Molecular Nutrition

Effects of Replacing Alfalfa Hay with Dry Corn Fiber Feed and Chinese leymus on in Vitro Rumen Fermentation

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  • 1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
    2. College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China

Received date: 2017-08-29

  Online published: 2018-03-05

Abstract

The aim of this study was to investigate the effects of replacing alfalfa hay with dry corn fiber feed (DCFF) and Chinese leymus on in vitro rumen fermentation of dairy cows. A diet for dairy cows was used as a fermentation substrate. Different proportions (0, 5.00%, 10.50%, 17.50% and 22.90%) of alfalfa hay in the substrate were replaced with combinations of DCFF and Chinese leymus (DCFF proportion in the fermentation substrate was 0, 3.00%, 7.00%, 11.00% and 15.00%, respectively), which were named 0DCFF, 3DCFF, 7DCFF, 11DCFF, 15DCFF groups, respectively. Fermentation parameters and microflora after fermented for 24 h, and gas production parameters after fermented for 48 h were determined. The results showed as follows:1) replacing alfalfa hay with DCFF and Chinese leymus had significant effects on gas production (GP), potential gas production and gas production rate of in vitro fermentation (P<0.05), which showed firstly increased and then decreased tendency with the increase of replacing proportion, and 11DCFF group had the highest values; replacing alfalfa hay with DCFF and Chinese leymus had significant effects on dry matter disappearance rate of in vitro fermentation (P<0.05), 11DCFF and 15DCFF groups were significantly higher than the other groups (P<0.05). 2) Replacing alfalfa hay with DCFF and Chinese leymus had significant effects on the concentrations of microbial protein (MCP), ammonia nitrogen, acetic acid, propionic acid, butyric acid and total volatile fatty acids (TVFA) in fermentation fluid (P<0.05), which showed firstly increased and then gentle tendency; replacing alfalfa hay with DCFF and Chinese leymus had significant effects on pH in fermentation fluid (P<0.05), which showed firstly decreased and then gentle tendency. 3) Replacing alfalfa hay with DCFF and Chinese leymus had significant effects on the relative counts of R. flavefaciens, F. succinogenes and B. fibrisolvens in fermentation fluid (P<0.05), which showed firstly increased and then gentle tendency; there were no significant difference of the relative counts of S. bovis, S. amylolytica, R. amylophilus, R. albus in fermentation fluid among groups (P>0.05). It is concluded that replacing alfalfa hay in diet for dairy cows with DCFF and Chinese leymus can improve in vitro rumen fermentation, when the replacing proportions are 17% and 23%, the effects are better, and the proportions of DCFF are 11.00% and 15.00% at this time, respectively.

Cite this article

HAO Xiaoyan, ZHANG Guangning, YAO Enyue, LIU Yan, SUN Kaijing, ZHANG Yonggen . Effects of Replacing Alfalfa Hay with Dry Corn Fiber Feed and Chinese leymus on in Vitro Rumen Fermentation[J]. Chinese Journal of Animal Nutrition, 2018 , 30(3) : 953 -962 . DOI: 10.3969/j.issn.1006-267x.2018.03.019

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