Molecular Nutrition

Inhibitory Effect of Clostridium butyricum on Intestinal Damage of Lipopolysaccharide-Induced Acute Stress Rats

Expand
  • 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received date: 2017-10-16

  Online published: 2018-04-03

Abstract

This experiment was conducted to investigate the effects of Clostridium butyricum on the growth performance of healthy rats and the intestinal structure, intestinal disaccharidase activities and intestinal inflammation of lipopolysaccharide (LPS)-induced acute stress rats. Thirty six male SD rats were randomly allocated to control group, LPS group, LPS + Clostridium butyricum group. Control group and LPS group were fed a basal diet, LPS + Clostridium butyricum group were fed the basal diet with the addition of 0.05% Clostridium butyricum. On day 40 of experiment, LPS group and LPS + Clostridium butyricum group were given an intraperitoneal injection of 4 mg/kg BW LPS (LPS concentration was 1.5 mg/mL and the injection volume about 1 mL). Rats in control group were given an intraperitoneal injection of 1 mL saline solution. Three hours later, all rats were sacrificed to collect samples. The results were as follows:1) Clostridium butyricum showed no significant effects on final weight, average daily gain, average daily feed intake and feed/gain of healthy rats (P>0.05). 2) Injection of LPS significantly increased crypt depth in duodenum and significantly decreased villus height/crypt depth in duodenum, mucosal thickness in jejunum, intraepithelial lymphocyte (IEL) count in jejunum and ileum as compared with control group (P<0.05). Preventive feeding Clostridium butyricum significantly decreased crypt depth in duodenum and significantly increased mucosal thickness and IEL count in jejunum as compared with LPS group (P<0.05). 3) Injection of LPS significantly decreased sucrase activity in duodenum and maltase activity in jejunum as compared with control group (P<0.05). Preventive feeding Clostridium butyricum suppressed the decrease of sucrase activity in duodenum and the sucrase activity in duodenum in LPS + Clostridium butyricum group showed no significant difference as compared with control group (P>0.05). 4) Injection of LPS significantly increased of myeloperoxidase (MPO) activity, the contents interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in jejunum and ileum as compared with control group (P<0.05). Preventive feeding Clostridium butyricum significantly decreased MPO activity in jejunum and ileum, and the contents of IL-6 and TNF-α in jejunum as compared with LPS group (P<0.05). Meantime, Clostridium butyricum suppressed the increase of IL-6 and TNF-α contents in ileum and showed no significant difference to control group (P>0.05). In conclusion, Clostridium butyricum shows no negative effects on growth performance of healthy rats. For LPS-induced acute stress rats, pretreatment of Clostridium butyricum can suppress the decrease of intestinal sucrase activity, ameliorate the damage of intestinal mucosal structure, improve intestinal immune function and decrease intestinal inflammation.

Cite this article

WU Jie, DENG Bo, LI Xiaohui, XU Ziwei . Inhibitory Effect of Clostridium butyricum on Intestinal Damage of Lipopolysaccharide-Induced Acute Stress Rats[J]. Chinese Journal of Animal Nutrition, 2018 , 30(4) : 1530 -1537 . DOI: 10.3969/j.issn.1006-267x.2018.04.038

References

[1] ROSS G R,GUSILS C,OLISZEWSKI R,et al.Effects of probiotic administration in swine[J].Journal of Bioscience & Bioengineering,2010,109(6):545-549.  

[2] GIANG H H,VIET T Q,OGLE B,et al.Growth performance,digestibility,gut environment and health status in weaned piglets fed a diet supplemented with a complex of lactic acid bacteria alone or in combination with Bacillus subtilis and Saccharomyces boulardii[J].Livestock Science,2012,143(2/3):132-141.

[3] ARAKI Y,ANDOH A,FUJIYAMA Y,et al.Oral administration of a product derived from Clostridium butyricum in rats[J].International Journal of Molecular Medicine,2002,9(1):53-57.

[4] OKAMOTO T,SASAKI M,TSUJIKAWA T,et al.Preventive efficacy of butyrate enemas and oral administration of Clostridium butyricum M588 in dextran sodium sulfate-induced colitis in rats[J].Journal of Gastroenterology,2000,35(5):341-346.  

[5] BUCHHOLZ B M,KACZOROWSKI D J,SUGIMOTO R,et al.Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury[J].American Journal of Transplantation,2008,8(10):2015-2024.  

[6] KANG J H,YUN S I,PARK M H,et al.Anti-obesity effect of Lactobacillus gasseri BNR17 in high-sucrose diet-induced obese mice[J].PLoS One,2012,8(1):e54617.

