Molecular Nutrition

Effects of Deoxynivalenol on Apoptosis of IPEC-J2 Cells

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  • 1. School of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410208, China;
    2. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received date: 2017-09-01

  Online published: 2018-03-05

Abstract

This experiment was conducted to determine the effect of deoxynivalenol (DON) on the apoptosis of IPEC-J2 cells after different concentrations and time treatment. An appropriate exposure time (6, 12, and 24 hour) used CCK-8 assay to test for cell proliferation. The effects of DON on cell mitosis cycle and early cell apoptosis were examined by treated the IPEC-J2 cells with low concentration (200 ng/mL) and high concentrations (2 000 ng/mL) DON for 6, 12, 24 hours. The results showed as follows:1) DON significantly inhibited the growth of IPEC-J2 cells. At 200 and 2 000 ng/mL DON concentrations, the cell survival rate of 48 and 72 h was significantly lower than that of 24 h (P<0.05), and the cell survival rate of 72 h was significantly lower than that of 48 at 2 000 ng/mL DON concentration (P<0.05). 2) Different DON concentrations had different effects on cell cycle. The low concentration of DON mainly acted on mitosis stage S and interferes with the replication of DNA, but the high concentration of DON mainly acted on the mitotic phase G2/M and interferes with the synthesis of enzymes and spindle filament proteins. 3) DON for 6 hours, the early apoptosis rate and total apoptosis rate of low concentration DON group and high concentration DON group were significantly higher than those of control group (P<0.05); DON for 12 hours, the early apoptosis rate and total apoptosis rate of high concentration DON group were significantly higher than those of control group and low concentration DON group (P<0.05). In conclusion, DON can inhibit IPEC-J2 cells proliferation, low concentration of DON can caused cell mitosis to stay in the S phase, high concentration of DON can caused cell mitosis to stay in the G2/M period. DON also can promote cell apoptosis and increase total apoptosis rate, and this effect is enhanced with DON concentration increasing.

Cite this article

LIAO Meifang, MENG Yinchai, ZHAN Jihua, YIN Yulong, LIAO Peng, LI Ling . Effects of Deoxynivalenol on Apoptosis of IPEC-J2 Cells[J]. Chinese Journal of Animal Nutrition, 2018 , 30(3) : 1027 -1034 . DOI: 10.3969/j.issn.1006-267x.2018.03.027

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