Effects of Exogenous Amylase on in Vitro Nutrient Digestibility and Metabolic Energy of Corn-Soybean Meal Type Diets for Broilers

ZHANG Xian, ZHANG Leying, ZHANG Lilan, CHEN Liang, TANG Xiangfang, ZHANG Hongfu

doi:10.3969/j.issn.1006-267x.2018.04.036

2018 , Vol. 30 >Issue 4: 1513 - 1521

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.04.036

Molecular Nutrition

Effects of Exogenous Amylase on in Vitro Nutrient Digestibility and Metabolic Energy of Corn-Soybean Meal Type Diets for Broilers

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. School of Life and food Engineering, Hebei University of Engineering, Handan 056000, China

Received date: 2017-09-22

Online published: 2018-04-03

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Abstract

This experiment was conducted to investigate the effects of exogenous amylase on in vitro nutrient digestibility and metabolic energy of corn-soybean meal type diets for broilers using a computer-controlled simulated digestion system (SDS-Ⅱ), in order to provide a reference of the establishing in vitro method for efficacy evaluation of exogenous enzyme. A 2×4 factorial arrangement design was adopted in the present study. Two corn-soybean meal type diets were formulated to meet the nutrient requirements of broilers at 1 to 21 and 22 to 42 days of age according to Feeding Standard of Chicken of China (NY/T 33-2004) and NRC (1994), respectively. Six amylase supplementation diets were formulated by adding 1 840, 9 200 and 18 400 U/g exogenous amylase into the two basal diets, respectively. The two basal diets without exogenous amylase were used as control. The in vitro dry matter digestibility (IVDMD), in vitro apparent crude protein digestibility (IVACPD), in vitro standardized crude protein digestibility (IVSCPD), in vitro starch digestibility (IVSTD), in vitro gross energy digestibility (IVGED) and in vitro metabolic energy (IVME) of the 8 diets were determined by SDS-Ⅱ. Each diet contained 5 replicates with 1 digestion tube per replicate. The results showed as follows:1) compared with the control group, the IVDMD and IVGED in gastric stage of diets in 1 840, 9 200 and 18 400 U/g amylase groups were significantly increased (P<0.05). The IVME of diets in 18 400 U/g amylase group was significantly higher than that in the other groups (P<0.05). The IVDMD and IVGED in total digestive tract and IVME of the 22 to 42 days of age diet were significantly higher than those in the 22 to 42 days of age diet (P<0.05). 2) The IVACPD and IVSCPD in total digestive tract of diets in 1 840 and 9 200 U/g amylase groups were significantly higher than those in the control group (P<0.05). The IVACPD and IVSCPD in total digestive tract of diets in the 22 to 42 days of age diet were significantly higher than those in the 1 to 21 days of age diet (P<0.05). 3) The IVSTD in total digestive tract of 8 diets were all more than 99.40%, and it in 9 200 and 18 400 U/g amylase groups was significantly lower than that in the control group (P<0.05). The IVSTD in total digestive tract in the 22 to 42 days of age diet was significantly higher than that in the 1 to 21 days of age diet (P<0.05). 4) There were interaction effects between dietary nutrient level and amylase additional dose on the IVDMD, IVGED, IVACPD, IVSCPD, IVSTD and IVME were observed in the (P<0.01). It is concluded that the addition of exogenous amylase increases the IVDMD and IVDGE in gastric stage, the addition of 9 200 U/g amylase increases the IVACPD and IVSCPD, and the addition of 18 400 U/g amylase increases the IVME of diets for broilers. The starch in the corn-soybean meal type diets was almost completely degraded and no effect of amylase on the IVSTD. There are interaction effects of dietary nutrient level and amylase additional dose on the in vitro nutrient digestibility and metabolic energy. The in vitro nutrient digestibility and metabolic energy in the 1 to 21 days of age diet are higher than those in the 22 to 42 days of age diet.

Key words： exogenous amylase; SDS-Ⅱ; nutrient; digestibility; metabolic energy

Cite this article

ZHANG Xian, ZHANG Leying, ZHANG Lilan, CHEN Liang, TANG Xiangfang, ZHANG Hongfu . Effects of Exogenous Amylase on in Vitro Nutrient Digestibility and Metabolic Energy of Corn-Soybean Meal Type Diets for Broilers[J]. Chinese Journal of Animal Nutrition, 2018 , 30(4) : 1513 -1521 . DOI: 10.3969/j.issn.1006-267x.2018.04.036

References

[1] 张宏福,赵峰,张子仪.仿生消化法评定猪饲料生物学效价的研究进展[C]//2010中国畜牧兽医学会动物营养学分会第六次全国饲料营养学术研讨会论文集.杨凌:中国畜牧兽医学会,2010:5-9.

