Effects of Stocking Density on Cecal Microflora Diversity, Cecal Volatile Fatty Acids and Serum Brain Gut Peptides of Broilers

CHANG Shuangshuang, LIU Qingxiu, ZHANG Minhong, SHI Yuxiang, FENG Jinghai

doi:10.3969/j.issn.1006-267x.2018.03.017

2018 , Vol. 30 >Issue 3: 938 - 946

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.03.017

Molecular Nutrition

Effects of Stocking Density on Cecal Microflora Diversity, Cecal Volatile Fatty Acids and Serum Brain Gut Peptides of Broilers

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Agriculture, Hebei University of Engineering, Handan 056021, China

Received date: 2017-08-15

Online published: 2018-03-05

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Abstract

The study was conducted to investigate the effects of stocking density on cecal microflora diversity, cecal volatile fatty acids and serum brain gut peptides of broilers. A single factor design was adopted in the experiment and one hundred and forty-four 22-day-old healthy Arbor Acres broilers with similar body weight were randomly divided into three groups, each group contained six cages and each cage as a replicate. The pre-test period lasted for 7 days and broilers were kept at 21℃ temperature and 60% relative humidity. When broilers were 29-day-old, with the area of single chicken cage was 0.64 m², the stocking density was 6 birds each cage (half male and half female, group Ⅰ), 8 birds each cage (half male and half female, group Ⅱ) and 10 birds each cage (half male and half female, group Ⅲ) respectively under 21℃ temperature and 60% relative humidity, and the trial lasted for 14 days. The results showed as follows:1) the richness and diversity of cecal microflora of broilers were the highest in group Ⅲ, and the stocking density in this group was beneficial to the growth of cecal Clostridium termitidis and Bacteroides vulgatus of broilers. 2) The influence of stocking density on the volatile fatty acid contents in the cecum of broilers was not significantly different (P>0.05). 3) At the 14^th day of the experiment, the serum vasoactive intestinal peptide (VIP) content of broilers in group Ⅲ was significantly higher than those in groups Ⅰ and Ⅱ (P<0.05), but there were no significant differences on serum contents of 5-hydroxytryptamine and substance P of broilers among three groups (P>0.05). In conclusion, the stocking density can change the diversity and structure of cecal microflora as well as serum VIP content, but does not affect the contents of volatile fatty acids in the cecum of broilers.

Key words： stocking density; cecal microflora; volatile fatty acid; brain gut peptide; broilers

Cite this article

CHANG Shuangshuang, LIU Qingxiu, ZHANG Minhong, SHI Yuxiang, FENG Jinghai . Effects of Stocking Density on Cecal Microflora Diversity, Cecal Volatile Fatty Acids and Serum Brain Gut Peptides of Broilers[J]. Chinese Journal of Animal Nutrition, 2018 , 30(3) : 938 -946 . DOI: 10.3969/j.issn.1006-267x.2018.03.017

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