Molecular Nutrition

Effects of Recombinant Bacillus subtilis SE1 on Growth Performance, Intestinal Digestive Enzyme Activities and Microflora of Broilers

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  • 1. Animal Microecology Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
    2. Chengdu Agricultural College, Chengdu 611130, China;
    3. Chengdu Zhongmu Biological Pharmaceutical Co., Ltd., Chengdu 610000, China

Received date: 2017-10-16

  Online published: 2018-04-03

Abstract

This experiment was conducted to investigate the effects of recombinant Bacillus subtilis SE1 spore surface display of Salmonella pullorum OmpC on growth performance, intestinal digestive enzyme activities and microflora of broilers. One hundred and twenty seven-day-old broilers were randomly divided into 3 groups with 4 replicates per group and 10 broilers per replicate. Broilers in group A (control group) were fed a basal diet, and the others in groups B and C were fed the basal diets supplemented with 0.1% (1.0×106 CFU/g) Bacillus subtilis 168 and 0.1% (1.0×106 CFU/g) recombinant Bacillus subtilis SE1, respectively. The experiment lasted for 35 days. The results showed as follows:1) compared with group A, the final weight and average gain of broilers in group C increased by 6.14% and 6.76% (P>0.05), respectively, and ratio of feed to gain in group C decreased by 8.21% (P>0.05). 2) The activities of jejunal lipase and ileal protease of broilers in groups B and C were significantly higher than those in group A at 28 and 42 days of age (P<0.05), and there were no significant differences on intestinal digestive enzyme activities of broilers between group B and group C (P>0.05). 3) At 28 and 42 days of age, the number of Escherichia coli in ileum and cecum of broilers in groups B and C was significantly lower than that in group A (P<0.05), and the number of Lactobacillus in cecum in groups B and C was significantly higher than that in group A (P<0.05). The results of polymerase chain reaction-denatured gradient gel electrophoresis (PCR-DGGE) of 16S rRNA V3 zone showed that the richness, evenness and Shannon-Wiener index of caecal microflora of broilers in groups B and C were significantly higher than those in group A (P<0.05), the similarity of caecal microflora in group C increased by 26.8% and 15.6% compared with group A at 28 and 42 days of age, respectively, and there was no significant difference between group C and group B (P>0.05). It is concluded that recombinant Bacillus subtilis SE1 have the same effect as Bacillus subtilis 168, can effectively promote growth of broilers, improve the activities of intestinal lipase and protease, regulate the intestinal microflora of broilers, and improve the stability and diversity of intestinal microflora.

Cite this article

LI Jiajun, WANG Zhenhua, HU Zhengmao, TANG Huiqin, GU Xiaoxiao, LIAO Chengbin, PAN Kangcheng . Effects of Recombinant Bacillus subtilis SE1 on Growth Performance, Intestinal Digestive Enzyme Activities and Microflora of Broilers[J]. Chinese Journal of Animal Nutrition, 2018 , 30(4) : 1582 -1591 . DOI: 10.3969/j.issn.1006-267x.2018.04.044

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