mg/kg PQQ·Na₂ and 200 mg/kg vitamin E, respectively. All hens were fed the diets for a 1-week adaptation followed by a 24-week trial period. The result showed as follows:1) diet supplemented with 0.04 to 0.12 mg/kg PQQ·Na₂ did not affect the performance of laying hens (P>0.05). 2) Compared with the control group, the Haugh unit was significantly increased by diet supplemented with 0.04 to 0.12 mg/kg PQQ·Na₂ (P<0.05), and the eggshell thickness was significantly increased by diet supplemented with 0.08 mg/kg PQQ·Na₂ at the week 24 of trial (P<0.05). 3) Compared with the control group, the activities of plasma glutathion peroxidase (GSH-Px) (week 12) and total superoxide dismutase (T-SOD) (week 2 and 24) were significantly increased by diet supplemented with 0.08 and 0.12 mg/kg PQQ·Na₂ (P<0.05). 4) Compared with the control group, diet supplemented with PQQ·Na₂ at 0.08 mg/kg significantly decreased the content of heart carbonyl group and significantly increased the hepatic total antioxidant capacity (T-AOC) (P<0.05), and diet supplemented with 0.12 mg/kg PQQ·Na₂ significantly decreased the hepatic malondialdehyde (MDA) content (P<0.05). Above results indicate that the supplementation of PQQ·Na₂ at 0.08 to 0.12 mg/kg in laying hens' diet do not have negative effects, but can enhance the laying hens' antioxidant function. The suitable supplemental level of PQQ·Na₂ in laying hens' diet is 0.08 mg/kg by comprehensive considering of the influence factors.

Key words： pyrroloquinoline quinone disodium; laying hens; antioxidant; plasma biochemical parameters; egg quality

Cite this article

WANG Jing, YANG Linlin, ZHANG Haijun, WU Shugeng, QI Guanghai . Effects of Pyrroloquinoline Quinone Disodium on Performance, Antioxidant Status and Plasma Biochemical Parameters of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2018 , 30(2) : 667 -677 . DOI: 10.3969/j.issn.1006-267x.2018.02.032

References

[1] TOSHIHIRO T,TOSHIFUMI Y,KIYOHIRO H,et al.The protective effect of pyrroloquinoline quinone and its derivatives against carbon tetrachloride-induced liver injury of rats[J].Journal of Gastroenterology and Hepatology,1993,8(4):342-347.

[2] XIONG X H,ZHAO Y,GE X,et al.Production and radioprotective effects of pyrroloquinoline quinone[J].International Journal of Molecular Science,2011,12(12):8913-8923.

[3] XU F,YU H X,LIU J Y,et al.Pyrroloquinoline quinone inhibits oxygen/glucose deprivation-induced apoptosis by activating the PI3K/AKT pathway in cardiomyocytes[J].Molecular and Cellular Biochemistry,2014,386(1/2):107-115.

[4] ZHANG Q,DING M,CAO Z,et al.Pyrroloquinoline quinine protects rat brain cortex against acute glutamate-induced neurotoxicity[J].Neurochemical Research,2013,38(8):1661-1671.

[5] ZHOU X Q,CHEN Q C,HU X D,et al.Pyrroloquinoline quinone prevents MK-801-induced stereotypical behavior and cognitive deficits in mice[J].Behavioural Brain Research,2014,258:153-159.

[6] 徐磊.日粮中添加吡咯喹啉醌对产蛋鸡生产性能和抗氧化机能的影响[D].硕士学位论文.北京:中国农业科学院,2012:7-37.

[7] 赵芹,张海军,武书庚,等.吡咯喹啉醌对高能低蛋白质饲粮蛋鸡生产性能、蛋品质、血浆脂质代谢及抗氧化能力的影响[J].动物营养学报,2014,26(4):885-892.

[8] WANG J,ZHANG H J,XU L,et al.Dietary supplementation of pyrroloquinoline quinone disodium protects against oxidative stress and liver damage in laying hens fed an oxidized sunflower oil-added diet[J].Animal,2016,10(7):1129-1136.

[9] 赵芹,张海军,武书庚,等.吡咯喹啉醌对脂肪肝蛋鸡肝损伤的保护作用机制[J].动物营养学报,2014,26(4):651-658.

[10] 孙丽敏.吡咯喹啉醌钠(PQQ·Na₂)对蛋鸡生产性能和脂质代谢的影响[D].硕士学位论文.北京:中国农业科学院,2015:16-17.

