Molecular Nutrition

Effects of Compound Probiotics on Nutrient Apparent Availability, Serum Biochemical Indexes and Intestinal Mucosa Morphological Structure of Broilers

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  • College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China

Received date: 2017-09-30

  Online published: 2018-04-03

Abstract

This experiment was conducted to determine the effects of different proportions of compound probiotics on nutrient apparent availability, serum biochemical indexes and intestinal mucosa morphological structure of broilers. A total of 320 healthy 1-day-old male Arbor Acres (AA) broilers were randomly divided into 4 groups. Each group has 4 replicates and each replicate included 20 broilers. Group Ⅰ was control group, and broilers in this group were fed a basal diet. Groups Ⅱ, Ⅲ and Ⅳ were experimental groups, and broilers in these groups were fed the basal diet+1 000 mg/kg compound probiotics, and the Bacillus subtilis:Saccharomyces cerevisiae:Lactobacillus acidophilus:Bifidobacterium lactis in the compound probiotics was 2:1:1:1, 1:2:1:1 and 1:1:1.5:1.5, respectively. The experiment lasted for 42 days. The results showed as follows:1) the dry matter, crude protein, ether extract and calcium apparent availability of groups Ⅱ, Ⅲ and Ⅳ was significantly higher than that of control group (P<0.05). 2) At the age of 42 days, the content of serum total protein of groups Ⅱ, Ⅲ and Ⅳ was significantly higher than that of control group (P<0.05). 3) The villus height, crypt depth and villus height/crypt depth in duodenum, jejunum and ileum of groups Ⅱ, Ⅲ and Ⅳ were better than those of control group (P>0.05). Therefore, adding different proportion of compound probiotics in the basal diet can improve the nutrient apparent availability, serum biochemical indexes and intestinal mucosa morphological structure.

Cite this article

XIE Wenhui, JIANG Ning, WANG Xin, ZHANG Aizhong . Effects of Compound Probiotics on Nutrient Apparent Availability, Serum Biochemical Indexes and Intestinal Mucosa Morphological Structure of Broilers[J]. Chinese Journal of Animal Nutrition, 2018 , 30(4) : 1495 -1503 . DOI: 10.3969/j.issn.1006-267x.2018.04.034

References

[1] 赵朋超,王建华,权春善,等.枯草芽孢杆菌抗菌肽生物合成的研究进展[J].中国生物工程杂志,2010(10):108-113.

[2] 孙锡凤.海洋红酵母对肉鸡生产性能、屠体性能及养分表现利用率的影响[D].硕士学位论文.邯郸:河北工程大学,2016.

[3] 赵建文,周明东,周霞等.微生态制剂对肉鸡代谢和免疫系统的影响[J].中国兽医学报,2012(2):216-219.

[4] 栾超.利用SUMO融合技术在枯草芽孢杆菌中重组表达抗菌肽cathelicidin-BF及其生物学活性研究[D].博士学位论文.杭州:浙江大学,2014.

[5] 黄福佳.WB800N-CC31工程菌发酵产物对大鼠生长、消化、肠道菌群及免疫的影响[D].硕士学位论文.大庆:黑龙江八一农垦大学,2016.

[6] 任善茂,陈桂银.饲料分析与检测[M].2版.北京:中国农业大学出版社,2008.

[7] 高权利,赵西莲,徐正平,等.不同日粮组成对肉仔鸡养分表观代谢率的影响[J].动物科学与动物医学,2003,20(2):55-56.

[8] HOOPER L V,MACPHERSON A J.Immune adaptations that maintain homeostasis with the intestinal microbiota[J].Nature Reviews Immunology,2010,10(3):159.

[9] 孙锡风,蒋万春,白莹,等.复合益生菌对肉鸡生产性能、屠体性能及养分表观利用率的影响[J].中国饲料,2017(4):17-21.

