Effects of Different Nitrogen/Energy Diets on Biochemical Parameters in Amniotic Fluid and Allantoic Fluid of Pregnant Huanjiang Mini-Pigs

ZHAO Yue, KONG Xiangfeng, JI Yujiao, GENG Meimei, LI Huawei, WANG Zhanbin

doi:10.3969/j.issn.1006-267x.2018.04.043

2018 , Vol. 30 >Issue 4: 1574 - 1581

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.04.043

Molecular Nutrition

Effects of Different Nitrogen/Energy Diets on Biochemical Parameters in Amniotic Fluid and Allantoic Fluid of Pregnant Huanjiang Mini-Pigs

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1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;
2. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
3. Research Center of Mini-Pig, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, China

Received date: 2017-09-30

Online published: 2018-04-03

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Abstract

The present study was conducted to investigate the effects of different nitrogen/energy diets on biochemical parameters in amniotic fluid and allantoic fluid of pregnant Huanjiang mini-pigs. Forty-eight primiparous Huanjiang mini-pigs were randomly assigned into one of two groups and reared in 8 pens (replicates) with 3 pigs per pen. After service, the animals in the two groups were fed high nitrogen/energy diet (digestible energy was 14.73 MJ/kg, crude protein content was 13.11%, and nitrogen/energy was 0.89) and low nitrogen/energy diet (digestible energy was 12.24 MJ/kg, crude protein content was 9.77%, and nitrogen/energy was 0.80), respectively. At 45, 75, and 110 days of pregnancy, one pig was selected randomly from each pen and sacrificed. The amniotic fluid and allantoic fluid of fetuses with maximal body weight (BW), middle BW and minimal BW per litter were collected for determining biochemical parameters. The results showed that under the same pregnant time and standard body weight conditions, compared with feeding the low nitrogen/energy diet, feeding the high nitrogen/energy diet significantly increased the alkaline phosphatase (ALP) activity in allantoic fluid of fetuses with minimal BW at 75 days of pregnancy(P<0.05), while significantly decreased the aspartate aminotransferase (AST) activity in allantoic fluid of fetuses with middle or minimal BW at 75 days of pregnancy and fetuses with minimal BW at 110 days of pregnancy, the uric acid (UA) content of fetuses with maximal BW at 45 and 75 days of pregnancy, the creatine kinase (CK) activity of fetuses with middle or minimal BW at 45 days of pregnancy and fetuses with maximal BW at 75 days of pregnancy (P<0.05). Compared with the fetuses with minimal BW, the albumin (ALB) content in amniotic fluid of fetuses with maximal BW at 45 and 75 days of pregnancy in high nitrogen/energy diet group was significantly increased (P<0.05), as well as at 45 and 110 days of pregnancy in low nitrogen/energy diet group (P<0.05). With the pregnant time extension, the urea (UREA) and UA contents in amniotic fluid and UA content in allantoic fluid of fetuses in two diet groups were gradually increased. These findings suggested that the high nitrogen/energy diet can promote the growth and development of fetuses by regulating the biochemical parameters in amniotic fluid and allantoic fluid; the fetuses with maximal BW presents higher utilization efficiency of protein than the fetuses with middle or minimal BW.

Key words： Huanjiang mini-pigs; pregnant time; dietary nitrogen/energy; amniotic fluid; allantoic fluid; biochemical parameters

Cite this article

ZHAO Yue, KONG Xiangfeng, JI Yujiao, GENG Meimei, LI Huawei, WANG Zhanbin . Effects of Different Nitrogen/Energy Diets on Biochemical Parameters in Amniotic Fluid and Allantoic Fluid of Pregnant Huanjiang Mini-Pigs[J]. Chinese Journal of Animal Nutrition, 2018 , 30(4) : 1574 -1581 . DOI: 10.3969/j.issn.1006-267x.2018.04.043

References

[1] 张博生,马君峰.中国小型猪种——香猪[J].畜牧兽医杂志,2015,34(4):79,81.

[2] 郭海燕,吴德,张明,等.日粮不同营养水平对母猪妊娠早期胚胎存活的影响[J].中国畜牧杂志,2007,43(17):30-33.