[11] KILLGORE J,SMIDT C,DUICH L,et al.Nutritional importance of pyrroloquinoline quinine[J].Science,1989,245(4920):850-852.

[12] STEINBERG F M,GERSHWIN M E,RUCKER R B.Dietary pyrroloquinoline quinone:growth and immune response in BALB/c Mice[J].Journal of Nutrition,1994,124(5):744-753.

[13] STEINBERG F M,STITES T E,ANDERSON P,et al.Pyrroloquinoline quinone improves growth and reproductive performance in mice fed chemically defined diets[J].Experimental Biology and Medicine,2003,228(2):160-166.

[14] NOJI N,NAKAMURA T,KITAHATA N,et al.Simple and sensitive method for pyrroloquinoline quinone (PQQ) analysis in various foods using liquid chromatography/electrospray-ionization tandem mass spectrometry[J].Journal of Agricultural Food and Chemistry,2007,55(18):7258-7263.

[15] 徐磊,张海军,武书庚,等.吡咯喹啉醌对蛋鸡生产性能、蛋品质及抗氧化功能的影响[J].动物营养学报,2011,23(8):1370-1377.

[16] TOUSSANT M J,LATSHAW J D.Ovomucin content and composition in chicken eggs with different interior quality[J].Journal of the Science of Food and Agriculture,1999,79(12):1666-1670.

[17] RABIE M H,SZILÁGYI M,GIPPERT T.Effects of dietary L-carnitine on the performance and egg quality of laying hens from 65-73 weeks of age[J].British Journal of Nutrition,1997,78(4):615-623.

[18] AUSTIC R E.Role of the shell gland in determination of albumen quality[J].Poultry Science,1977,56(1):202-210.

[19] MUOIO D M,KOVES T R.Skeletal muscle adaptation to fatty acid depends on coordinated actions of the PPARs and PGC-1α:implications for metabolic disease[J].Applied Physiology,Nutrition,and Metabolism,2007,32(5):874-883.

[20] STITES T,STORMS D,BAUERLY K,et al.Pyrroloquinoline quinine modulates mitochondrial quantity and function in mice[J].Journal of Nutrition,2006,136(2):390-396.

[21] SAHIN K,AKDEMIR F,ORHAN C,et al.Effects of dietary resveratrol supplementation on egg production and antioxidant status[J].Poultry Science,2010,89(6):1190-1198.

[22] FRANCHINI A,SIRRI F,TALLARICO N,et al.Oxidative stability and sensory and functional properties of eggs from laying hens fed supranutritional doses of vitamins E and C[J].Poultry Science,2002,81(11):1744-1750.

[23] AIZENMAN E,HARTNETT K A,ZHONG C,et al.Interaction of the putative essential nutrient pyrroloquinoline quinone with the N-methyl-D-aspartate receptor redox modulatory site[J].The Journal of Neuroscience,1992,12(6):2362-2369.

[24] KHAIRNAR N P,MISRA H S,APTE S K.Pyrroloquinoline-quinone synthesized in Escherichia coli by pyrroloquinoline-quinone synthase of Deinococcus radiodurans plays a role beyond mineral phosphate solubilization[J].Biochemical and Biophysical Research Communications,2003,312(2):303-308.

[25] ZHANG P,XU Y P,SUN J X,et al.Protection of pyrroloquinoline quinone against methylmercury-induced neurotoxicity via reducing oxidative stress[J].Free Radical Research,2009,43(3):224-233.

[26] AKAIKE T,SATO K,KOHNO M,et al.PQQ as a generator and a scavenger of oxygen radicals determination with ESR spectroscopy using a spin trap agent[C]//FUKUI T,KAGAMIYAMA H,SODA K,et al.Enzymes dependent on pyridoxal phosphate and other carbonyl compounds as confactors.New York:Pergamon Press,1991:511-513.

[27] 王凤来,张曼夫,陈清明,等.日粮磷和钙磷比例对小型猪(香猪)血清、肠、骨碱性磷酸酶及血清钙磷的影响[J].动物营养学报,2001,13(1):36-42.

[28] REICHMANN K G,CONNOR J K.Influence of dietary calcium and phosphorus on metabolism and production in laying hens[J].British Poultry Science,1977,18(6):633-640.

[29] 张亚男.饲粮锌对产蛋后期蛋鸡蛋壳品质及抗氧化机能的影响[D].硕士学位论文.北京:中国农业科学院,2013:38-39.

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