Molecular Nutrition

Effects of Allium mongolicum Regel Flavonoids on Performance and β-Defensins Gene Expression in Intestinal Tissue of Meat Sheep

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  • College of animal science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2017-09-12

  Online published: 2018-03-05

Abstract

The objective of this study was to investigate the effects of Allium mongolicum Regel flavonoids on performance and the expression of immune factors-β-defensin 1 (sBD-1) and β-defensin 2 (sBD-2) in intestinal tissue of meat sheep. Single factor completely randomized block design was used in the current study, a total of 60 healthy and six-month-old thin-tailed Han wethers with similar body weight[(39.9±3.2) kg] were randomly divided into 4 groups with 15 sheep each. Sheep in control group were fed a basal diet, and those in experimental groups were fed the basal diet supplemented with 11 (low-dose flavonoids group), 22 (mid-dose flavonoids group) and 33 mg/kg Allium mongolicum Regel flavonoids (high-dose flavonoids group), respectively. The trial period lasted for 75 d, which consisted of 15 d of pre-feeding period and 60 d of formal period. Intake was recorded every day and sheep were weighted before feeding every 15 d. At the end of the experiment, three sheep were randomly selected from each group and slaughtered to collect the duodenum, jejunum and ileum tissues. The relative expression levels of genes involved in sBD-1 and sBD-2 were measured by real time-qPCR. The results showed as follows:1) at the 30th to 45th day of experiment, the average daily feed intake of meat sheep in experimental groups was significantly higher than that in control group (P<0.05), and the highest average daily feed intake was found in high-dose flavonoids group. At the 45th to 60th day of experiment, the average daily gain was significantly increased and the feed to gain ratio was significantly decreased in experimental groups compared with the control group (P<0.05), and the highest average daily gain and the lowest feed to gain ratio were found in mid-dose flavonoids group and high-dose flavonoids group, respectively. 2) Compared with the control group, diet supplemented with 33 mg/kg Allium mongolicum Regel flavonoids significantly increased the relative expression levels of sBD-1 gene in jejunum and ileum and sBD-2 gene in jejunum (P<0.05), and diet supplemented with 22 mg/kg Allium mongolicum Regel flavonoids significantly increased the relative expression levels of sBD-2 gene in duodenum (P<0.05). In conclusion, diet supplemented with 33 mg/kg Allium mongolicum Regel flavonoids can significantly increase the performance and the expression of β-defensins (sBD-1 and sBD-2) genes in intestine of meat sheep.

Cite this article

CHEN Shengyang, AO Changjin, ZHENG Yankai, CHEN Renwei, LIU Wangjing, MU Qier, BAI Chen . Effects of Allium mongolicum Regel Flavonoids on Performance and β-Defensins Gene Expression in Intestinal Tissue of Meat Sheep[J]. Chinese Journal of Animal Nutrition, 2018 , 30(3) : 1095 -1102 . DOI: 10.3969/j.issn.1006-267x.2018.03.034

References

[1] 赵春艳,敖长金,张兴夫.沙葱中黄酮类化合物对小鼠非特异性免疫机能影响的研究[J].饲料工业,2010,31(18):11-14.

[2] 缪亚娟.沙葱异黄酮含量测定及其对机体抗氧化与非特异性免疫影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2009:38-51.

[3] 萨茹丽.沙葱黄酮提取工艺优化、结构鉴定及其相关生物活性研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2014:18-26.

[4] ZHANG G L,ROSS C R,BLECHA F.Porcine antimicrobial peptides:new prospects for ancient molecules of host defense[J].Veterinary Research,2000,31(3):277-296.  

[5] TAYLOR K,BARRAN P,DORIN J.Review:structure-activity relationships in beta-defensin peptides[J].Biopolymers,2008,90(1):1-7.  

[6] GANZ T. Defensins:antimicrobial peptides of innate immunity.Nat.Rev.Immunol.3,710-720[J].Nature Reviews Immunology,2003,3(9):710-710.  

[7] RÖHRL J,GEISSLER E K,HEHLGANS T.Friend or foe:a novel role of β-defensins in tumor development[J].Oncoimmunology,2012,1(7):1159-1160.  

[8] 马卫明,佘锐萍,彭芳珍,等.猪小肠抗菌肽的提取及部分生物学活性研究[J].科学技术与工程,2004,4(3):202-205.

[9] 木其尔,敖长金,萨茹丽,等.沙葱总黄酮对肉羊抗氧化能力的影响[J].动物营养学报,2016,28(6):1823-1831.

[10] 陈仁伟.沙葱黄酮对肉羊生产性能及其肉品质的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2016:19-22.

[11] 木其尔.沙葱黄酮对肉羊机体抗氧化和免疫功能的影响及其机理研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2016:32-38.

[12] 萨茹丽,木其尔,王翠芳,等.沙葱黄酮对绵羊外周血淋巴细胞增殖及IL-2、IL-4、IFN-γ mRNA表达的影响[J].畜牧兽医学报,2015,46(6):1063-1070.

[13] 萨茹丽,木其尔,王翠芳,等.沙葱总黄酮提取工艺优化及其体外抗氧化、抗菌作用[J].食品科学,2014,35(24):1-8.

[14] WELLS J M,LOONEN L M,KARCZEWSKI J M.The role of innate signaling in the homeostasis of tolerance and immunity in the intestine[J].International Journal of Medical Microbiology,2010,300(1):41-48.  

[15] POMORSKAMÍI M,MARKOWSKADANIEL I.Porcine cathelicidins and defensins[J].Medycyna Weterynaryjna,2011,67(1):20-24.

[16] GALLO R L,KIM K J,BERNFIELD M,et al.Identification of CRAMP,a cathelin-related antimicrobial peptide expressed in the embryonic and adult mouse[J].Journal of Biological Chemistry,1997,272(20):13088-13093.  

[17] 唐浩国,魏晓霞,李叶,等.竹叶黄酮对小鼠脾细胞免疫的分子机制研究[J].食品科学,2007,28(9):523-526.

[18] 尹香花,成艳,崔素芬.大豆苷原和染料木黄酮对小鼠子宫内膜癌TNF-α、IL-1α及IL-6表达的影响[J].中国现代医学杂志,2010,20(6):832-835.

[19] GERSHWIN M E,GERMAN J B,KEEN C L.Nutrition and immunology:principles andpractice[M].New York:Humana Press,2000.

[20] BABU U S,RAYBOURNE R B.Impact of dietary components on chicken immune system and Salmonella infection.[J].Expert Review of Anti-Infective Therapy,2008,6(1):121-35.  

[21] 杨华,张韩杰,吴信明,等.微生态制剂对肉羊生长性能和免疫功能的影响[J].家畜生态学报,2015,36(10):27-32.
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