Protective Effects of Betulinic Acid on Immune Organs of Oxidative Damage Mice Induced by Cyclophosphamide

YI Xianglian, ZHU Lijuan, ZHAO Jing, WEN Lixin, POZNIAK Blazej, LUO Chenxi, HUANG Shuxian, WU Jing, YI Jin'e

doi:10.3969/j.issn.1006-267x.2018.03.047

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 3: 1199 - 1206

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.03.047

Molecular Nutrition

Protective Effects of Betulinic Acid on Immune Organs of Oxidative Damage Mice Induced by Cyclophosphamide

Expand

1. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;
2. Changsha Lvye Bio-Technology Co., Ltd., Changsha 410125, China;
3. Hunan Co-Innovation Center of Livestock Production Safety, Changsha 410128, China;
4. Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland

Received date: 2017-09-12

Online published: 2018-03-05

Fold

Abstract

The objective of this research was to evaluate the effects of betulinic acid (BA) on immune organs of oxidative damaged mice induced by cyclophosphamide(Cy). Fifty male healthy Kunming mice were randomly divided into 5 groups, which were control group, Cy group, and 0.05, 0.50 and 5.00 mg/kg BW BA groups. Control and Cy groups were orally administered with 1% starch solution, and the other groups were orally administered with different doses of BA for 14 days. Except control group, mice in the other groups were intraperitoneal injected Cy at the dosage of 50 mg/kg BW to set up oxidative damage model. Serum, spleen and thymus of mice were collected. Activities of alanine aminotransferase (ALT) and aspartate transaminase (AST), contents of total protein (TP) and albumin (ALB) in serum, as well as activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalast (CAT), and contents of glutathione (GSH) and malondialdehyde (MDA) in spleen and thymus were determined. The results showed as follows:1) about body weight, control group and Cy group had no significant difference (P>0.05), and BA groups had no significant difference with Cy group (P>0.05). 2) Compared with control group, Cy significantly increased serum AST and ALT activities (P<0.01), and 0.05 mg/kg BW BA significantly relieved the effects of Cy (P<0.01). 3)Compared with control group, Cy significantly decreased thymus index (P<0.05), and 0.05 mg/kg BW BA significantly relieved the effects of Cy (P<0.05). 4)Compared with control group, Cy significantly decreased SOD and CAT activities in thymus, and GSH content in spleen (P<0.05 or P<0.01), significantly increased spleen MDA content (P<0.01); compared with Cy group, 0.05 mg/kg BW BA group had significantly higher GSH-Px and CAT activities in spleen and thymus (P<0.05 or P<0.01), and significantly lower MDA content in spleen (P<0.01), but had significantly lower SOD activity in thymus (P<0.01); compared with Cy group, 5.00 mg/kg BW BA group had significantly higher GSH-Px and CAT activities in spleen and thymus(P<0.05 or P<0.01), and significantly lower MDA content in spleen (P<0.01). The results suggest that BA can effectively improve oxidative stress of immune organs and shows preventive protection of oxidative damage in mice induced by Cy.

Key words： betulinic acid; cyclophosphamide; immune organ; oxidative stress

Cite this article

YI Xianglian, ZHU Lijuan, ZHAO Jing, WEN Lixin, POZNIAK Blazej, LUO Chenxi, HUANG Shuxian, WU Jing, YI Jin'e . Protective Effects of Betulinic Acid on Immune Organs of Oxidative Damage Mice Induced by Cyclophosphamide[J]. Chinese Journal of Animal Nutrition, 2018 , 30(3) : 1199 -1206 . DOI: 10.3969/j.issn.1006-267x.2018.03.047

References

[1] FUJIOKA T,KASHIWADA Y,KILKUSKIE R E,et al.Anti-AIDS agents,11.Betulinic acid and platonic acid as anti-HIV principles from Syzigium claviflorum,and the anti-HIV activity of structurally related triterpenoids[J].Journal of Natural Products,1994,57(2):243-247.

[2] 朱若岑,夏伟,谭柱良,等.桦木酸对Dex致小鼠氧化损伤的保护作用[J].中国兽医学报,2016,36(2):305-309.

[3] 邱静娟.俄罗斯人与白桦树[J].俄语学习,2000(2):45-47.

[4] 易金娥,邬静,文利新,等.桦木酸的药理作用研究进展[J].中草药,2014,45(14):2118-2124.