[10] MOUNTZOURIS K C,TSITRSIKOS P,PALAMIDI I,et al.Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility,plasma immunoglobulins,and cecal microflora composition[J].Poultry Science,2010,89(1):58.

[11] ANABE K,KAGEYAMA Y,MORIMOTO T,et al.Improved production of secreted heterologous enzyme in Bacillus subtilis strain MGB874 via modification of glutamate metabolism and growth conditions[J].Microbial Cell Factories,2013,12(1):1-10.  

[12] 李卫芬,白洁,李雅丽,等.枯草芽孢杆菌对肉鸡肉品质、养分消化率及血清生化指标的影响[J].中国兽医学报,2014,34(10):1682-1685.

[13] 侯瑛倩,杨文娇,吴振,等.酵母细胞壁多糖在养殖业上的研究应用[J].北方牧业,2013(18):31.

[14] 侯振平,吴端钦,蒋桂韬,等.不同蛋白酶对肉鸡生长性能、血清生化指标和抗氧化功能的影响[J].饲料研究,2017(14):13-17.

[15] 李金魁,陈东红.黄牛血清蛋白质含量的实验室测定[J].黄牛杂志,2002(3):22-23.

[16] DHANALAKSHMI S,DEVI R S, SRIKUMAR R,et al.Protective effect of Triphala on cold stress-induced behavioral and biochemical abnormalities in rats[J].药学杂志,2007,127(11):1863-1867.

[17] 姚淑兰,张爱忠,姜宁,等.益生素对育成期贵妃鸡养分利用率、血清生化指标及肠道微生物区系的影响[J].黑龙江畜牧兽医,2009(9):113-115.

[18] 亓秀晔,穆熙军,刘秀侠,等.复合益生菌对冷应激肉鸡血清生化指标的影响[J].饲料与畜牧(新饲料),2017(5):48-50.

[19] 唐志刚,王俊峰,温超,等.益生菌对肉鸡生产性能、免疫器官指数和血清指标的影响[J].江苏农业科学,2010(4):208-210.

[20] 刘小龙,乔家运,范寰,等.抗菌肽、合生素对AA肉鸡血清生化指标和肠道菌群的影响[J].黑龙江畜牧兽医,2017(5):111-114.

[21] 孔路军,郑雅文,赖长华,等.EM和酸化剂配合添加对肉仔鸡消化酶活性和血液生化指标及钙磷代谢的影响[J].山东家禽,2003(6):10-13.

[22] 陈静,谢全喜,刘乃芝,等.复合微生态制剂与饲用抗生素对肉鸡血清生化指标和肠道酶活性的影响[J].畜牧与饲料科学,2012,33(3):15-18.

[23] 张晓宏,孙长颢.断乳后饲料构成对大鼠肝脏CPT-Ⅰ mRNA表达影响[J].中国公共卫生,2006,22(5):563-565.

[24] 杨海英,杨在宾,杨维仁,等.益生素和低聚木糖对断奶仔猪生产性能和肠道形态学影响研究[J]中国粮油学报,2008,23(1):116-120.

[25] 祁凤华,杨帆,马红,等.枯草芽孢杆菌与嗜酸乳杆菌对黄羽肉鸡小肠黏膜形态和免疫器官指数的影响[J].黑龙江畜牧兽医,2015(19):129-131.

[26] 张彩凤,王晓翠,张海军,等.乳酸菌和酵母菌复合制剂对肉仔鸡生长性能、屠宰性能和肠道健康的影响[J].动物营养学报,2017,29(4):1248-1256.

[27] LETELLIER A,MESSIER S,LESSARD L,et al.Assessment of various treatments to reduce carriage of Salmonella in swine[J].Canadian Journal of Veterinary Research,2000,64(1):27.

[28] LAM E K,YU L,WONG H P,et al.Probiotic Lactobacillus rhamnosus GG enhances gastric ulcer healing in rats[J].European Journal of Pharmacology,2007,565(1/2/3):171-179.
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