Effects of Glucose on Fertilized Eggs Hatch, Activities of Acetyl-CoA Carboxylase and Fatty Acid Synthase of Yolk-Sac Larvae of Hybrid Grouper (Epinephelus fuscoguttatus ♀&times;Epinephelus polyphekadion ♂)

HUANG Jiansheng, RUAN Tao, CHEN Gang, ZHANG Jiandong, WANG Zhongliang, TANG Baogui

doi:10.3969/j.issn.1006-267x.2018.03.022

2018 , Vol. 30 >Issue 3: 981 - 988

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.03.022

Molecular Nutrition

Effects of Glucose on Fertilized Eggs Hatch, Activities of Acetyl-CoA Carboxylase and Fatty Acid Synthase of Yolk-Sac Larvae of Hybrid Grouper (Epinephelus fuscoguttatus ♀×Epinephelus polyphekadion ♂)

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Fishery College, Guangdong Ocean University, Zhanjiang 524088, China

Received date: 2017-08-28

Online published: 2018-03-05

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Abstract

In order to investigate the effects of glucose on fertilized eggs hatch, and activities of acetyl-CoA carboxylase (ACC) and fatty acid synthase (FAS) of yolk-sac larvae of hybrid grouper, different concentrations of glucose were added to the water for hatching of hybrid grouper (Epinephelus fuscoguttatus ♀×Epinephelus polyphekadion ♂), which were 0, 2, 4, 6, 8 and 10 mg/L, respectively. The hatching rate and abnormality rate were observed, and the total length, activities of ACC and FAS of yolk-sac larvae at 1, 2 and 3 days of age were tested. The results showed as follows:hatching rate of fertilized eggs and abnormalities of newly hatched larvae were significantly affected by glucose concentration (P<0.05); the correlation between the concentration of glucose and hatching rate of fertilized eggs and abnormality rate of newly hatched larvae both could be described by quadratic equations. The optimum glucose concentration was 3.8 mg/L to reach the highest hatching rate of fertilized eggs. When the concentration of glucose was below 6 mg/L, hatching rate of fertilized eggs increased, while abnormality rate of newly hatched larvae decreased, total length of yolk-sac larvae increased with the increase of concentration of glucose. For yolk-sac larvae at 1, 2 and 3 days of age, activities of ACC and FAS in glucose supplemented group were significantly higher than those in control group (P<0.05). With the increase of concentration of glucose, the activities of ACC and FAS of larvae at 1 day of age increased; at 2 and 3 days of age, ACC activity of larvae displayed a ‘up-down’ trend and FAS activity showed a ‘rising-gentle’ trend. It is concluded that in artificial breeding of hybrid grouper, the supplementation of 6 mg/L glucose in hatching water can promote fertilized egg hatch and yolk-sac larvae development, and significantly increase ACC and FAS activities of yolk-sac larvae.

Key words： glucose; hybrid grouper; hatching rate; yolk-sac larvae; acetyl-CoA carboxylase; fatty acid synthase

Cite this article

HUANG Jiansheng, RUAN Tao, CHEN Gang, ZHANG Jiandong, WANG Zhongliang, TANG Baogui . Effects of Glucose on Fertilized Eggs Hatch, Activities of Acetyl-CoA Carboxylase and Fatty Acid Synthase of Yolk-Sac Larvae of Hybrid Grouper (Epinephelus fuscoguttatus ♀×Epinephelus polyphekadion ♂)[J]. Chinese Journal of Animal Nutrition, 2018 , 30(3) : 981 -988 . DOI: 10.3969/j.issn.1006-267x.2018.03.022

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