Molecular Nutrition

Effects of Porcine Skeletal Muscle Satellite Cells on Lipid Deposition in Intramuscular Preadipocytes in Co-Culture System

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  • 1. College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China;
    2. Tianjin Institute of Animal Husbandry and Veterinary Medicine, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China;
    3 Institute of Chemical Technology, Tianjin University, Tianjin 300072, China

Received date: 2017-11-20

  Online published: 2018-04-03

Abstract

This experiment was conducted to study the effects of porcine skeletal muscle satellite cells on lipid deposition in intramuscular preadipocytes in co-culture system. Porcine skeletal muscle satellite cells and intramuscular preadipocytes were isolated and cultured, after the two cells were isolated and identified, they were co-cultured in Transwell cell co-culture plates, and the cells were inducted differentiation with the density above 90%. The intramuscular preadipocytes differentiation level, the expression of differentiation marker gene and the key enzymes in lipid metabolism were detected after differentiation of skeletal muscle satellite cells for 8 days. The results showed that compared with intramuscular preadipocytes alone culture, in the co-culture system, the lipid droplet number and lipid droplet area in intramuscular preadipocytes were significantly decreased (P<0.01), the gene and protein expressions of peroxisome proliferators-activated receptor γ and CCAAT enhancer binding protein α in intramuscular preadipocytes were significantly decreased (P<0.01), the gene and protein expressions of acetyl CoA carboxylase and fatty acid synthase in intramuscular preadipocytes were significantly decreased (P<0.01). It is concluded that porcine skeletal muscle satellite cells have inhibitory effect on lipid deposition in intramuscular preadipocytes in the co-culture system.

Cite this article

MA Yanxuan, WANG Wenjie, MU Shuqin, XI Jingning, LI Ning, ZHENG Zi, YAN Jun, SUN Chao . Effects of Porcine Skeletal Muscle Satellite Cells on Lipid Deposition in Intramuscular Preadipocytes in Co-Culture System[J]. Chinese Journal of Animal Nutrition, 2018 , 30(4) : 1423 -1430 . DOI: 10.3969/j.issn.1006-267x.2018.04.025

References

[1] KOKTA T,DODSON M V,GERTLER A,et al.Intercellular signaling between adipose tissue and muscle tissue[J].Domestic Animal Endocrinology,2004,27(4):303-331.  

[2] QUINN L S,STRAIT-BODEY L,ANDERSON B G,et al.Interleukin-15 stimulates adiponectin secretion by 3T3-L1 adipocytes:evidence for a skeletal muscle-to-fat signaling pathway[J].Cell Biology International,2005,29(6):449-457.  

[3] DODSON M V,HOSSNER K L,VIERCK J L.Intercellular communication between muscle cells and 3T3-L1 preadipocytes[J].Basic & Applied Myology Bam,1996,6(6):503-512.

[4] DODSON M V,VIERCK J L,HOSSNER K L,et al.The development and utility of a defined muscle and fat co-culture system[J].Tissue & Cell,1997,29(5):517-524.  

[5] DIETZE D,KOENEN M,RÖHRIG K,et al.Impairment of insulin signaling in human skeletal muscle cells by co-culture with human adipocytes[J].Diabetes,2002,51(8):2369-2376.  

[6] HAUSMAN G J,POULOS S P.A method to establish co-cultures of myotubes and preadipocytes from collagenase digested neonatal pig semitendinosus muscles[J].Journal of Animal Science,2005,83(5):1010-1016.  

[7] SASAO N,HIRAYAMA E,KIM J.Formation and characterization of spontaneously formed heterokaryons between quail myoblasts and 3T3-L1 preadipocytes:correlation between differential plasticity and degree of differentiation[J].European Journal of Cell Biology,2004,83(1):35-45.  

[8] CHOI S H,CHUNG K Y,JOHNSON B J,et al.Co-culture of bovine muscle satellite cells with preadipocytes increases PPARγ and C/EBPβ gene expression in differentiated myoblasts and increases GPR43 gene expression in adipocytes[J].Journal of Nutritional Biochemistry,2013,24(3):539-543.  

[9] 陈妍,张国华,陆会宁,等.八眉猪肌内前体脂肪细胞的分离培养及诱导分化[J].广东农业科学,2014,41(13):110-113,123.

[10] YANG J J,LIY H,WANG K F,et al.Isolation,culture and biological characteristics of multipotent porcine skeletal muscle satellite cells[J].Cell and Tissue Banking,2017,18(4):513-525.  

[11] YAN J,GAN L,YANG H L,et al.The proliferation and differentiation characteristics of co-cultured porcine preadipocytes and muscle satellite cells in vitro[J].Molecular Biology Reports,2013,40(4):3197-3202.  

[12] SUN C,QI R L,WANG L,et al.p38 MAPK regulates calcium signal-mediated lipid accumulation through changing VDR expression in primary preadipocytes of mice[J].Molecular Biology Reports,2012,39(3):3179-3184.  

[13] AILHAUD G,P GRIMALDI A,NÉGREL R.Cellular and molecular aspects of adipose tissue development[J].Annual Review of Nutrition,1992,12(1):207-233.  

[14] LI X Z,YAN C G,YU J,et al.Dietary whole and cracked linseed increases the proportion of oleic and α-linolenic acids in adipose tissues and decreases stearoyl-coenzyme A desaturase,acetyl-coenzyme A carboxylase,and fatty acid synthase gene expression in the longissimus thoracis muscle of Yanbian yellow cattle[J].Journal of Animal Science,2017,95(2):718-726.  

[15] FERRAND N,BÉREZIAT V,MOLDES M,et al.WISP1/CCN4 inhibits adipocyte differentiation through repression of PPARγ activity[J].Scientific Reports,2017,7:1749.

[16] CHOI S K,PARK S,JANG S B,et al.Cascade regulation of PPARγ and C/EBPα signaling pathways by celastrol impairs adipocyte differentiation and stimulates lipolysis in 3T3-L1 adipocytes[J].Metabolism,2016,65(5):646-654.  

[17] PARK S,BAEK K,CHOI C.Suppression of adipogenic differentiation by muscle cell-induced decrease in genes related to lipogenesis in muscle and fat co-culture system[J].Cell Biology International,2013,37(9):1003-1009.  

[18] HAN H Y,WEI W,CHU W W,et al.Muscle conditional medium reduces intramuscular adipocyte differentiation and lipid accumulation through regulating insulin signaling[J].International Journal of Molecular Sciences,2017,18(8):1799.
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