Molecular Nutrition

Effects of Dietary Neutral Detergent Fibre Level on Structure and Composition of Rumen Bacteria in Goats

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  • Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2017-10-11

  Online published: 2018-04-03

Abstract

This study was conducted to investigate the effects of dietary neutral detergent fibre (NDF) level on structure and composition of rumen bacteria in goats using high throughput sequencing technique. Six goats were used in a 3×3 Latin square design and divided into 3 groups according to dietary NDF level, which was low (LN group, 35.01%), medium (MN group, 40.10%) and high NDF level groups (HN group, 45.16%), and each group had 2 goats. The feeding trial lasted for 3 stages with 20 d per stage, and each stage consisted of 14 d of pre-trial period and 6 d of trial period. After the trial period, rumen contents were collected to extract bacterial total DNA. The bacteria universal primers were used to amplify the V4 region of bacteria 16S rRNA by PCR. The sequencing of the amplified products of bacteria was performed using the Illumina HiSeq 250PE sequencing platform, and the results were analyzed by bioinformatics software such as QⅡME 1.8.0. The results showed as follows:1) rumen fluid ammonia nitrogen (NH3-N) concentration of HN group was significantly lower than that of LN and MN groups (P<0.01). Rumen fluid acetate/propionate of LN group was significantly lower than that of HN group (P<0.05), but there was no significant difference between MN group and other groups (P>0.05). 2) The differences of Chao1 and Shannon indexes among groups were not significant (P>0.05); observed species index of LN group was significantly higher than that of the other two groups (P<0.05), but there was no significant difference between MN group and HN group (P>0.05). 3) At phylum level, the relative abundance of all bacteria of three groups was not significant (P>0.05); at genus level, the relative abundances of Prevotellaceae UCG-001, Prevotellaceae UCG-003 and Ruminococcaceae UCG-014 of HN group were significant higher than those of the other two groups (P<0.05); the relative abundances of Ruminococcaceae NK4A214 group and Ruminococcaceae UCG-005 of HN group were significant higher than those of LN groups (P<0.05); the relative abundances of SP3-e08 and Lachnoclostridium 10 of HN group were significantly lower than those the other two groups (P<0.05); the relative abundance of Succiniclasticum of LN group was significantly higher than that of the other two groups (P<0.05); the relative abundances of Lysinibacillus, Bacillus and Phyllobacterium of MN group were significantly higher than those of the other two groups (P<0.05), and the relative abundance of Victivallis of MN group was extremely significantly higher than that of the other two groups (P<0.01). These results suggest that when dietary NDF level changing at 35.01% to 45.16%, NDF level significantly affects NH3-N concentration and acetate/propionate in rumen fluid, and the relative abundance of various bacterial genera, such as Prevotellaceae UCG-001 and Prevotellaceae UCG-003 in rumen of goats.

Cite this article

ZHANG Xuejiao, WANG Lizhi . Effects of Dietary Neutral Detergent Fibre Level on Structure and Composition of Rumen Bacteria in Goats[J]. Chinese Journal of Animal Nutrition, 2018 , 30(4) : 1377 -1386 . DOI: 10.3969/j.issn.1006-267x.2018.04.020

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