Molecular Nutrition

Measurement of Nano Elemental Selenium in Selenium-Enriched Polysaccharides Using High Performance Liquid Chromatography Coupled With Inductivity Coupled Plasma Mass Spectrometry Method

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  • Feed Science Institute of Zhejiang University, National Engineering Laboratory of Bio-Feed Safety and Pollution Prevention, Key Laboratory of Animal Nutrition and Feed Science(Huadong), Ministry of Agriculture, Hangzhou 310058, China

Received date: 2017-08-31

  Online published: 2018-03-05

Abstract

This experiment was conducted to establish a novel method which could efficiently, accurately and quickly determine the nano elemental selenium (Nano-Se) content extracted from selenium-enriched polysaccharides. Approximately 3 mg of protease ⅩⅣ was added into 3 mL of H2O contained 25 mg selenium-enriched polysaccharides which produced by Enterobacter cloacae Z0206, and the resulting mixture was maintained at 37℃ in an ultrasonic bath for 30 min. After centrifugation, the precipitate (Nano-Se) was oxidized by HCl-H2O2 system at room temperature. Then the consequent solution was directly analyzed by high performance liquid chromatography coupled with inductivity coupled plasma mass spectrometry (HPLC-ICP-MS) (equipped with PRP-X100 anion column and the mobile phase was 5 mmol/L citric acid with a pH of 4.5) and the content of Nano-Se was 2 439 μg/g. As a contrast, the same samples were oxidized by HNO3-HClO4 system and determined by the method described in GB/T 13883-2008 using hydride generation atomic fluorescence spectrometry (HG-AFS) and the content of Nano-Se was 2 450 μg/g which was similar to the result above. Compare with the method described in GB/T 13883-2008, the oxidation system in the current method is much milder and faster and easy to control which is suitable for Nano-Se detection.

Cite this article

WANG Fengqin, CHENG Yuanzhi, XIAO Xiao, LI Xiaoxiao, SONG Deguang, LU Zeqing, WANG Yizhen . Measurement of Nano Elemental Selenium in Selenium-Enriched Polysaccharides Using High Performance Liquid Chromatography Coupled With Inductivity Coupled Plasma Mass Spectrometry Method[J]. Chinese Journal of Animal Nutrition, 2018 , 30(3) : 1155 -1161 . DOI: 10.3969/j.issn.1006-267x.2018.03.041

References

[1] KESSI J,HANSELMANN K W.Similarities between the abiotic reduction of selenite with glutathione and the dissimilatory reaction mediated by Rhodospirillum rubrum and Escherichia coli[J].Journal of Biological Chemistry,2004,279(49):50662-50669.  

[2] LOESCHNER K,HADRUP N,HANSEN M,et al.Absorption,distribution,metabolism and excretion of selenium following oral administration of elemental selenium nanoparticles or selenite in rats[J].Metallomics,2014,6(2):330-337.  

[3] VIAMAJALA S,BEREDED-SAMUEL Y,APEL W A,et al.Selenite reduction by a denitrifying culture:batch-and packed-bed reactor studies[J].Applied Microbiology and Biotechnology,2006,71(6):953-962.  

[4] NOGUEIRA C W,ROCHA J B T.Toxicology and pharmacology of selenium:emphasis on synthetic organoselenium compounds[J].Archives of Toxicology,2011,85(11):1313-1359.  

[5] WANG H L,ZHANG J S,YU H Q.Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes:comparison with selenomethionine in mice[J].Free Radical Biology and Medicine,2007,42(10):1524-1533.  

[6] ZHANG J S,WANG X F,XU T W.Elemental selenium at nano size (nano-Se) as a potential chemopreventive agent with reduced risk of selenium toxicity:comparison with Se-methylselenocysteine in mice[J].Toxicological Sciences,2008,101(1):22-31.  

[7] DEBIEUX C M,DRIDGE E J,MUELLER C M,et al.A bacterial process for selenium nanosphere assembly[J].Proceedings of the National Academy of Sciences of the United States of America,2011,108(33):13480-13485.  

[8] JAIN R,JORDAN N,WEISS S,et al.Extracellular polymeric substances govern the surface charge of biogenic elemental selenium nanoparticles[J].Environmental Science & Technology,2015,49(3):1713-1720.  

[9] ZHENG S X,SU J,WANG L,et al.Selenite reduction by the obligate aerobic bacterium Comamonas testosteroni S44 isolated from a metal-Contaminated soil[J].BMC Microbiology,2014,14(1):204-230.  

[10] LIAO W Z,YU Z Q,LIN Z H,et al.Biofunctionalization of selenium nanoparticle with Dictyophora indusiata polysaccharide and its antiproliferative activity through death-receptor and mitochondria-mediated apoptotic pathways[J].Scientific Reports,2015,5:18629.