[2] 陈亮,张宏福,赵峰.体外透析法评定单胃动物饲料养分生物学效价[J].饲料工业,2013(19):58-61.

[3] GRACIA M I,ARANIBAR M J,LAZARO R,et al.Alpha-amylase supplementation of broiler diets based on corn[J].Poultry Science,2003,82(3):436-442.

[4] ONDERCI M,SAHIN N,SAHIN K,et al.Efficacy of supplementation of alpha-amylase-producing bacterial culture on the performance,nutrient use,and gut morphology of broiler chickens fed a corn-based diet[J].Poultry Science,2006,85(3):505-510.

[5] JIANG Z Y,ZHOU Y M,LU F Z,et al.Effects of different levels of supplementary alpha-amylase on digestive enzyme activities and pancreatic amylase mRNA expression of young broilers[J].Asian Australasian Journal of Animal Sciences,2008,21(1):97-102.

[6] KACZMAREK S A,ROGIEWICZ A,MOGIELNICKA M,et al.The effect of protease,amylase,and nonstarch polysaccharide-degrading enzyme supplementation on nutrient utilization and growth performance of broiler chickens fed corn-soybean meal-based diets[J].Poultry Science,2014,93(7):1745-1753.

[7] MAHAGNA M,NIR I,LARBIER M,et al.Effect of age and exogenous amylase and protease on development of the digestive tract,pancreatic enzyme activities and digestibility of nutrients in young meat-type chicks[J].Reproduction Nutrition Development,1995,35(2):201-212.

[8] YEGANI M,KORVER D R.Effects of corn source and exogenous enzymes on growth performance and nutrient digestibility in broiler chickens[J].Poultry Science,2013,92(5):1208-1220.

[9] ALABI O O,ATTEH J O,ADEJUMO I O.Comparative evaluation of in vitro and in vivo nutrient digestibility of dietary levels of rice husk supplemented with or without commercial enzyme[J].International Journal of Research in Agriculture and Forestry,2015,2(6):15-19.

[10] MALATHI V,DEVEGOWDA G.In vitro evaluation of nonstarch polysaccharide digestibility of feed ingredients by enzymes[J].Poultry Science,2001,80(3):302-305.

[11] PARK K R,CHAN S P,KIM B G.An enzyme complex increases in vitro dry matter digestibility of corn and wheat in pigs[J].SpringerPlus,2016,5:598.

[12] 张立兰,陈亮,钟儒清,等.外源蛋白酶对肉鸡饲粮体外干物质消化率和酶水解物能值的影响[J].中国农业科学,2017,50(7):1326-1333.

[13] 张立兰,高理想,陈亮,等.体外消化法优化生长猪玉米-豆粕-DDGS饲粮和小麦-豆粕饲粮非淀粉多糖酶谱的研究[J].畜牧兽医学报,2017,48(8):1468-1480.

[14] 中华人民共和国农业部.NY/T 33-2004鸡饲养标准[S].北京:中国农业出版社,2004.

[15] NRC.Nutrient requirements of poultry[S].9th ed.Washington,D.C.:National Academy Press,1994:27.

[16] 赵峰,张宏福,张子仪.单胃动物仿生消化系统操作手册[M].2版.北京:中国农业科学院,2011.

[17] 刘永超,刘宁,石学刚.低能日粮添加木聚糖酶或复合酶对肉鸡生产性能和养分消化率的影响[J].饲料工业,2011,32(16):39-42.

[18] 黄学琴.复合酶制剂对肉鸭生产性能、养分利用率及钙磷代谢的影响[D].硕士学位论文.雅安:四川农业大学,2013.

[19] 蒋正宇,周岩民,王恬.外源α-淀粉酶对21日龄肉鸡消化器官发育、肠道内源酶活性的影响[J].畜牧兽医学报,2007,38(7):672-677.

[20] 张仕琦,热合木塔依·吾布力卡斯木,陈跃平,等.低温α-淀粉酶对青脚麻鸡生长性能和消化功能的影响[J].粮食与饲料工业,2016,12(8):57-60.

[21] NOY Y,SKLAN D.Digestion and absorption in the young chick[J].Poultry Science,1995,74(2):366-373.

[22] AO T,CANTOR A H,PESCATORE A J,et al.In vitro evaluation of feed-grade enzyme activity at pH levels simulating various parts of the avian digestive tract[J].Animal Feed Science and Technology,2008,140(3/4):462-468.

[23] 刘慧龙.复合酶对几种饲料原料养分消化率的影响及其在猪饲料中的应用[D].硕士学位论文.沈阳:沈阳农业大学,2016.

[24] 蒋正宇.外源α-淀粉酶对肉鸡消化器官发育、内源酶活性的影响及后续效应的研究[D].硕士学位论文.南京:南京农业大学,2006.

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