Effects of Chlorogenic Acid on Growth Performance, Serum Immunoglobulins, Intestinal Mucosa Morphology, Digestive and Absorptive Capacity of Piglets

WANG Yu, ZHOU Xuanwu, CHEN Daiwen, YU Bing, MAO Xiangbing, ZHENG Ping, YU Jie, LUO Yuheng, HUANG Zhiqing, LUO Junqiu, HE Jun

doi:10.3969/j.issn.1006-267x.2018.03.039

2018 , Vol. 30 >Issue 3: 1136 - 1145

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.03.039

Molecular Nutrition

Effects of Chlorogenic Acid on Growth Performance, Serum Immunoglobulins, Intestinal Mucosa Morphology, Digestive and Absorptive Capacity of Piglets

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Institute of Animal Nutrition, Sichuan Agricultural University, Chendu 611130, China

Received date: 2017-09-11

Online published: 2018-03-05

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Abstract

This study was aimed to investigate the effects of chlorogenic acid (CA) on growth performance, serum immunoglobulins, intestinal mucosa morphology, digestive and absorptive capacity of piglets. Twenty-four healthy Duroc×Landrace×Yorkshire weaned piglets with similar body weight of (9.45±0.20) kg were randomly allocated to 4 groups:1) control group, be given a basal diet; 2) CA250 group, be given the basal diet with 250 mg/kg CA; 3) CA500 group, be given the basal diet with 500 mg/kg CA; 4) CA1000 group, be given the basal diet with 1 000 mg/kg CA. Each group was represented by 6 replicates with 1 piglet per replicate. The experiment lasted for 14 days. The results showed as follows:1) there were no significant differences in average daily gain and average daily feed intake among all groups (P>0.05). The feed to gain ratio (F/G) in CA1000 group was significantly lower than that in control group (P<0.05). 2) No significant differences in serum contents of immunoglobulin A and immunoglobulin M were found among all groups (P>0.05). The serum immunoglobulin G content in CA1000 group was significantly higher than that in control group and CA250 group (P<0.05). 3) The duodenal villus height and villus to crypt ratio (V/C) in CA1000 group were significantly higher than those in control group (P<0.05). The duodenal crypt depth in experimental groups was significantly lower than that in control group (P<0.05). The villus height and width of jejunum in CA1000 group were significantly higher than those in control group (P<0.05). There were no significant differences in villus height, villus width, crypt depth and V/C of ileum among all groups (P>0.05). 4) The duodenal sucrase activity in experimental groups was increased, but showed no significant differences compared with control group (P>0.05). The duodenal maltase activity in experimental groups was significantly higher than that in control group (P<0.05). There were no significant differences in jejunal sucrase activity among all groups (P>0.05). The jejunal maltase activity in experimental groups was increased, but showed no significant differences compared with control group (P>0.05). There were no significant differences in ileal sucrase activity among all groups (P>0.05). The ileal maltase activity in CA1000 group was significantly higher than that in control group (P<0.05). 5) There were no significant differences in the relative expression of sodium-dependent glucose transporters 1 (SGLT1) and glucose transporter 2 (GLUT2) genes in duodenum among all groups (P>0.05). No significant differences in SGLT1 relative expression in jejunum were found among all groups (P>0.05). The GLUT2 relative expression in jejunum in CA1000 group was significantly higher than that in control group (P<0.05). The SGLT1 relative expression in ileum in experimental groups was increased compared with control group, and the SGLT1 relative expression in ileum in CA1000 group was significantly higher than that in control group (P<0.05). There were no significant differences in the GLUT2 relative expression in duodenum among all groups (P>0.05). In conclusion, it is suggested that the amount of CA supplementation in piglet diet is 1 000 mg/kg. The suggested amount of CA can reduce the F/G significantly, enhance the immune function, improve the intestinal mucosa morphology, promote the digestive and absorptive capacity, which in turn raise the growth performance of weaned piglets.

Key words： chlorogenic acid; piglet; growth performance; serum immunoglobulin; intestinal morphology; digestive and absorptive capacity

Cite this article

WANG Yu, ZHOU Xuanwu, CHEN Daiwen, YU Bing, MAO Xiangbing, ZHENG Ping, YU Jie, LUO Yuheng, HUANG Zhiqing, LUO Junqiu, HE Jun . Effects of Chlorogenic Acid on Growth Performance, Serum Immunoglobulins, Intestinal Mucosa Morphology, Digestive and Absorptive Capacity of Piglets[J]. Chinese Journal of Animal Nutrition, 2018 , 30(3) : 1136 -1145 . DOI: 10.3969/j.issn.1006-267x.2018.03.039

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