Effects of Xylooligosaccharide on Growth Performance, Serum Biochemical and Immune Indices and Intestinal Microflora Composition of Juvenile Japanese Seabass (Lateolabrax japonicus)

HU Xiaowei, SHANGGUAN Jinbo, LI Zhongbao, YANG Min, XU Anle

doi:10.3969/j.issn.1006-267x.2018.02.039

2018 , Vol. 30 >Issue 2: 734 - 742

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.02.039

Molecular Nutrition

Effects of Xylooligosaccharide on Growth Performance, Serum Biochemical and Immune Indices and Intestinal Microflora Composition of Juvenile Japanese Seabass (Lateolabrax japonicus)

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1. Fisheries College of Jimei University, Xiamen 361021, China;
2. Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment, Xiamen 361021, China

Received date: 2017-08-17

Online published: 2018-02-02

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Abstract

This study was conducted to determine the effects of xylooligosaccharide (XOS) on growth performance, serum biochemical and immune indices and intestinal microflora composition of juvenile Japanese seabass (Lateolabrax japonicus). A total of 360 juvenile Japanese seabass were randomly assigned to 6 groups with 3 replicates per group and 20 fish per replicate. Fish in the 6 groups were fed the basal diets supplemented with 0 (control group), 200 (D200 group), 400 (D400 group), 600 (D600 group), 800 (D800 group) and 1 000 mg/kg XOS (D1000 group), respectively. The experiment lasted for 45 days. The results showed as follows:1) compared with the control group, the weight gain rate (WGR) and specific growth rate (SGR) of D200, D400 and D600 groups were increased (P>0.05), but the WGR and SGR of D800 (P>0.05) and D1000 groups (P<0.05) were decreased. 2) Compared with the control group, the serum total protein content of D200, D400, D600, D800 and D1000 groups was significantly increased (P<0.05), the serum high-density lipoprotein cholesterol content of D400 and D800 groups was significantly increased (P<0.05), the serum total cholesterol content of D200 group was significantly decreased (P<0.05). 3) Compared with the control group, the serum superoxide dismutase activity of D400, D600, D800 and D1000 groups was significantly decreased (P<0.05), the serum peroxidase activity of D200 and D400 groups was significantly decreased (P<0.05), the serum lysozyme activity of D400 and D500 groups was significantly increased (P<0.05), the serum malondialdehyde content of D200 group was decreased (P>0.05). 4) Compared with the control group, the intestinal Salmonella number of D200, D400, D600, D800 and D1000 groups was significantly decreased (P<0.05), the intestinal Escherichia coli number of D200, D400 and D1000 groups was significantly decreased (P<0.05), the intestinal Bifidobacterium number of D200, D400 and D600 groups was significantly increased (P<0.05). In conclusion, comprehensive all the experimental indices, diet supplemented with 350 to 560 mg/kg XOS has best effects on growth performance, immune function and intestinal health of juvenile Japanese seabass.

Key words： Lateolabrax japonicas; growth performance; xylooligosaccharide; non-specific immune; intestinal microflora

Cite this article

HU Xiaowei, SHANGGUAN Jinbo, LI Zhongbao, YANG Min, XU Anle . Effects of Xylooligosaccharide on Growth Performance, Serum Biochemical and Immune Indices and Intestinal Microflora Composition of Juvenile Japanese Seabass (Lateolabrax japonicus)[J]. Chinese Journal of Animal Nutrition, 2018 , 30(2) : 734 -742 . DOI: 10.3969/j.issn.1006-267x.2018.02.039

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