Molecular Nutrition

Effects of Dietary Crude Protein Level on Activities of Amino Acid Metabolic Enzymes and mRNA Expression of Amino Acid Transporters in Liver of Weaned Piglets

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  • 1. Laboratory of Bio-Feed and Molecular Nutrition, Southwest University, Chongqing 400715, China;
    2. College of Animal Science and Technology, Southwest University, Chongqing 400715, China

Received date: 2017-10-18

  Online published: 2018-04-03

Abstract

The aim of this study was to determine the effects of dietary crude protein (CP) level on activities of amino acid metabolic enzymes and mRNA expression of amino acid transporters in liver of weaned piglets. Fifty-four 28-days-old healthy "Duroc×Landrace×Yorkshire" hybrid weaned piglets (males and females in half) with the similar body weight[(7.0±0.5) kg] were randomly allotted to 3 groups[20.0% CP group (control group), 17.0% CP group and 14.0% CP group], and 18 replicates per group and 1 pig per replicate. The pre-trial lasted for 7 days, and the trial lasted for 45 days. Six piglets were selected from each group and slaughtered at the 10th day, the 25th day and the 45th day, respectively. The results showed as follows:1) at the 10th day, the activity of glutamic-oxaloacetic transaminase (GOT) in liver of weaned piglets in groups 14% and 17% CP was significantly lower than that in control group (P<0.05), and the activity of glutamine synthetase (GS) in liver in group 14% CP was significantly lower than that in the other 2 group (P<0.05). At the 25th day, the activities of GOT and GS in liver in group 14% CP were significantly lower than those in control group (P<0.05), and the activities of glutamic-pyruvic transaminase (GPT) and glutamic acid dehydrogenase (GDH) in liver in groups 14% and 17% CP were significantly lower than those in control group (P<0.05). At the 45th day, the activities of GPT and GS in liver in group 14% CP were significantly lower than those in the other 2 groups (P<0.05). 2) At 25th day, the mRNA relative expressions of solute carrier family 6 member 15 (SLC6A15) and solute carrier family 38 member 2 (SLC38A2) in liver of weaned piglets in group 14% CP were significantly lower than those in control group (P<0.05), and the mRNA relative expression of solute carrier family 36 member 1 (SLC36A1) in liver in groups 14% and 17% CP was significantly lower than that in control group (P<0.05). At 45th day, the mRNA relative expressions of solute carrier family 6 member 20 (SLC6A20) and SLC38A2 in liver in groups 14% and 17% CP were significantly lower than those in control group (P<0.05), and the mRNA relative expression of SLC6A15 in liver in group 14% CP was significantly lower than that in the other 2 group (P<0.05). In conclusion, reducing dietary CP level by 3% and 6% can reduce the activities of amino acid metabolic enzymes and the mRNA relative expressions of amino acid transporters in liver of weaned piglets.

Cite this article

ZHANG Xiangxin, CHEN Cheng, TANG Zhiru, ZHEN Jifu, XU Qingqing, SUN Zhihong . Effects of Dietary Crude Protein Level on Activities of Amino Acid Metabolic Enzymes and mRNA Expression of Amino Acid Transporters in Liver of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2018 , 30(4) : 1431 -1439 . DOI: 10.3969/j.issn.1006-267x.2018.04.026

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