Effects of Cyanotis arachnoidea C. B. Clarke Grinding Granularity and Supplemental Level on Growth Performance, Hepatopancreas Digestive Enzyme Activities and Nonspecific Immune Indices of Macrobrachium nipponensis

ZHANG Yixiang, DING Zhili, WU Chenglong, MING Jianhua, YANG Xia, SHAO Xianping, KONG Youqin, YE Jinyun

doi:10.3969/j.issn.1006-267x.2018.04.022

2018 , Vol. 30 >Issue 4: 1396 - 1405

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.04.022

Molecular Nutrition

Effects of Cyanotis arachnoidea C. B. Clarke Grinding Granularity and Supplemental Level on Growth Performance, Hepatopancreas Digestive Enzyme Activities and Nonspecific Immune Indices of Macrobrachium nipponensis

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National-Local Joint Engineering Laboratory of Aquatic Animal Genetic Breeding and Nutrition(Zhejiang), Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, School of Life Sciences, Huzhou University, Huzhou 313000, China

Received date: 2017-09-03

Online published: 2018-04-03

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Abstract

This experiment were conducted to evaluate the effects of Cyanotis arachnoidea C. B. Clarke (C. arachnoidea) which was rich in ecdysone on the growth performance, hepatopancreas digestive enzyme activities and nonspecific immune indices of Macrobrachium nipponensis. In trial 1, C. arachnoidea with different grinding granularities (grinding granularity was 10, 30, 50 and 180 μm, respectively) were added into a basal diet (without C. arachnoidea), and to make the ecdysone content in experimental diets was all 10 mg/kg; in trial 2, C. arachnoidea with the same grinding granularity (grinding granularity was 180 μm) at different ecdysone contents were added into the basal diet, and to make the ecdysone content in experimental diets was 3.30, 6.60, 13.20, 26.40 mg/kg, respectively. There were 8 experimental diets were prepared, which were used to fed Macrobrachium nipponensis with the average body weight of (0.08±0.01) g for 60 days. One control group was set in trials 1 and 2, respectively, and Macrobrachium nipponensis in control group were fed the basal diet. Each diet had 3 replicates with 70 shrimps in each replicate. After the feeding experiment, the prawns were challenged with Aeromonas hydrophila. The results showed as follows:in the trial 1, compared with the control group, the weight gain rate (WGR), specific growth rate (SGR) in the groups with different grinding granularities of C. arachnoidea were significantly increased (P<0.05), and the feed conversion ratio (FCR) was significantly decreased (P<0.05); there were no significant differences in the survival rate (SR), hepatopancreas index (HSI), the activities of pepsin, tryptase and amylase in hepatopancreas, total hemocyte count (THC), haemolymph phagocytic (HPA), the activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in plasma among all groups (P>0.05); the cumulative mortality was not significantly different among all groups after treatment with Aeromonas hydrophila (P>0.05). In the trial 2, compared with the control group, the WGR and SGR in the 6.60 and 13.20 mg/kg ecdysone groups were significantly increased (P<0.05) and their FCR were significantly decreased (P<0.05); the WGR, SGR, SR and HPA in the 26.40 mg/kg ecdysone group were significantly lower than those in the control group (P<0.05), and the WGR and SGR in the 26.40 mg/kg ecdysone group were also significantly lower than those in the other C. arachnoidea additon groups (P<0.05), and there were no significant differences in WGR and SGR among 3.30, 6.60 and 13.20 mg/kg ecdysone groups (P>0.05); there were no significant differences in the HSI, the activities of pepsin, tryptase and amylase in hepatopancreas, THC, the activities of SOD and AKP in plasma among all groups (P>0.05); the cumulative mortality in 26.40 mg/kg ecdysone group was significantly higher than that of other groups after treatment with Aeromonas hydrophila (P<0.05). These results demonstrate that, the addition of the appropriate amount of C. arachnoidea to the diets making diet ecdysone contents are 6.60 to 13.20 mg/kg can significantly promote the growth of Macrobrachium nipponensis, and the absorption of ecdysone by Macrobrachium nipponensis has no significant correlation with the grinding granularity of C. arachnoidea. The main medicinal ingredient of C. arachnoidea, ecdysone has no immune enhancement effect on Macrobrachium nipponensis, and excess C. arachnoidea ecdysone (diet ecdysone content is 26.40 mg/kg) can lead SR and anti-bacterial ability.

Key words： Cyanotis arachnoidea C. B. Clarke; Macrobrachium nipponensis; growth; digestive enzyme; nonspecific immune

Cite this article

ZHANG Yixiang, DING Zhili, WU Chenglong, MING Jianhua, YANG Xia, SHAO Xianping, KONG Youqin, YE Jinyun . Effects of Cyanotis arachnoidea C. B. Clarke Grinding Granularity and Supplemental Level on Growth Performance, Hepatopancreas Digestive Enzyme Activities and Nonspecific Immune Indices of Macrobrachium nipponensis[J]. Chinese Journal of Animal Nutrition, 2018 , 30(4) : 1396 -1405 . DOI: 10.3969/j.issn.1006-267x.2018.04.022

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