[3] 刘俊锋,吴琛,孔祥峰,等.精氨酸对妊娠环江香猪胎儿生长发育的影响[J].中国农业科学,2011,44(5):1040-1045.

[4] REGMI N,WANG T J,CRENSHAW M A,et al.Effects of dietary lysine levels on plasma free amino acid profile in late-stage finishing pigs[J].SpringerPlus,2016,5(1):888.

[5] YANG H S,FU D Z,SHAO H,et al.Impacts of birth weight on plasma,liver and skeletal muscle neutral amino acid profiles and intestinal amino acid transporters in suckling Huanjiang mini-piglets[J].PLoS One,2012,7(12):e50921.

[6] WANG J J,CHEN L X,LI D F,et al.Intrauterine growth restriction affects the proteomes of the small intestine,liver,and skeletal muscle in newborn pigs[J].The Journal of Nutrition,2008,138(1):60-66.

[7] UNDERWOOD M A,GILBERT W M,SHERMAN M P.Amniotic fluid:not just fetal urine anymore[J].Journal of Perinatology,2005,25(5):341-348.

[8] LI N,WELLS D N,PETERSON A J,et al.Perturbations in the biochemical composition of fetal fluids are apparent in surviving bovine somatic cell nuclear transfer pregnancies in the first half of gestation[J].Biology of Reproduction,2005,73(1):139-148.

[9] WANG J,JIANG F,ZHANG J,et al.Amniotic fluid metabolomics and biochemistry analysis provides novel insights into the diet-regulated foetal growth in a pig model[J].Scientific Reports,2017,7:44782.

[10] MATEO R D,WU G Y,BAZER F W,et al.Dietary L-arginine supplementation enhances the reproductive performance of gilts[J].Journal of Nutrition,2007,137(3):652-656.

[11] 祝倩,姬玉娇,李华伟,等.高、低营养水平饲粮对妊娠环江香猪繁殖性能、体成分和血浆生化参数的影响[J].动物营养学报,2016,28(5):1534-1540.

[12] 祝倩,马思聪,姬玉娇,等.不同氮能比饲粮对妊娠环江香猪脐带血管发育相关基因表达的影响[J].动物营养学报,2017,29(2):613-619.

[13] 王秋颖.碱性磷酸酶特性及其应用的研究进展[J].中国畜牧兽医,2011,38(1):157-161.

[14] 王典,李发弟,张养东,等.马铃薯淀粉渣-玉米秸秆混合青贮料对肉羊生产性能、瘤胃内环境和血液生化指标的影响[J].草业学报,2012,21(5):47-54.

[15] 陈光亮,徐叔云.高尿酸血症研究进展[J].中国药理学通报,2003,19(10):1088-1092.

[16] 汪翼.肌酸激酶升高的临床意义与特发性高肌酸激酶血症[J].中国实用儿科杂志,2009,24(6):494-496.

[17] 王蜀金,陈惠娜,方思敏,等.功能性氨基酸在动物机体内的代谢利用与生理功能[J].家畜生态学报,2014,35(8):6-12.

[18] 刘俊锋,吴琛,孔祥峰,等.不同体重环江香猪胎儿的羊水和尿囊液化学成分分析[J].中国农业科学,2011,44(19):4066-4071.

[19] 张勇,张雄,陆静,等.两种中草药添加剂对从江香猪仔猪生长性能及血清免疫指标的影响[J].中国畜牧杂志,2017,53(2):127-131.

[20] 孔祥峰,伍国耀,印遇龙.猪宫内生长迟缓及其防治研究进展[J].畜牧与兽医,2009,41(10):96-101.

[21] PAWLIOWSKY K,ERNST L,STEITZ J,et al.The aachen minipig:phenotype,genotype,hematological and biochemical characterization,and comparison to the göttingen minipig[J].Karger,2017,58(5/6):193-203.

[22] 李博,李伟,张昊,等.日粮胆碱水平与宫内发育迟缓对猪肝脏抗氧化能力的影响[J].食品工业科技,2015,36(7):363-366,376.

[23] WU G Y,BAZER F W,HU J B,et al.Polyamine synthesis from proline in the developing porcine placenta[J].Biology of Reproduction,2005,72(4):842-850.

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