Dietary Vitamin B6 Requirement of Genetically Improved Farmed Tilapia (Oreochromis niloticus)

WU Fan, REN Chun, WEN Hua, JIANG Ming, LIU Wei, TIAN Juan, YANG Changgeng, YU Lijuan, LU Xing

doi:10.3969/j.issn.1006-267x.2018.05.020

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 5: 1781 - 1788

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.05.020

Molecular Nutrition

Dietary Vitamin B₆ Requirement of Genetically Improved Farmed Tilapia (Oreochromis niloticus)

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1. Fish Nutrition and Feed Division, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
2. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2017-11-01

Online published: 2018-05-06

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Abstract

This experiment was conducted to estimate the effects of dietary vitamin B₆ content on growth performance, whole body and muscle nutritional composition, serum and liver biochemical indices of genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus), and to determine the dietary vitamin B₆ requirement of GIFT. A total of 360 GIFT with the average initial body weight of (56.3±1.8) g were randomly divided into 6 groups with 3 replicates per group and 20 fish per replicate. The fish were fed six purified diets which containing 0.3 (control group), 2.7, 5.4, 10.1, 20.5 and 39.8 mg/kg vitamin B₆, respectively. The experiment lasted for 10 weeks. The results showed as follow:1) with the increased of dietary vitamin B₆ content (0.3 to 10.1 mg/kg), the weight gain rate of GIFT was significantly increased (P<0.05), and then reached a plateau when dietary vitamin B₆ content reached 10.1 mg/kg. The feed conversion ratio of GIFT in 5.4, 10.1, 20.5 and 39.8 mg/kg groups were significantly lower than those in control group and 2.7 mg/kg group (P<0.05). The hepatosomatic index in 5.4 and 20.5 mg/kg groups was significantly higher than that in control group (P<0.05). 2) The contents of crude protein and ash in whole body in 2.7, 5.4, 10.1, 20.5 and 39.8 mg/kg groups were significantly higher than those in control group (P<0.05). The 10.1 mg/kg group got the lowest crude lipid content, and was significantly lower than that in control group and 2.7, 5.4 and 39.8 mg/kg groups (P<0.05). The muscle crude protein content in 5.4,10.1 and 20.5 mg/kg groups was significantly higher than that in control group (P<0.05). 3) There were no significant effects on serum glucose, total protein contents and liver aspartate aminotransferase activity among all groups (P>0.05). The serum alkaline phosphatase activity in 20.5 and 39.8 mg/kg groups was significantly higher than that in control group (P<0.05), the serum urea nitrogen content in 10.1 and 39.8 mg/kg groups was significantly higher than that in control group (P<0.05), the liver alanine aminotransferase activity in 10.1, 20.5 and 39.8 mg/kg groups was significantly higher than that in control group and 2.7, 5.4 mg/kg groups (P<0.05). In summary, the broken-line regression analysis shows that using weight rate and liver alanine aminotransferase activity as response criteria, the optimal requirements of dietary vitamin B₆ for GIFT[initial body weight (56.3±1.8) g] are 9.22 and 10.11 mg/kg, respectively.

Key words： GIFT; vitamin B₆; growth performance; biochemical indices; requirement

Cite this article

WU Fan, REN Chun, WEN Hua, JIANG Ming, LIU Wei, TIAN Juan, YANG Changgeng, YU Lijuan, LU Xing . Dietary Vitamin B₆ Requirement of Genetically Improved Farmed Tilapia (Oreochromis niloticus)[J]. Chinese Journal of Animal Nutrition, 2018 , 30(5) : 1781 -1788 . DOI: 10.3969/j.issn.1006-267x.2018.05.020

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