[11] JIN M L,WANG Y M,XU C L,et al.Preparation and biological activities of an exopolysaccharide produced by Enterobacter cloacae Z0206[J].Carbohydrate Polymers,2010,81(3):607-611.  

[12] LU Z Q,JIN M L,HUANG M,et al.Bioactivity of selenium-enriched exopolysaccharides produced by Enterobacter cloacae Z0206 in broilers[J].Carbohydrate Polymers,2013,96(1):131-136.  

[13] XU C L,WANG Y Z,JIN M L,et al.Preparation,characterization and immunomodulatory activity of selenium-enriched exopolysaccharide produced by bacterium Enterobacter cloacae Z0206[J].Bioresource Technology,2009,100(6):2095-2097.  

[14] SOND D G,CHENG Y Z,LI X X,et al. Biogenic nano-selenium particles effectively attenuate oxidative stress-induced intestinal epithelial barrier injury by activating the Nrf2 antioxidant pathway[J].ACS Applied Materials & Interfaces,2017,9(17):14724-14740.  

[15] SULLIVAN D,ZYWICKI R,YANCEY M.Method for the determination of total selenium in a wide variety of foods using inductively coupled plasma/mass spectrometry[J].Journal of AOAC International,2013,96(4):786-794.  

[16] DUFAILLY V,NOËL L,GUÉRIN T.Determination of chromium,iron,and selenium in foodstuffs of animal origin by collision cell technology,inductively coupled plasma mass spectrometry (ICP-MS) after closed vessel microwave digestion[J].Analytica Chimica Acta,2006,565(2):214-221.  

[17] CREWS H M,DUCROS V,EAGLES J,et al.Mass spectrometric methods for studying nutrient mineral and trace element absorption and metabolism in humans using stable isotopes.A review[J].Analyst,1994,119(11):2491-2514.  

[18] DIAL A R,MISRA S,LANDING W M.Determination of low concentrations of iron,arsenic,selenium,cadmium,and other trace elements in natural samples using an octopole collision/reaction cell equipped quadrupole-inductively coupled plasma mass spectrometer[J].Rapid Communications in Mass Spectrometry,2015,29(8):707-718.  

[19] EL ATI-HELLAL M,HELLAL F,HEDHILI A.Application of plackett-burman and doehlert designs for optimization of selenium analysis in plasma with electrothermal atomic absorption spectrometry[J].Clinical Biochemistry,2014,47(15):95-100.  

[20] THOSAIKHAM W,JITMANEE K,SITTIPOUT R,et al.Evaluation of selenium species in selenium-enriched pakchoi (Brassica chinensis Jusl var parachinensis (Bailey) Tsen & Lee) using mixed ion-pair reversed phase HPLC-ICP-MS[J].Food Chemistry,2014,145:736-742.

[21] WELNA M,SZYMCZYCHA-MADEJA A,POHL P.Improvement of determination of trace amounts of arsenic and selenium in slim coffee products by HG-ICP-OES[J].Food Analytical Methods,2014,7(5):1016-1023.  

[22] 倪润祥,雒昆利.湿消解-原子荧光法测定煤中硒和砷[J].光谱学与光谱分析,2015,35(5):1404-1408.

[23] 俞宏明.基于HPLC-ICP-MS技术分析测试常见食品及富硒食品中不同形态硒含量的研究[D].硕士学位论文.杭州.浙江工业大学,2015,22-25.

[24] 王欣,幸苑娜,陈泽勇,等.高效液相色谱-电感耦合等离子体质谱法检测富硒食品中6种硒形态[J].分析化学,2013,41(11):1669-1674.

[25] GODIN S,FONTAGNÉ-DICHARRY S,BUENO M,et al.Influence of dietary selenium species on selenoamino acid levels in rainbow trout[J].Journal of Agricultural and Food Chemistry,2015,63(28):6484-6492.  

[26] JAGTAP R,MAHER W,KRIKOWA F,et al.Measurement of selenomethionine and selenocysteine in fish tissues using HPLC-ICP-MS[J].Microchemical Journal,2016,128:248-257.

[27] KLOGNEROVÁ K,VOSMANSKÁ M,SZÁKOVÁ J,et al.Effect of growing conditions on selenium speciation in rapeseed (Brassica napus) tissue[J].Chemicke Listy,2015,109(3):216-222.

[28] KROFLI? A,GERM M,MECHORA Š,et al.Selenium and its compounds in aquatic plant Veronica anagallis-aquatica[J].Chemosphere,2016,151:296-302.

[29] STANCIU-BURILEANU M M,CONSTANTIN L,RADU L,et al.Determination of selenium content in soil and parent material by HG-AAS[J].Revista de Chimie (Bucharest,Romania) 2012,63(6):553-556.

[30] 连建洲,黄逸文,李重庆,等.铜铟镓硒的回收方法:中国,CN201010214393.8[P].2010-06-25.
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