[7] MENG Q W,YAN L,AO X,et al.Influence of probiotics in different energy and nutrient density diets on growth performance,nutrient digestibility,meat quality,and blood characteristics in growing-finishing pigs[J].Journal of Animal Science,2010,88(10):3320-3326.  

[8] CAO G T,XIAO Y P,YANG C M,et al.Effects of Clostridium butyricum on growth performance,nitrogen metabolism,intestinal morphology and cecal microflora in broiler chickens[J].Journal of Animal and Veterinary Advances,2012,11(15):2665-2671.  

[9] KUO S M,MERHIGE P M,HAGEY L R.The effect of dietary prebiotics and probiotics on body weight,large intestine indices,and fecal bile acid profile in wild type and IL10-/- mice[J].PLoS One,2013,8(3):e60270.

[10] 王腾浩.新型丁酸梭菌筛选及其对断奶仔猪生长性能和肠道功能影响的研究[D].博士学位论文.杭州:浙江大学,2015.

[11] 刘婷婷.丁酸梭菌对肉鸡生产性能、小肠形态、免疫功能和肠道微生物的影响[D].硕士学位论文.杭州:浙江大学,2011.

[12] ICHIKAWA H,KUROIWA T,INAGAKI A,et al.Probiotic bacteria stimulate gut epithelial cell proliferation in rat[J].Digestive Diseases and Sciences,1999,44(10):2119-2123.  

[13] ASAI K I,KOMINE Y,KOZUTSUMI T,et al.Predominant subpopulations of T lymphocytes in the mammary gland secretions during lactation and intraepithelial T lymphocytes in the intestine of dairy cows[J].Veterinary Immunology and Immunopathology,2000,73(3/4):233-240.

[14] 杨倩,张小飞.淋巴细胞在黏膜上皮中的运动[J].解剖学杂志,2005,28(2):145-148.

[15] RIEGER J,JANCZYK P,HVNIGEN H,et al.Intraepithelial lymphocyte numbers and histomorphological parameters in the porcine gut after Enterococcus faecium NCIMB 10415 feeding in a Salmonella typhimurium challenge[J].Veterinary Immunology & Immunopathology,2015,164(1/2):40-50.

[16] BAI S P,WU A M,DING X M,et al.Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens[J].Poultry Science,2013,92(3):663-670.  

[17] LEE S H,YU S Y,NAKAYAMA J,et al.Core2O-glycan structure is essential for the cell surface expression of sucrase isomaltase and dipeptidyl peptidase-Ⅳ during intestinal cell differentiation[J].The Journal of Biological Chemisty,2010,285(48):37683-37692.  

[18] 窦茂鑫.不同类型益生菌制剂对脂多糖刺激仔猪肠道吸收和屏障功能的影响[D].硕士学位论文.武汉:武汉轻工大学,2013.

[19] GOYAL N,RISHI P,SHUKLA G.Lactobacillus rhamnosus GG antagonizes Giardia intestinalis induced oxidative stress and intestinal disaccharidases:an experimental study[J].World Journal of Microbiology and Biotechnology,2013,29(6):1049-1057.  

[20] CHEN J W,CHEN Y H,LIN S J.Long-term exposure to oxidized low-density lipoprotein enhances tumor necrosis factor-α-stimulated endothelial adhesiveness of monocytes by activating superoxide generation and redox-sensitive pathways[J].Free Radical Biology and Medicine,2006,40(5):817-826.  

[21] RAVAGLIA G,FORTI P,MAIOLI F,et al.Associations of the-174 G/C interleukin-6 gene promoter polymorphism with serum interleukin 6 and mortality in the elderly[J].Biogerontology,2005,6(6):415-423.  

[22] ZHANG H Q,DING T T,ZHAO J S,et al.Therapeutic effects of Clostridium butyricum on experimental colitis induced by oxazolone in rats[J].World Journal of Gastroenterology,2009,15(15):1821-1828.  

[23] 庞敏,卢庆萍,朱丽媛,等.益生菌抵御大鼠急性腹泻的效果及其对肠道黏膜屏障的影响[J].动物营养学报,2016,28(5):1462-1470.

[24] CARDINAL J S,ZHAN J H,WANG Y N,et al.Oral hydrogen water prevents chronic allograft nephropathy in rats[J].Kidney International,2010,77(2):101-109.  

[25] HAYASHI A,SATO T,KAMADA N,et al.A single strain of Clostridium butyricum induces intestinal IL-10-producing macrophages to suppress acute experimental colitis in mice[J].Cell Host & Microbe,2013,13(6):711-722.  
Outlines

/