A Study on in Vitro Rumen Fermentation Characteristics of Potato Vines and Leaves Mixed Silage Based on Rumen Simulation Technique

LUO Ruirui, GUO Yanli, HAN Haizhu, LI Taotao, SUI Jingwei, FENG Peigong

doi:10.3969/j.issn.1006-267x.2018.03.045

2018 , Vol. 30 >Issue 3: 1185 - 1191

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.03.045

Molecular Nutrition

A Study on in Vitro Rumen Fermentation Characteristics of Potato Vines and Leaves Mixed Silage Based on Rumen Simulation Technique

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College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2017-09-12

Online published: 2018-03-05

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Abstract

This study was conducted to investigate the in vitro rumen fermentation characteristics of potato vines and leaves mixed silage using rumen simulation technique (Rusitec). A complete one-factor randomized design was used. Eight fermentation tanks of Rusitec system were divided into two groups with 4 replicates per group and 1 tank per replicate. Corn stalk silage and potato vines and leaves mixed silage[potato vines and leaves and corn stalk (40:60) were mixed for silage] were used to prepare diets for sheep, respectively, and the two groups used the diets as fermentation substrates, respectively. The study consisted of an 8 d pre-test period and a 2 d sampling period. The results showed as follows:compared with corn stalk silage group, potato vines and leaves mixed silage group had significantly higher gas production, contents of total nitrogen, ammonia nitrogen (except 3 h after substrate replacement) and microbial nitrogen in fermentation fluid for whole period, and propionic acid content in fermentation fluid at 6 and 9 h after substrate replacement (P<0.05), and had significantly lower methane production, fermentation fluid pH at 3 h after substrate replacement, acetic acid content in fermentation fluid at 9 h after substrate replacement, and acetic acid/propionic acid in fermentation fluid at 6 and 9 h after substrate replacement (P<0.05). There were no significant effects on nutrients degradation rate and other rumen fermentation parameters (P>0.05). These results indicate that potato vines and leaves mixed silage has no negative effects on in vitro rumen fermentation, can reduce methane pollution, and promote the utilization of energy and nitrogen in ruminants.

Key words： potato vines and leaves; silage; rumen fermentation; rumen simulation technique

Cite this article

LUO Ruirui, GUO Yanli, HAN Haizhu, LI Taotao, SUI Jingwei, FENG Peigong . A Study on in Vitro Rumen Fermentation Characteristics of Potato Vines and Leaves Mixed Silage Based on Rumen Simulation Technique[J]. Chinese Journal of Animal Nutrition, 2018 , 30(3) : 1185 -1191 . DOI: 10.3969/j.issn.1006-267x.2018.03.045

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