[5] PISAH E,CHAI H,LEE I S,et al.Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis[J].Nature Medicine,1995,1(10):1046-1051.

[6] CICHEWICZ R H,KOUZI S A.Chemistry,biological activity,and chemotherapeutic potential of betulinic acid for the prevention and treatment of cancer and HIV infection[J].Medicinal Research Reviews,2004,24(1):90-114.

[7] 韦凤华.环磷酰胺临床应用研究进展[J].中国药事,2013,27(3):324-326.

[8] 王光,潘江球,钟杰平,等.乌贼墨提取物对环磷酰胺致小鼠脾脏氧化性损伤的保护效应[J].食品科学,2009,30(11):219-222.

[9] 张秀娟,凌莉莉,季宇彬.桦木酸生物活性研究进展[J].天然产物研究与开发,2006,18(3):508-513.

[10] 吴皓,林洪生,裴迎霞,等.人参皂甙Rg3对荷瘤及环磷酰胺化疗小鼠黏膜免疫力影响[J].中国肿瘤,2006,15(6):369-371.

[11] ALAKURTTI S,MÄKELÄ T,KOSKIMIE S,et al.Pharmacological properties of the ubiquitous natural product betulin[J].European Journal of Pharmaceutical Sciences,2006,29(1):1-13.

[12] TRIPATHI D N,JENA G B.Intervention of astaxanthin against cyclophosphamide-induced oxidative stress and DNA damage:a study in mice[J].Chemico-Biological Interactions,2009,180(3):398-406.

[13] OBOH G,AKOMOLAFE T L,ADEFEGHA S A,et al.Inhibition of cyclophosphamide-induced oxidative stress in rat brain by polar and non-polar extracts of Annatto (Bixa orellana) seeds[J].Experimental and Toxicologic Pathology,2011,63(3):257-262.

[14] RAVID A,ROCKER D,MACHLENKIN A,et al.1,25-dihydroxyvitamin D3 enhances the susceptibility of breast cancer cells to doxorubicin-induced oxidative damage[J].Cancer Research,1999,59(4):862-867.

[15] 赵弋清,罗霞,陈东辉,等.不同剂量环磷酰胺诱导正常小鼠免疫抑制的对比研究[J].免疫学杂志,2005,21(3):122-124.

[16] 周华,王培训,刘良,等.环磷酰胺对小鼠Peyer's结和肠道黏膜相关淋巴细胞的影响[J].中国免疫学杂志,2000,17(4):186-187,189.

[17] 董晓芳,汪仕奎,萨仁娜,等.免疫抑制剂环磷酰胺对肉仔鸡生产性能及其内分泌的影响[J].畜牧兽医学报,2007,38(9):993-998.

[18] FULDA S,DEBATIN K M.Sensitization for anticancer drug induced apoptosis by betulinic acid[J].Neoplasia,2005,7(2):162-170.

[19] YOON J J,LEE Y J,KIM J S,et al.Protective role of betulinic acid on TNF-alpha-induced cell adhesion molecules in vascular endothelial cells[J].Biochemical and Biophysical Research Communications,2010,391(1):96-101.

[20] YI J N,XIA W,WU J P,et al.Betulinic acid prevents alcohol-induced liver damage by improving the antioxidant system in mice[J].Journal of Veterinary Science,2014,15(1):141-148.

[21] 袁莉芸,袁慧,陈铁桥.低剂量T-2毒素对雏鸡免疫器官及血清生化指标的影响[J].中国家禽,2007,29(17):14-16.

[22] 姜昕,刘密凤,林林,等.低剂量T-2毒素对大鼠血清生化指标的影响[J].中国地力病学杂志,2004,23(2):116-118.

[23] TSAI J C,PENG W H,CHIU T H,et al.Anti-inflammatory effects of Scoparia dulcis L. and betulinic acid[J].The American Journal of Chinese Medicine,2011,39(5):943-956.

[24] YI J,ZHU R C,WU J P,et al.In vivo protective effect of betulinic acid on dexamethasone induced thymocyte apoptosis by reducing oxidative stress[J].Pharmacological Reports,2016,68(1):95-100.

[25] YI J E,OBMINSKA-MRUKOWICZ B,YUAN L Y,et al.Immunomodulatory effects of betulinic acid from the bark of white birch on mice[J].Journal of Veterinary Science,2010,11(4):305-313.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References